Research progress on application of composite DNA hydrogels in detection of mycotoxins in foods

  • ZHANG Shuming ,
  • WANG Xin ,
  • WANG Xuwu ,
  • YANG Qing ,
  • WANG Kai ,
  • WANG Qiang
Expand
  • 1(School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China)
    2(Key Laboratory of Lipid Resource Utilization and Children’s Daily Chemical Products, Chongqing University of Education, Chongqing 400067, China)

Received date: 2024-07-22

  Revised date: 2024-09-03

  Online published: 2024-12-30

Abstract

The development of detection technologies that can rapidly and efficiently detect potentially harmful substances in food is of great significance for ensuring food safety and safeguarding human health.Hydrogels with three-dimensional polymer network structures have the advantages of large specific surface area, easy structural functionalization, good biocompatibility, good flexibility, good mechanical stability, etc.Among them, composite hydrogels have attracted wide attention in the field of food safety detection.This paper reviews the synthesis and classification of composite DNA hydrogels and their application in the detection of mycotoxins in food.The different synthesis strategies are divided into physical and chemical cross-linking methods, and according to the composite type, composite DNA hydrogels can be divided into polymer composite DNA hydrogels and nanoparticle composite DNA hydrogels.This study summarizes the research progress of composite DNA hydrogel in the detection of various mycotoxins in food.Research progress and suggestions are put forward to improve the sensitivity and accuracy of food safety detection through further functionalization and intelligent design, and its development prospects are prospected.

Cite this article

ZHANG Shuming , WANG Xin , WANG Xuwu , YANG Qing , WANG Kai , WANG Qiang . Research progress on application of composite DNA hydrogels in detection of mycotoxins in foods[J]. Food and Fermentation Industries, 2024 , 50(24) : 401 -410 . DOI: 10.13995/j.cnki.11-1802/ts.040539

