Preparation of bitter almond polypeptide/chitosan composite film and its preservation effect on cheese

  • ZHANG Tao ,
  • ZHOU Zhiyi ,
  • DENG Wenqi ,
  • ZHANG Ting ,
  • MA Jie ,
  • WANG Yinuo ,
  • YU Junfei ,
  • ZHOU Jianzhong
Expand
  • (College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830000, China)

Received date: 2024-03-08

  Revised date: 2024-04-12

  Online published: 2025-04-14

Abstract

To explore the improvement of the physical and chemical structure and bacteriostatic efficacy of chitosan composite film by adding bitter almond antibacterial peptide and to expand the application of bitter almond antibacterial peptide in the field of food preservation, the bitter almond peptide/chitosan degradable composite film was prepared with chitosan and gelatin as film-forming agents, and the cheese was wrapped.The effect of the additional amount of bitter almond antibacterial peptide on the properties of the chitosan composite film was investigated, and the physical properties, antibacterial activity, and fresh-keeping effect of the composite film on cheese were studied.Results showed that adding bitter almond antibacterial peptide could enhance the antibacterial activity of chitosan film, improve the opacity of the film, reduce water vapor permeability, and maintain elongation at break and tensile strength.Meanwhile, the bitter almond polypeptide/chitosan composite film could more effectively inhibit the growth of microorganisms, prolong the shelf life of cheese, maintain its original color, flavor, and quality, and have the potential to be further developed into food preservation packaging.This study demonstrates that the bitter almond polypeptide/chitosan composite membrane plays an important role in protecting the quality of cheese, which provides a theoretical basis for improving the quality stability of cheese products.

Cite this article

ZHANG Tao , ZHOU Zhiyi , DENG Wenqi , ZHANG Ting , MA Jie , WANG Yinuo , YU Junfei , ZHOU Jianzhong . Preparation of bitter almond polypeptide/chitosan composite film and its preservation effect on cheese[J]. Food and Fermentation Industries, 2025 , 51(6) : 232 -238 . DOI: 10.13995/j.cnki.11-1802/ts.039148

