This study used germinated kidney beans as the raw materials for the synergy treatment of monosodium glutamate (MSG) and low-temperature freeze-thaw, the glutamate decarboxylase (GAD), diamine oxidase (DAO), polyamine oxidase (PAO) activity before and after treatment were determined, and an untargeted metabolomics analysis was used to investigate the mechanism of γ-aminobutyric acid (GABA) enrichment in germinated kidney beans with synergy treatment of MSG and low-temperature free-thaw.Results showed that the synergy treatment of MSG and low-temperature freeze-thaw could promote GABA enrichment in germinated kidney beans, in which the GABA content was 3.33 times higher than that of germinated kidney beans only.Meanwhile, the synergy treatment of MSG and low-temperature freeze-thaw had no significant effect on GAD activity in germinated kidney beans, while decreasing DAO activity and PAO activity significantly.Untargeted metabolomics studies found that the synergy treatment of MSG and low temperature freeze-thaw could change the amino acids, lipids and carbohydrate metabolites in germinated kidney beans, indicating that GABA enrichment mainly by regulating the pathways of arginine and proline metabolism, β-alanine metabolism, nicotinate and nicotinamide metabolism, alanine, aspartate and glutamate metabolism, and butanoate metabolism.
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