The research team isolated Bifidobacterium bifidum B1628 with good acid and bile salt tolerance from the intestine of healthy infants.Heat-killed organisms from this strain can relieve colonic inflammatory edema in inflammatory bowel disease (IBD) mice, which has potential applications.To explore the genetic background of B.bifidum B1628 and the genes with potential probiotic properties, the complete genome of B.bifidum B1628 was sequenced and assembled by combining Illumina and Oxford Nanopore.The genomes of B.bifidum were compared with those of 119 B.bifidum isolates from different countries and regions published by NCBI.The study found that B.bifidum B1628 had only one circular chromosome of 2 234 414 bp and contained no plasmid, with a GC content of 62.4% and 1 769 protein-coding regions.120 strains of B.bifidum contained a total of 6 554 pan genes in an open pattern.A phylogenetic tree was constructed based on the 987 core genes, and the 120 B.bifidum strains were clustered into eight clads with regional aggregation, and B.bifidum B1628 belonged to clade 1, which was isolated from China.Functional annotation showed that B.bifidum B1628 contained genes for folate, tryptophan, and lactate synthesis, genes for acid tolerance, bile salt tolerance, oxidative stress-related genes, and environmental stress-related genes, as well as CRISPR-Cas adaptive immune system.This study presents the genome-wide characterization of B.bifidum B1628 and identifies potential beneficial genes, providing data reference for the development, utilization, and industrialization of this strain.
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