References

[1] 蓝敏怡, 李会茹, 胡立新, 等.塑料食品包装材料的环境污染综述[J].生态毒理学报, 2021, 16(5):186-210.
LAN M Y, LI H R, HU L X, et al.A review of the environmental pollution of food plastic packaging materials[J].Asian Journal of Ecotoxicology, 2021, 16(5):186-210.
[2] LIU E K, HE W Q, YAN C R.‘White revolution’ to ‘white pollution’:Agricultural plastic film mulch in China[J].Environmental Research Letters, 2014, 9(9):091001.
[3] 张亚美, 池帅, 李颖畅, 等.生物保鲜剂在水产品保鲜中的应用及研究进展[J].中国食品添加剂, 2023,34 (11):36-44.
ZHANG Y M, CHI S, LI Y C, et al.Application and research progress of biological preservatives in aquatic products preservation[J].China Industrial Economics, 2023,34 (11):36-44.
[4] MALIKI S, SHARMA G, KUMAR A, et al.Chitosan as a tool for sustainable development:A mini review[J].Polymers, 2022, 14(7):1475.
[5] ZOU F L, TAN C M, SHINALI T S, et al.Plant antimicrobial peptides:A comprehensive review of their classification, production, mode of action, functions, applications, and challenges[J].Food & Function, 2023, 14(12):5492-5515.
[6] WANG C, HONG T T, CUI P F, et al.Antimicrobial peptides towards clinical application:Delivery and formulation[J].Advanced Drug Delivery Reviews, 2021, 175:113818.
[7] 胡瀚文, 余雪健, 李旭, 等.壳聚糖/乳酸链球菌素复合抗菌膜的制备及其性能[J].食品与发酵工业, 2023, 49(11):13-19.
HU H W, YU X J, LI X, et al.Preparation and properties of chitosan/nisin composite antibacterial film[J].Food and Fermentation Industries, 2023, 49(11):13-19.
[8] 万怡格, 李铭贵, 郭思敏, 等.壳聚糖用于可降解食品保鲜膜的制备的可行性分析[J].中国包装, 2023, 43(3):22-25.
WAN Y G, LI M G, GUO S M, et al.Feasibility analysis of chitosan used in the preparation of degradable food preservative film[J].China Packaging, 2023, 43(3):22-25.
[9] LUZ C, CALPE J, SALADINO F, et al.Antimicrobial packaging based on ε-polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread[J].Journal of Food Processing and Preservation, 2018, 42(1):e13370.
[10] 杨续金, 范贵生.奶酪的市场发展趋势[J].农产品加工, 2006(11):57-58.
YANG X J, FAN G S.Market development trend of cheese[J].Farm Products Processing, 2006(11):57-58.
[11] DOS SANTOS GOUVEA F, ROSENTHAL A, DA ROCHA FERREIRA E H.Plant extract and essential oils added as antimicrobials to cheeses:A review[J].Ciência Rural, 2017, 47(8).DOI:10.1590/0103-8478cr20160908.
[12] 朱盼盼, 剧柠, 张书文, 等.常温奶酪加工技术及货架期稳定性研究进展[J].中国乳品工业, 2022, 50(1):32-36.
ZHU P P, JU N, ZHANG S W, et al.Advances in processing technology and shelf life stability of room temperature cheese[J].China Dairy Industry, 2022, 50(1):32-36.
[13] 韦剑思, 林莹, 韦剑欢, 等.发酵乳制品及其保鲜技术的研究进展[J].轻工科技, 2021, 37(10):12-15.
WEI J S, LIN Y, WEI J H, et al.Research progress of fermented dairy products and their preservation technology[J].Light Industry Science and Technology, 2021, 37(10):12-15.
[14] 张国琛, 徐振方, 潘澜澜.微波真空干燥技术在食品工业中的应用与展望[J].大连水产学院学报, 2004, 19(4):292-296.
ZHANG G C, XU Z F, PAN L L.The development and application of microwave vacuum drying technology in food industry[J].Journal of Dalian Fisheries University, 2004, 19(4):292-296.
[15] 张涛, 马杰, 年国芳, 等.苦杏仁抑菌肽的筛选富集及结构鉴定[J].食品工业科技, 2024,45 (10):66-76.
ZHANG T, MA J, NIAN G F, et al.Screening, enrichment and structural identification of antibacterial peptides from bitter almond[J].Science and Technology of Food Industry, 2024,45 (10):66-76.
[16] 薛蕾, 李大文, 尉芹, 等.苦杏仁蛋白的功能特性[J].食品科学, 2013, 34(7):70-75.
XUE L, LI D W, WEI Q, et al.Functional properties of bitter almond protein[J].Food Science, 2013, 34(7):70-75.
[17] 李桂峰, 赵国建, 肖春玲, 等.金针菇蛋白酶解制备抑菌肽的工艺优化及功能评价[J].中国食品学报, 2012, 12(10):107-112.
LI G F, ZHAO G J, XIAO C L, et al.Optimization of enzymolysis technology of Flammulina velutipes proteins and evaluation of peptides antibacterial activity[J].Journal of Chinese Institute of Food Science and Technology, 2012, 12(10):107-112.
[18] 董晶, 满都拉, 孙子羽, 等.壳聚糖-抗菌肽抑菌膜的制备及其在再制干酪贮藏保鲜中的应用[J].食品与发酵工业, 2022, 48(21):133-139.
DONG J, MAN D L, SUN Z Y, et al.Preparation of chitosan-antimicrobial peptides antimicrobial composite film and its application in the packaging of cheese[J].Food and Fermentation Industries, 2022, 48(21):133-139.
