The process of extracting pectin from pomelo peel using low-temperature plasma technology was optimized through single-factor and orthogonal experiments, and its monosaccharide composition, thermal stability, emulsification properties, rheological characteristics, and antioxidant activity were characterized.Results showed that the optimal extraction conditions for pomelo peel rhamnus galacturonide Ⅰ (RG-Ⅰ) pectin were temperature of 65 ℃, dielectric barrier discharge (DBD) treatment time of 15 min, and sodium hydroxide concentration of 2.2 g/L.Under these conditions, the extraction rate of pomelo peel RG-Ⅰ pectin was (24.85±0.64)%.The monosaccharide composition of the obtained pectin was in the order of galacturonic acid, arabinose, galactose, xylose, glucose, mannose, and rhamnose, with the lowest degree of linearization of the pectin molecules and the highest content of RG-Ⅰ structure at (61.14±0.09) mol%, along with the highest degree of side chain branching.Regarding thermal stability, both the pectin treated with DBD (DAP) and the control sample (AP) showed better thermal stability than commercial pectin (CP).DAP exhibited the best emulsification performance, with slightly lower emulsification stability than CP but higher than AP.Rheological studies indicated that AP, DAP, and CP were shear-thinning non-pseudoplastic fluids, with CP having higher apparent viscosity than AP and DAP.In vitro antioxidant experiments showed that DAP had the highest ABTS cationic radical scavenging rate and oxygen free radical absorption capacity.
LAI Huining
,
ZHAO Zhengang
,
XU Yujuan
,
WU Jijun
,
KANG Zhiying
,
YU Yuanshan
,
WANG Yanhui
,
HUANG Wenqian
,
WEN Jing
,
LIU Haiyang
. Extraction and characterization of rhamnus galacturonide Ⅰ pectin from pomelo peel by low temperature plasma technology[J]. Food and Fermentation Industries, 2025
, 51(6)
: 183
-192
.
DOI: 10.13995/j.cnki.11-1802/ts.039303
[1] 高歌. 超高压技术在红柚汁加工与柚皮果胶提取中应用研究[D].北京:中国农业大学, 2018.
GAO G. Study on the application of ultra-high pressure technology in the processing of red pomelo juice and the extraction of pectin from pomelo peel[D]. Beijing: China Agricultural University, 2018.
[2] CUI J F, ZHAO C Y, FENG L P, et al.Pectins from fruits:Relationships between extraction methods, structural characteristics, and functional properties[J].Trends in Food Science & Technology, 2021, 110:39-54.
[3] WU D M, ZHENG J Q, MAO G Z, et al.Rethinking the impact of RG-I mainly from fruits and vegetables on dietary health[J].Critical Reviews in Food Science and Nutrition, 2020, 60(17):2938-2960.
[4] WANG J, XING C R, XIA J, et al.Degradation of carbendazim in aqueous solution by dielectric barrier discharge cold plasma:Identification and toxicity of degradation products[J].Food Chemistry, 2023, 403:134329.
[5] WANG Z W, JIA H, YANG J Y, et al.Inactivation of Alicyclobacillus contaminans in apple juice by dielectric barrier discharge plasma[J].Food Control, 2023, 146:109475.
[6] XI J, WANG Y, ZHOU X B, et al.Cold plasma pretreatment technology for enhancing the extraction of bioactive ingredients from plant materials:A review[J].Industrial Crops and Products, 2024, 209:117963.
[7] HEYDARI M, CARBONE K, GERVASI F, et al.Cold plasma-assisted extraction of phytochemicals:A review[J].Foods, 2023, 12(17):3181.
[8] 张颂. 介质阻挡放电等离子体对柑橘果胶的提取及降解研究[D].烟台:烟台大学, 2018.
ZHANG S.Study on extraction and degradation of citrus pectin by dielectric barrier discharge plasma[D].Yantai:Yantai University, 2018.
[9] 许涵婷, 唐语谦, 胡腾根, 等.荔枝果渣可溶性膳食纤维去结合酚前后结构和功能性质的比较[J].现代食品科技, 2023, 39(8):206-212.
XU H T, TANG Y Q, HU T G, et al.Comparison of the structure and functional properties of soluble dietary fiber from lychee pomace before and after the removal of bound phenolics[J].Modern Food Science and Technology, 2023, 39(8):206-212.