References

[1] KING T, COLE M, FARBER J M, et al. Food safety for food security: Relationship between global megatrends and developments in food safety[J]. Trends in Food Science & Technology, 2017, 68:160-175.
[2] WANG B, SHEN F, HE X M, et al. Simultaneous detection of Aspergillus moulds and aflatoxin B1 contamination in rice by laser induced fluorescence spectroscopy[J]. Food Control, 2023, 145:109485.
[3] 边亚兰, 李光华, 高志贤, 等. 适配体功能化的DNA水凝胶的制备及其在食品安全小分子快速检测中的应用[J]. 食品安全质量检测学报, 2023, 14(6):17-23.
BIAN Y L, LI G H, GAO Z X, et al. Preparation of aptamer functionalized DNA hydrogel and its application in the rapid detection of small molecules in food safety[J]. Journal of Food Safety & Quality, 2023, 14(6):17-23.
[4] UZUMCU A T, GUNEY O, OKAY O. Nanocomposite DNA hydrogels with temperature sensitivity[J]. Polymer, 2016, 100:169-178.
[5] LI F, TANG J P, GENG J H, et al. Polymeric DNA hydrogel: Design, synthesis and applications[J]. Progress in Polymer Science, 2019, 98:101163.
[6] EBRAHIMI A, RAVAN H, KHAJOUEI S. DNA nanotechnology and bioassay development[J]. TrAC Trends in Analytical Chemistry, 2019, 114:126-142.
[7] KHAJOUEI S, RAVAN H, EBRAHIMI A. DNA hydrogel-empowered biosensing[J]. Advances in Colloid and Interface Science, 2020, 275:102060.
[8] ZHENG M Y, LIU H M, YE J, et al. Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification[J]. Food Chemistry: X, 2022, 15:100395.
[9] TANG L Y, HUANG Y Y, LIN C Y, et al. Highly sensitive and selective aflatoxin B1 biosensor based on Exonuclease I-catalyzed target recycling amplification and targeted response aptamer-crosslinked hydrogel using electronic balances as a readout[J]. Talanta, 2020, 214:120862.
[10] LI Z Y, DAVIDSON-ROZENFELD G, VÁZQUEZ-GONZÁLEZ M, et al. Multi-triggered supramolecular DNA/bipyridinium dithienylethene hydrogels driven by light, redox, and chemical stimuli for shape-memory and self-healing applications[J]. Journal of the American Chemical Society, 2018, 140(50):17691-17701.
[11] WANG H Y, WANG X Y, LAI K Q, et al. Stimulus-responsive DNA hydrogel biosensors for food safety detection[J]. Biosensors, 2023, 13(3):320.
[12] NAGAHARA S, MATSUDA T. Hydrogel formation via hybridization of oligonucleotides derivatized in water-soluble vinyl polymers[J]. Polymer Gels and Networks, 1996, 4(2):111-127.
[13] CHENG E J, XING Y Z, CHEN P, et al. A pH-triggered, fast-responding DNA hydrogel[J]. Angewandte Chemie, 2009, 121(41):7796-7799.
[14] ZHANG L, LEI J P, LIU L, et al. Self-assembled DNA hydrogel as switchable material for aptamer-based fluorescent detection of protein[J]. Analytical Chemistry, 2013, 85(22):11077-11082.
[15] LEE J B, PENG S M, YANG D Y, et al. A mechanical metamaterial made from a DNA hydrogel[J]. Nature Nanotechnology, 2012, 7(12):816-820.
[16] XU N, MA N N, YANG X T, et al. Preparation of intelligent DNA hydrogel and its applications in biosensing[J]. European Polymer Journal, 2020, 137:109951.
[17] WU P, LI S, YE X S, et al. Cu/Au/Pt trimetallic nanoparticles coated with DNA hydrogel as target-responsive and signal-amplification material for sensitive detection of microcystin-LR[J]. Analytica Chimica Acta, 2020, 1134:96-105.
[18] XUE M X, CAI S X, DENG Y, et al. Portable T-2 toxin biosensor based on target-responsive DNA hydrogel using water column height as readout[J]. Talanta, 2024, 276:126203.
[19] WANG X M, CHEN C, WATERHOUSE G I N, et al. Ultra-sensitive detection of streptomycin in foods using a novel SERS switch sensor fabricated by AuNRs array and DNA hydrogel embedded with DNAzyme[J]. Food Chemistry, 2022, 393:133413.
[20] CHEN Q S, WU L F, ZHAO F, et al. Construction of hybridization chain reaction induced optical signal directed change of photonic crystals-DNA hydrogel sensor and its visual determination for aflatoxin B1[J]. Food Chemistry, 2023, 418:135891.
[21] LI G H, PANG W, BIAN Y L, et al. A surface-enhanced Raman scattering and colorimetric dual-mode aptasensor for ultrasensitive detection of kanamycin based on DNA hydrogel network fishing the MIL-101@AuNP nanohybrids[J]. Sensors and Actuators B: Chemical, 2024, 414:135937.
