Food and Fermentation Industries >

2025 , Vol. 51 >Issue 4: 335 - 344

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039164

Establishment of real-time fluorescence loop-mediated isothermal amplification method for rapid detection of aflatoxin-producing fungi

  • CHEN Chen ,
  • TAO Ze ,
  • WANG Ke ,
  • LI Qingqing ,
  • AN Hongyu ,
  • LU Zenghui ,
  • LI Tuoping ,
  • LI Suhong
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  • 1(College of Food Science, Shenyang Agricultural University, Shenyang 110866, China)
    2(Central Grain Reserve Xinmin Direct Warehouse Co, Xinmin 110300, China)
    3(Central Grain Reserve Shenyang Direct Warehouse Co, Shenyang 110000, China)
    4(Liaoning Inspection, Examination & Certification Centre, Shenyang 110000, China)

Received date: 2024-03-10

  Revised date: 2024-04-15

  Online published: 2025-03-10

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Abstract

Aflatoxins are microbial toxins that are highly hepatic-inducing, teratogenic and carcinogenic.The LAMP primers were designed according to the aflatoxin-producing regulatory gene aflR, the gothramoxin transmethylase gene omt-1, and the variegated aspergilloin A dehydrogenase gene ver-1, respectively, and a real-time loop-mediated isothermal amplification (LAMP) system was established and optimized.Results showed that the detection limits of Aspergillus flavus DNA for primers designed for aflR, omt-1, and ver-1 genes were 1.0×10-2, 1.0×10-3, and 1.0×10-4 ng/μL, respectively.The system reacted positively with Aspergillus flavus and Aspergillus parasitica, but negatively with other bacteria, with the help of a thermostatic fluorescence reading device or according to the color change of the developer, it can quickly determine whether the strains to be tested are aflatoxins within 60 minutes.

Key words： aflatoxin-producing fungi; real-time fluorescent loop-mediated isothermal amplification; rapid detection; aflR; omt-1; ver-1

Cite this article

CHEN Chen , TAO Ze , WANG Ke , LI Qingqing , AN Hongyu , LU Zenghui , LI Tuoping , LI Suhong . Establishment of real-time fluorescence loop-mediated isothermal amplification method for rapid detection of aflatoxin-producing fungi[J]. Food and Fermentation Industries, 2025 , 51(4) : 335 -344 . DOI: 10.13995/j.cnki.11-1802/ts.039164

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