[10] 胡薇薇. 声压联合辅助提取Ⅰ型鼠李半乳糖醛酸(RG-Ⅰ)果胶多糖机制研究[D].杭州:浙江大学, 2021.
HU W W. Study on the mechanism of extraction of pectin polysaccharide from rhamnuronic acid type Ⅰ (RG-Ⅰ) by combined sound pressure[D]. Hangzhou: Zhejiang University, 2021.
[11] 杨涛. 豆渣转化为食品级高效皮克林稳定剂的途径及机理[D].广州:华南理工大学, 2021.
YANG T. Ways and mechanism of transforming bean dregs into food-grade efficient Pickering stabilizer[D]. Guangzhou: South China University of Technology, 2020.
[12] 徐维盛, 李东, 刘静, 等. ORAC法对12种水果总抗氧化能力评价研究[J]. 食品工业, 2014, 35(1): 247-250.
XU W S, LI D, LIU J, et al. Study on antioxidant activities of 12 kinds of fruit[J]. The Food Industry, 2014, 35(1): 247-250.
[13] WANDEE Y, UTTAPAP D, MISCHNICK P.Yield and structural composition of pomelo peel pectins extracted under acidic and alkaline conditions[J].Food Hydrocolloids, 2019, 87:237-244.
[14] 黄莹星. 柠檬皮高酯果胶的分步提取及其性能研究[D].广州:华南理工大学, 2020.
HUANG Y X. Step-by-step extraction of high-ester pectin from lemon peel and its properties[D]. Guangzhou: South China University of Technology, 2020.
[15] BROXTERMAN S E, SCHOLS H A.Interactions between pectin and cellulose in primary plant cell walls[J].Carbohydrate Polymers, 2018, 192:263-272.
[16] DRANCA F, VARGAS M, OROIAN M.Physicochemical properties of pectin from Malus domestica ‘Faălticeni’apple pomace as affected by non-conventional extraction techniques[J].Food Hydrocolloids, 2020, 100:105383.
[17] GUO Z L, WU S Z, ZHENG H, et al.Effects of dielectric barrier discharge plasma modification on structure, physicochemical and functional properties of watermelon peel pectin[J].Food Hydrocolloids, 2024,147:109350.
[18] HU W W, YE X Q, CHANTAPAKUL T, et al.Manosonication extraction of RG-Ⅰ pectic polysaccharides from citrus waste:Optimization and kinetics analysis[J].Carbohydrate Polymers, 2020, 235:115982.
[19] KESHAVARZI M, NAJAFI G, GAVLIGHI H A, et al.Enhancement of polyphenolic content extraction rate with maximal antioxidant activity from green tea leaves by cold plasma[J].Journal of Food Science, 2020, 85(10):3415-3422.
[20] MELOTTI L, MARTINELLO T, PERAZZI A, et al.Could cold plasma act synergistically with allogeneic mesenchymal stem cells to improve wound skin regeneration in a large size animal model?[J].Research in Veterinary Science, 2021, 136:97-110.
[21] BURSAĆ KOVAČEVIĆ D, MARAS M, BARBA F J, et al. Innovative technologies for the recovery of phytochemicals from Stevia rebaudiana Bertoni leaves:A review[J].Food Chemistry, 2018, 268:513-521.
[22] KARUNANITHI S, GUHA P, SRIVASTAV P P.Cold plasma-assisted microwave pretreatment on essential oil extraction from betel leaves:process optimization and its quality[J].Food and Bioprocess Technology, 2023, 16(3):603-626.
[23] ZHAO J Y, HONG T, HOU Y J, et al.Comparison of structures and emulsifying properties between water-extracted pectins from Fructus aurantii[J].International Journal of Biological Macromolecules, 2023, 242:125005.
[24] XU F Z, ZHANG S K, WATERHOUSE G I N, et al.Yeast fermentation of apple and grape pomaces affects subsequent aqueous pectin extraction:Composition, structure, functional and antioxidant properties of pectins[J].Food Hydrocolloids, 2022, 133:107945.
[25] QI X D, YU Y, WANG X Y, et al.Structural characterization and anti-oxidation activity evaluation of pectin from Lonicera japonica Thunb[J].Frontiers in Nutrition, 2022, 9:998462.