Food and Fermentation Industries >

2025 , Vol. 51 >Issue 6: 144 - 151

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038354

Evaluation of in vitro probiotic characteristics and safety of Lactobacillus plantarum isolated from breast milk and infant feces

  • XU Jingjing ,
  • YU Junjuan ,
  • YAN Haodong ,
  • ZHANG Chenchen ,
  • KANG Wenli ,
  • PAN Lina ,
  • WANG Jiaqi ,
  • LI Wei ,
  • GU Ruixia
Expand
  • 1(College of Food Science and Engineering, Yangzhou University, Yangzhou 225000, China)
    2(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou 225000, China)
    3(Ausnutria Dairy Corporation Ltd., Changsha 410127, China)
    4(Hunan Engineering Research Center of Human Microecological Products, Changsha 410127, China)

Received date: 2023-12-23

  Revised date: 2024-04-16

  Online published: 2025-04-14

Fold

Abstract

To screen candidate strains of probiotics for infants, lactic acid bacteria (LAB) strains were isolated from breast milk and infant feces and screened according to bile salt tolerance.Then the strains were explored by investigating acid tolerance, simulated gastrointestinal fluid tolerance, adhesion ability, bacteriostatic ability, hemolytic ability, amine production ability, and antibiotic resistance.A total of 131 strains of LAB were isolated, among which four strains showed better bile salt tolerance.The survival rates of all these four strains were higher than 45% under 0.1% bile salt, and all these strains belonged to Lactobacillus plantarum.The survival rates of these strains were higher than 50% under pH 2.5 simulated gastric juice.The survival rate of M664 under simulated gastrointestinal fluid was 49.32%.The adhesion rate of digested M678 cells to Caco-2 cells was 10.98%.The diameters of the bacteriostatic circle of the four strains to the six spoilage bacteria and pathogens were all larger than 16 mm.In addition, these strains did not produce biological amines and were sensitive to most antibiotics.Overall, L.plantarum strains obtained in this study were different from Lactobacillus isolated from breast milk and infant feces from Western dietary pattern countries.L.plantarum M664 had better gastrointestinal environmental tolerance, and L.plantarum M678 had better antibacterial ability and cell adhesion ability.These two strains showed better probiotic potential and safety and were excellent candidate strains of probiotics for Chinese infants.

Key words： breast milk; infant feces; Lactobacillus plantarum; safety evaluation

Cite this article

XU Jingjing , YU Junjuan , YAN Haodong , ZHANG Chenchen , KANG Wenli , PAN Lina , WANG Jiaqi , LI Wei , GU Ruixia . Evaluation of in vitro probiotic characteristics and safety of Lactobacillus plantarum isolated from breast milk and infant feces[J]. Food and Fermentation Industries, 2025 , 51(6) : 144 -151 . DOI: 10.13995/j.cnki.11-1802/ts.038354