[22] UM S H, LEE J B, PARK N, et al. Enzyme-catalysed assembly of DNA hydrogel[J]. Nature Materials, 2006, 5(10):797-801.
[23] SUN Y H, LYU Y, ZHANG Y, et al. A stimuli-responsive colorimetric aptasensor based on the DNA hydrogel-coated MOF for fumonisin B1 determination in food samples[J]. Food Chemistry, 2023, 403:134242.
[24] LU J, YANG X F, XIAO J X, et al. DNA-functionalized cryogel based colorimetric biosensor for sensitive on-site detection of aflatoxin B1 in food samples[J]. Talanta, 2024, 275:126122.
[25] YANG Y, LI G L, WU D, et al. Recent advances on toxicity and determination methods of mycotoxins in foodstuffs[J]. Trends in Food Science & Technology, 2020, 96:233-252.
[26] 张琦, 孙锐, 张瑜, 等. DNA杂化水凝胶的构建及性能[J]. 高等学校化学学报, 2023, 44(4):242-248.
ZHANG Q, SUN R, ZHANG Y, et al. Construction and properties of DNA hybrid hydrogels[J]. Chemical Journal of Chinese Universities, 2023, 44(4):242-248.
[27] 徐若萱, 何金兴. 水凝胶基纳米复合材料在食品有害物电化学检测中的应用研究进展[J]. 粮油食品科技, 2023, 31(6):113-118.
XU R X, HE J X. Research progress of hydrogels-based materials and their application for electrochemical sensing of hazardous substances in foods[J]. Science and Technology of Cereals, Oils and Foods, 2023, 31(6):113-118.
[28] 何翠霞. 高效液相色谱在黄曲霉素检测中的应用[J]. 食品安全导刊, 2016(18):116.
HE C X. Application of high performance liquid chromatography in detection of aflatoxin[J]. China Food Safety Magazine, 2016(18):116.
[29] MA Y L, MAO Y, HUANG D, et al. Portable visual quantitative detection of aflatoxin B1 using a target-responsive hydrogel and a distance-readout microfluidic chip[J]. Lab on a Chip, 2016, 16(16):3097-3104.
[30] ZHAO M M, WANG P L, GUO Y J, et al. Detection of aflatoxin B1 in food samples based on target-responsive aptamer-cross-linked hydrogel using a handheld pH meter as readout[J]. Talanta, 2018, 176:34-39.
[31] ARAN G C, BAYRAÇ C. Simultaneous dual-sensing platform based on aptamer-functionalized DNA hydrogels for visual and fluorescence detection of chloramphenicol and aflatoxin M1[J]. Bioconjugate Chemistry, 2023, 34(5):922-933.
[32] 高翔, 李梅, 张立实. 赭曲霉毒素A的毒性研究进展[J]. 国外医学(卫生学分册), 2005(1):51-55.
GAO X, LI M, ZHANG L S. Research progress on toxicity of ochratoxin A[J]. Journal of Environmental Hygiene, 2005(1):51-55.
[33] TANG J, LIU L P, WANG H Y, et al. In situ generated PANI promoted flexible photoelectrochemical biosensor for ochratoxin A based on GOx-stuffed DNA hydrogel as enhancer[J]. Mikrochimica Acta, 2023, 190(3):106.
[34] FAN P F, LI Q J, ZHANG Z D, et al. A G-quadruplex-assisted target-responsive dual-mode aptasensor based on copper nanoclusters synthesized in situ in a DNA hydrogel for ultrasensitive detection of ochratoxin A[J]. Talanta, 2024, 270:125550.
[35] HAO L L, WANG W, SHEN X Q, et al. A fluorescent DNA hydrogel aptasensor based on the self-assembly of rolling circle amplification products for sensitive detection of ochratoxin A[J]. Journal of Agricultural and Food Chemistry, 2020, 68(1):369-375.
[36] HAO L L, LIU X T, XU S J, et al. A novel aptasensor based on DNA hydrogel for sensitive visual detection of ochratoxin A[J]. Mikrochimica Acta, 2021, 188(11):395.
[37] WU Q H, QIN Z H, KUCA K, et al. An update on T-2 toxin and its modified forms: Metabolism, immunotoxicity mechanism, and human exposure assessment[J]. Archives of Toxicology, 2020, 94(11):3645-3669.
[38] YANG X, LIU P L, CUI Y L, et al. Review of the reproductive toxicity of T-2 toxin[J]. Journal of Agricultural and Food Chemistry, 2020, 68(3):727-734.
[39] SUN Y F, LI S, CHEN R P, et al. Ultrasensitive and rapid detection of T-2 toxin using a target-responsive DNA hydrogel[J]. Sensors and Actuators B: Chemical, 2020, 311:127912.
[40] 王少康. 伏马菌素污染情况及其毒性研究进展[J]. 环境与职业医学, 2003, 20(2):129-131;133.
WANG S K. Progress in study on the fumonisin contamination status in foods and the toxicity of fumonisin[J]. Journal of Labour Medicine, 2003, 20(2):129-131;133.
[41] SUN Y H, QI S, DONG X Z, et al. Colorimetric aptasensor for fumonisin B1 detection based on the DNA tetrahedra-functionalized magnetic beads and DNA hydrogel-coated bimetallic MOFzyme[J]. Journal of Hazardous Materials, 2023, 443:130252.
