With the rapid growth of population, the global demand for high-quality protein is increasing day by day.To alleviate the problem of protein shortage, mycelium can be used as a new source of protein through the cultivation of high-quality strains of edible fungi breeding.In this study, Pleurotus djamor GXGD-13-1 was used as the original strain, protoplasmic cell fusion technique was used to increase its protein yield.First mycelium cell walls were enzymolysised in M2 composite for 3 hours, then released protoplast was fused with polyethylene glycol (PEG) in which 250 g/L of PEG was used as the fusion agent.A high fusion rate of 53% was achieved after the process, removing the breeding barrier of low compatibility of the tetrpolarity mating system.Primary screening and re-screening of fusion strain R-1 were carried out to select a high mycelial protein yield, 23% higher than that of the original strain GXGD-13-1.The work in this paper proved the efficiency of protoplasmic somatic cell fusion breeding technology in the selection of high-yield mycelium protein strains and provided an alternative way to meet the increasing demand for protein.
WEI Hefen
,
PAN Jing
,
ZHANG Jian
,
WEN Qingshi
,
LIU Qingguo
,
TANG Chenglun
,
ZHOU Ping
. A high-yield mycelial protein strain was selected by fusion of plasma cells of Pleurotus djamor[J]. Food and Fermentation Industries, 2025
, 51(3)
: 147
-153
.
DOI: 10.13995/j.cnki.11-1802/ts.038896
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