References

[1] LYONS K E, ANTHONY RYAN C, DEMPSEY E M, et al.Breast milk, a source of beneficial microbes and associated benefits for infant health[J].Nutrients, 2020, 12(4):1039.
[2] 张志焱, 刘红莲, 刘海涛, 等.乳酸菌发酵上清液对肉仔鸡生产性能和免疫功能的影响[J].中国畜牧杂志, 2013, 49(15):78-82.
ZHANG Z Y, LIU H L, LIU H T, et al.Effects of fermentation supernatant of lactic acid bacteria on performance and immune function of broilers[J].Chinese Journal of Animal Science, 2013, 49(15):78-82.
[3] ŁUBIECH K, TWARUZ·EK M.Lactobacillus bacteria in breast milk[J].Nutrients, 2020, 12(12):3783.
[4] LÓPEZ-HUERTAS E.Safety and efficacy of human breast milk Lactobacillus fermentum CECT 5716.A mini-review of studies with infant formulae[J].Beneficial Microbes, 2015, 6(2):219-224.
[5] 国家卫生健康委员会. 关于发酵乳杆菌CECT5716等3个菌种的公告:卫生部公告(2016)6号[EB/OL].(2016-05-30)/[2023-12-13].https://zwfw.nhc.gov.cn/kzx/tzgg/xspylsp_225/202005/t20200526_1289.html.
[6] RIAZ RAJOKA M S, MEHWISH H M, SIDDIQ M, et al.Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk[J].LWT, 2017, 84:271-280.
[7] 国家卫生健康委员会. 关于公布可用于婴幼儿食品的菌种名单的公告:卫生部公告(2011)25号[EB/OL].(2011-12-24)/[2023-12-13].http://www.nhc.gov.cn/sps/s7891/201111/a10fe4a0b1dd477c9884649220368cc 2.shtml.
[8] 国家卫生健康委员会. 关于β-1,3/α-1,3-葡聚糖等6种“三新食品”的公告:卫生部公告(2021)5号[EB/OL].(2021-04-15)/[2023-12-13].https://zwfw.nhc.gov.cn/kzx/tzgg/xspylsp_225/202104/t20210426_2094.html.
[9] 胡鹏钰, 于俊娟, 王鹏, 等.母乳来源益生菌的筛选及潜在益生特性研究[J].食品与发酵工业, 2021, 47(14):190-195.
HU P Y, YU J J, WANG P, et al.Screening of probiotics from breast milk and its potential probiotic characteristics[J].Food and Fermentation Industries, 2021, 47(14):190-195.
[10] SAITOU N, NEI M.The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution, 1987, 4(4):406-425.
[11] 陈大卫. 辅助降血脂益生乳酸菌的筛选及其对高血脂大鼠肠道菌群的影响[D].扬州:扬州大学, 2015.
CHEN D W.Screening of probiotic lactic acid bacteria to help reduce blood lipid and its effect on intestinal flora in hyperlipidemia rats[D].Yangzhou:Yangzhou University, 2015.
[12] LIU W J, CHEN M X, DUO L N, et al.Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum[J].Journal of Dairy Science, 2020, 103(5):4013-4025.
[13] 陈春萌. 消化应激对乳酸菌黏附能力的影响[D].扬州:扬州大学, 2021.
CHEN C M.Effect of digestive stress on adhesion of lactic acid bacteria[D].Yangzhou:Yangzhou University, 2021.
[14] Clinical and Laboratory Standards Institute (CLSI).Performance standards for antimicrobial susceptibility testing[S].Wayne:CLSI, 2017:64-67.
[15] 安浩然. 长双歧杆菌BBMN68抗胆盐胁迫反应机制及双组分系统在胆盐胁迫应答中的作用[D].北京:中国农业大学, 2014.
AN H R.Mechanism of Bifidobacterium longum BBMN68' s resistance to bile salt stress and the role of two-component system in bile salt stress response[D].Beijing:China Agricultural University, 2014.
[16] KALANTZI L, GOUMAS K, KALIORAS V, et al.Characterization of the human upper gastrointestinal contents under conditions simulating bioavailability/bioequivalence studies[J].Pharmaceutical Research, 2006, 23(1):165-176.
[17] GLASGOW J F, DINSMORE H, MOLLA A, et al.A comprehensive study of duodenal bile salts in newborn infants and their relationship to fat absorption[J].Irish Journal of Medical Science, 1980, 149(9):346-356.
[18] JÄRVENPÄÄ A L, RASSIN D K, KUITUNEN P, et al.Feeding the low-birth-weight infant.III.Diet influences bile acid metabolism[J].Pediatrics, 1983, 72(5):677-683.
[19] LUZ C, CALPE J, MANUEL QUILES J, et al.Probiotic characterization of Lactobacillus strains isolated from breast milk and employment for the elaboration of a fermented milk product[J].Journal of Functional Foods, 2021, 84:104599.
[20] JARA S, SÁNCHEZ M, VERA R, et al.The inhibitory activity of Lactobacillus spp.isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin[J].Anaerobe, 2011, 17(6):474-477.