[42] JING S Y, LIU C M, ZHENG J, et al. Toxicity of zearalenone and its nutritional intervention by natural products[J]. Food & Function, 2022, 13(20):10374-10400.
[43] ROGOWSKA A, POMASTOWSKI P, SAGANDYKOVA G, et al. Zearalenone and its metabolites: Effect on human health, metabolism and neutralisation methods[J]. Toxicon, 2019, 162:46-56.
[44] SUN Y H, QI S, DONG X Z, et al. Colorimetric aptasensor targeting zearalenone developed based on the hyaluronic Acid-DNA hydrogel and bimetallic MOFzyme[J]. Biosensors and Bioelectronics, 2022, 212:114366.
[45] 张鸿鹏, 谢刚, 李可敬, 等. 刺激响应型DNA水凝胶的设计合成及其在食品安全领域的研究进展[J]. 食品与发酵工业, 2024, 50(11):360-366.
ZHANG H P, XIE G, LI K J, et al. Design and synthesis of stimulus responsive DNA hydrogel and its research progress in the field of food safety[J]. Food and Fermentation Industries, 2024, 50(11):360-366.
[46] JIAN X T, FENG X Y, LUO Y N, et al. Development, preparation, and biomedical applications of DNA-based hydrogels[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9:661409.
[47] ZHAO Y M, HE B S, LI D Y, et al. Nanobody and CuS nanoflower-Au-based lateral flow immunoassay strip to enhance the detection of aflatoxin B1[J]. Foods, 2024, 13(12):1845.
[48] SUN X L, ZHAO X L, TANG J, et al. Development of an immunochromatographic assay for detection of aflatoxin B1 in foods[J]. Food Control, 2006, 17(4):256-262.
[49] KARAMI-OSBOO R, MIRABOLFATHI M. A novel dispersive nanomagnetic particle solid-phase extraction method to determine aflatoxins in nut and cereal samples[J]. Food Analytical Methods, 2017, 10(12):4086-4093.
[50] ZHANG M Y, YAN L Z, HUANG Q, et al. Highly sensitive simultaneous detection of major ochratoxins by an immunochromatographic assay[J]. Food Control, 2018, 84:215-220.
[51] ZHOU J M, YANG Q B, LIANG C, et al. Detection of ochratoxin A by quantum dots-based fluorescent immunochromatographic assay[J]. Analytical and Bioanalytical Chemistry, 2021, 413(1):183-192.
[52] OLIA A E A, MOHADESI A, FEIZY J. A fabric phase sorptive extraction protocol combined with liquid chromatography-fluorescence detection for the determination of ochratoxin in food samples[J]. Food Analytical Methods, 2023, 16(5):974-984.
[53] BADALI A, JAVADI A, AFSHAR MOGADDAM M R, et al. Dispersive solid phase extraction-dispersive liquid-liquid microextraction of mycotoxins from milk samples and investigating their decontamination using microwave irradiations[J]. Microchemical Journal, 2023, 190:108645.
[54] VISCONTI A, LATTANZIO V M T, PASCALE M, et al. Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection[J]. Journal of Chromatography A, 2005, 1075(1-2):151-158.
[55] PASCALE M, PANZARINI G, VISCONTI A. Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection[J]. Talanta, 2012, 89:231-236.
[56] KALTNER F, RAMPL C, RYCHLIK M, et al. Development and validation of a cost-effective HPLC-FLD method for routine analysis of fumonisins B1 and B2 in corn and corn products[J]. Food Analytical Methods, 2017, 10(5):1349-1358.
[57] DENG M, LI W Q, CHEN Y B, et al. Detection of fumonisin B1 by aptamer-functionalized magnetic beads and ultra-performance liquid chromatography[J]. Microchemical Journal, 2022, 178:107346.
[58] CHEN C C, YU X Z, HAN D G, et al. Non-CTAB synthesized gold nanorods-based immunochromatographic assay for dual color and on-site detection of aflatoxins and zearalenones in maize[J]. Food Control, 2020, 118:107418.
[59] ZHANG Y, YANG W G, SU M L, et al. A reagent-based label free electrochemiluminescence biosensor for ultrasensitive quantification of low-abundant chloramphenicol[J]. Microchemical Journal, 2024, 198:110124.
[60] MO F L, JIANG K, ZHAO D, et al. DNA hydrogel-based gene editing and drug delivery systems[J]. Advanced Drug Delivery Reviews, 2021, 168: 79-98.
[61] VÖLLMECKE K, AFROZ R, BIERBACH S, et al. Hydrogel-based biosensors[J]. Gels, 2022, 8(12): 768.
[62] WU J Y, LIYARITA B R, ZHU H S, et al. Self-assembly of dendritic DNA into a hydrogel: Application in three-dimensional cell culture[J]. ACS Applied Materials & Interfaces, 2021, 13(42):49705-49712.
[63] WEI Y H, WANG K Z, LUO S H, et al. Programmable DNA hydrogels as artificial extracellular matrix[J]. Small, 2022, 18(36): e2107640.
Outlines

/