To develop an accurate, sensitive, and specific method for the detection of aflatoxins B1(AFB1) in complex food matrix, antibody modified magnetic nano-microspheres (MMPMs-AFB1 antibody) was used as the capture probes, and antigen/GOx modified nano-microspheres (AFB1-OVA-PPs-GOx) was used as the signal probes, combining with Fenton reaction-sulfur T (ThT) fluorescent nucleic acid probe system, a “turn on” type AFB1 magnetic separation fluorescence immunoassay method was developed.The detection range of AFB1by this method was 0.05-50 ng/mL and the detection limit was 0.027 ng/mL.The concentration of the spiked sample was 1, 5, 20, 200 ng/g(ng/mL), the recovery of AFB1 in spiked foodstuffs ranged from 81.6% to 112.1%, with the coefficient of variation of 1.2%-15.8%.In quality control sample of corn flour, the method was successfully applied to detect AFB1.This method has high sensitivity, good specificity and strong anti-matrix interference ability, and can be used for accurate quantitative detection of AFB1 in food.
LI Yanan
,
HAN Aiyun
,
XIE Libin
,
ZHANG Wenna
,
WEI Meng
,
MA Yuanyuan
,
HU Lianxia
,
XIAO Xiao
. A method for determination of aflatoxin B1 in food by using thioflavine T-based nucleic acid probe cascade immunofluorescence assay[J]. Food and Fermentation Industries, 2025
, 51(2)
: 331
-337
.
DOI: 10.13995/j.cnki.11-1802/ts.039446
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