To investigate the effect of apple vinegar powder (AVP) on functional constipation in mice and its impact on gut microbiota, this study used forty SPF BALB/c male mice.They were randomly divided into the control group, model group, low-dose apple cider vinegar model group (AVPLM), and high-dose apple cider vinegar model group (AVPHM), with ten mice in each group.After treatment of AVP, the constipation indexes of the mice were compared.The gastrointestinal active peptides and serotonin (5-HT) levels in serum were detected by ELISA, colon histopathology was observed by H&E staining, and the changes of gut microbiota were analyzed by 16S rRNA gene amplicon sequencing.Compared with the control group, the model group significantly prolonged the time of the first black stool excretion, reduced the water content of feces, and decreased the small intestine propulsion rate.After AVP intervention, the time of the first black stool excretion was significantly shortened, the water content of feces was increased, and the small intestine propulsion rate was reduced.This study also found that AVP significantly increased motilin (MTL), substance P (SP), and 5-HT while decreasing somatostatin (SS) and vasoactive intestinal peptide (VIP) in serum.Furthermore, AVP treatment increased the length of colon crypts.The alpha-diversity of the AVPLM group and the AVPHM group were both higher than that of the model group and the control group.Partial least squares discriminant analysis showed that the gut microbiota structure was similar within the group, but there were significant differences between groups.This study indicated that AVP could improve the defecation ability and diversity of gut microbiota in constipated mice, and may have the therapeutic potential in relieving constipation.
CHEN Qingqing
,
LIU Zheng
,
ZHOU Qing
,
NIE Siyu
,
DONG Yanlin
,
GENG Yan
. Relieving effect of apple vinegar powder on constipation induced by loperamide hydrochloride in mice[J]. Food and Fermentation Industries, 2025
, 51(4)
: 91
-97
.
DOI: 10.13995/j.cnki.11-1802/ts.038943
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