[19] 张海霞, 包良.紫色马铃薯花青素的抑菌性及其对草莓保鲜效果的研究[J].食品工业科技, 2021, 42(24):293-298.
ZHANG H X, BAO L.Study on bacteriostasis of purple potato anthocyanin and its fresh-keeping effect on strawberry[J].Science and Technology of Food Industry, 2021, 42(24):293-298.
[20] 付东升. 生物可降解抗菌复合薄膜的制备及其在食品保鲜中的应用研究[D].太原:太原理工大学, 2022.
FU D S.Preparation of biodegradable antibacterial composite film and its application in food preservation[D].Taiyuan:Taiyuan University of Technology, 2022.
[21] 胡寅轩, 王博莹, LEE Sungje, 等.牛油果对奶油奶酪储存期间理化性质和稳定性的影响[J].云南农业大学学报(自然科学), 2023, 38(6):1006-1014.
HU Y X, WANG B Y, SUNGJE L, et al.Effects of avocado on the physicochemical properies and stability of cream cheeses during storage[J].Journal of Yunnan Agricultural University (Natural Science), 2023, 38(6):1006-1014.
[22] 吴涵清, 洪青, 刘振民, 等.抗氧化乳酸菌的筛选及其对夸克奶酪品质的影响[J].食品与发酵工业, 2024,50 (22):286-293.
WU H Q, HONG Q, LIU Z M, et al.Screening of antioxidant lactic acid bacteria and its effect on the quality of quark cheese[J].Food and Fermentation Industries, 2024,50 (22):286-293.
[23] 段星星. Lactobacillus paracasei FX-6产抗菌肽对鸡肉的防腐效果及其抑菌机理的研究[D].广州:华南农业大学, 2019.
DUAN X X.Study on antiseptic effect and antibacterial mechanism of antibacterial peptide produced by Lactobacillus paracasei FX-6 on chicken[D].Guangzhou:South China Agricultural University, 2019.
[24] 陈欢鑫. 乳酸链球菌素-燕麦蛋白-多糖复合保鲜膜的超声波制备技术研究[D].镇江:江苏大学, 2021.
CHEN H X.Study on ultrasonic preparation technology of nisin-oat protein-polysaccharide composite preservative film[D].Zhenjiang:Jiangsu University, 2021.
[25] 肖力源, 余国贤, 雷晏琳, 等.玉米淀粉/壳聚糖/魔芋葡甘露聚糖复合膜的制备及物性研究[J].中国粮油学报, 2018, 33(4):86-92;100.
XIAO L Y, YU G X, LEI Y L, et al.Preparation and physical properties of corn/chitosan/konjac glucomannan composite film[J].Journal of the Chinese Cereals and Oils Association, 2018, 33(4):86-92;100.
[26] 麦仁标. 基于多针静电纺丝的速溶型纳米纤维面膜的制备研究[D].广州:广东工业大学, 2022.
MAI R B.Study on preparation of instant nanofiber mask based on multi-needle electrostatic spinning[D].Guangzhou:Guangdong University of Technology, 2022.
[27] 李建全, 李瑶, 王乐天, 等.磺化共价有机框架改性壳聚糖质子交换膜的制备及其性能研究[J].应用化工, 2024,53 (4):808-813.
LI J Q, LI Y, WANG L T, et al.Preparation and properties of sulfonated covalent organic framework modified chitosan proton exchange membrane[J].Applied Chemical Industry, 2024,53 (4):808-813.
[28] 李金钰. 钙盐交联植物乳杆菌培养物纤维膜的制备及其在蒙古奶酪保鲜中的应用[D].呼和浩特:内蒙古农业大学, 2022.
LI J Y.Preparation of calcium salt cross-linked Lactobacillus plantarum culture fiber membrane and its application in Mongolian cheese preservation[D].Hohhot:Inner Mongolia Agricultural University, 2022.
[29] ESPITIA P J P, DU W X, DE JESÚS AVENA-BUSTILLOS R, et al.Edible films from pectin:Physical-mechanical and antimicrobial properties—A review[J].Food Hydrocolloids, 2014, 35:287-296.
[30] KOIDE S S.Chitin-chitosan:Properties, benefits and risks[J].Nutrition Research, 1998, 18(6):1091-1101.
[31] AZMANA M, MAHMOOD S, HILLES A R, et al.A review on chitosan and chitosan-based bionanocomposites:Promising material for combatting global issues and its applications[J].International Journal of Biological Macromolecules, 2021, 185:832-848.
[32] 苏安伟. PCL/PBAT可降解膜对香蕉保鲜性能研究[J].印刷与数字媒体技术研究, 2023(6):91-98.
SU A W.Study on the preservation performance of PCL/PBAT degradable film for banana[J].Printing and Digital Media Technology Study, 2023(6):91-98.
[33] 白雪. 大豆分离蛋白/海藻酸钠复合膜的制备及在运动蛋白粉中的应用[J].中国食品添加剂, 2024, 35(3):213-219.
BAI X.Preparation of soy protein isolate/sodium alginate composite films and their application in protein powder for exercise[J].China Food Additives, 2024, 35(3):213-219.
[34] NYANGENA D N, MUTUNGI C, IMATHIU S, et al.Effects of traditional processing techniques on the nutritional and microbiological quality of four edible insect species used for food and feed in east Africa[J].Foods, 2020, 9(5):574.
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