[21] DUBOS C, VEGA N, CARVALLO C, et al.Identification of Lactobacillus spp.in colostrum from Chilean mothers[J].Archivos Latinoamericanos de Nutricion, 2011, 61(1):66-68.
[22] JAMYUANG C, PHOONLAPDACHA P, CHONGVIRIYAPHAN N, et al.Characterization and probiotic properties of Lactobacilli from human breast milk[J].3 Biotech, 2019, 9(11):398.
[23] SHARMA C, SINGH B P, THAKUR N, et al.Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens[J].3 Biotech, 2017, 7(1):31.
[24] MATIJAŠIĆ B B, TUŠAR T, BRATANIČ B, et al.Cultivable bacteria from milk from Slovenian breastfeeding mothers[J].Food Technology and Biotechnology, 2014, 52(2):242-247.
[25] KOZAK K, CHARBONNEAU D, SANOZKY-DAWES R, et al.Characterization of bacterial isolates from the microbiota of mothers' breast milk and their infants[J].Gut Microbes, 2015, 6(6):341-351.
[26] HAARMAN M, KNOL J.Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula[J].Applied and Environmental Microbiology, 2006, 72(4):2359-2365.
[27] GARCIA-GUTIERREZ E, O’CONNOR P M, COLQUHOUN I J, et al.Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk[J].Applied Microbiology and Biotechnology, 2020, 104(9):3869-3884.
[28] SOTO A, MARTÍN V, JIMÉNEZ E, et al.Lactobacilli and bifidobacteria in human breast milk:Influence of antibiotherapy and other host and clinical factors[J].Journal of Pediatric Gastroenterology and Nutrition, 2014, 59(1):78-88.
[29] ROMAN C, CARRIERE F, VILLENEUVE P, et al.Quantitative and qualitative study of gastric lipolysis in premature infants:Do MCT-enriched infant formulas improve fat digestion?[J].Pediatric Research, 2007, 61(1):83-88.
[30] 高盛, 乔宇, 张宇微, 等.人母乳源乳酸菌的筛选、鉴定及益生活性的初步研究[J].食品工业科技, 2017, 38(10):205-210.
GAO S, QIAO Y, ZHANG Y W, et al.Isolation, identification and probiotic characterization of lactic acid bacteria in human breast milk[J].Science and Technology of Food Industry, 2017, 38(10):205-210.
[31] KANG W L, PAN L N, PENG C, et al.Isolation and characterization of lactic acid bacteria from human milk[J].Journal of Dairy Science, 2020, 103(11):9980-9991.
[32] 罗强, 张明, 刘巧, 等.产细菌素屎肠球菌SC-Y112的体外益生性及安全性评价[J].食品科学, 2021, 42(11):154-160.
LUO Q, ZHANG M, LIU Q, et al.Evaluation of in vitro probiotic and safety properties of Enterococcus faecium SC-Y112 producing bacteriocin[J].Food Science, 2021, 42(11):154-160.
[33] WU C D, HE G Q, ZHANG J.Physiological and proteomic analysis of Lactobacillus casei in response to acid adaptation[J].Journal of Industrial Microbiology & Biotechnology, 2014, 41(10):1533-1540.
[34] 尹春媚. 母乳源乳酸菌的筛选、鉴定及其益生特性研究[D].长春:吉林农业大学, 2020.
YIN C M.Screening, identification and probiotic characteristics of lactic acid bacteria from breast milk[D].Changchun:Jilin Agricultural University, 2020.
[35] 喻玲, 许柳, 才丽娜, 等.凝血功能、血乳酸与新生儿ABO溶血病相关性研究[J].华北理工大学学报(医学版), 2021, 23(4):258-261;267.
YU L, XU L, CAI L N, et al.Correlation between coagulation function, blood lactic acid and neonatal ABO hemolytic disease[J].Journal of North China University of Science and Technology (Health Sciences Edition), 2021, 23(4):258-261;267.
[36] 杨埔. 食源性乳酸菌的安全性评价[D].济南:山东大学, 2013.
YANG P.Safety evaluation of food-borne lactic acid bacteria[D].Jinan:Shandong University, 2013.
[37] SALIBA L, ZOUMPOPOULOU G, ANASTASIOU R, et al.Probiotic and safety assessment of Lactobacillus strains isolated from Lebanese Baladi goat milk[J].International Dairy Journal, 2021, 120:105092.
[38] OUWEHAND A C, FORSSTEN S, HIBBERD A A, et al.Probiotic approach to prevent antibiotic resistance[J].Annals of Medicine, 2016, 48(4):246-255.
[39] JI J F, YANG H.Using probiotics as supplementation for Helicobacter pylori antibiotic therapy[J].International Journal of Molecular Sciences, 2020, 21(3):1136.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.