Food and Fermentation Industries >

2025 , Vol. 51 >Issue 6: 294 - 299

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040162

Electrochemical biosensor based on aptamer-capped mesoporous material for sulfamethazine in porks

  • GUO Ting ,
  • HUANG Xinrui ,
  • ZHOU Ying ,
  • ZHANG Yuhao ,
  • LIU Xiaozhu ,
  • MA Liang
Expand
  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chongqing Key Laboratory of Specialty Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China)
    3(Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 401121, China)
    4(Foshan Micro Miracles Biotechnology Company, Foshan 528000, China)

Received date: 2024-06-12

  Revised date: 2024-07-11

  Online published: 2025-04-14

Fold

Abstract

Sulfamethazine (SMZ) as an important representative of sulfonamides is widely used in animal-derived food due to its advantages of broad spectrum and strong sterilizing ability, and it has become one of the keys of market supervision in China.Therefore, in this study, the electrochemical biosensor was constructed based on aptamers capping the mesoporous material for the detection of SMZ.In the biosensor, SMZ-specific aptamer was chosen as the target recognition probe, methylene blue (MB) as the signal enhancement probe, and mesoporous silica nanospheres (MSN) as the carrier of the electrochemical signal enhancement probe.In the presence of the SMZ, the release of MB in MSN was achieved through the conformational change due to the recognition of the aptamer and SMZ.MB promoted the electron transfer, resulting in the increase of current intensity.The quantitative determination of SMZ was conducted based on the correlation between current intensity and target concentration.Results showed that a good linear relationship between the incremental current and the logarithm of SMZ concentration had been exhibited in the range of 5 pg/mL to 10 ng/mL.This method had a high sensitivity with a detection limit of 1.024 pg/mL, and the percentage of spiked recoveries in pork samples was 98.3%-100.2%.The electrochemical biosensor had advantages such as rapidity, sensitivity, and convenience, providing essential support and foundation for the development of on-site rapid detection and screening technology in the market supervision process.

Key words： sulfamethazine; aptamer; mesoporous silica nanospheres; electrochemistry; label-free

Cite this article

GUO Ting , HUANG Xinrui , ZHOU Ying , ZHANG Yuhao , LIU Xiaozhu , MA Liang . Electrochemical biosensor based on aptamer-capped mesoporous material for sulfamethazine in porks[J]. Food and Fermentation Industries, 2025 , 51(6) : 294 -299 . DOI: 10.13995/j.cnki.11-1802/ts.040162

References

[1] 李雅琼, 耿健强, 穆同娜, 等.基于我国动物性食品中禁限用兽药使用规定的食源性兴奋剂种类浅析[J].食品科学, 2022, 43(13):319-326.
LI Y Q, GENG J Q, MU T N, et al.Analysis of food-derived doping based on the regulations on the use of veterinary drugs in animal-derived foods in China[J].Food Science, 2022, 43(13):319-326.
[2] 张元, 李伟青, 周伟娥, 等.食品中磺胺类药物前处理及检测方法研究进展[J].食品科学, 2015, 36(23):340-346.
ZHANG Y, LI W Q, ZHOU W E, et al.Progress in sample pretreatment and analytical techniques for the determination of sulfonamide residues in foods[J].Food Science, 2015, 36(23):340-346.
[3] OVUNG A, BHATTACHARYYA J.Sulfonamide drugs:Structure, antibacterial property, toxicity, and biophysical interactions[J].Biophysical Reviews, 2021, 13(2):259-272.
[4] 王冉, 刘铁铮, 耿志明, 等.兽药磺胺二甲嘧啶在土壤中的生态行为[J].土壤学报,2007,44(2):307-311.
WANG R, LIU T Z, GENG Z M, et al.Ecotoxicology and ecological behavior of sulfamethazine in soil[J].Acta Pedologica Sinica, 2007, 44(2):307-311.
[5] 常志强, 李健, 刘萍.磺胺二甲嘧啶的危害特征[J].中国渔业质量与标准,2012,2(4):15-20.
CHANG Z Q, LI J, LIU P.Toxicological character of sulfadimidine[J].Chinese Fishery Quality and Standards, 2012, 2(4):15-20.
[6] 马宁,王杰,裴斐,等.屠宰、预冷和市售阶段猪肉及内脏中兽药残留分析与风险评估[J].食品科学,2020,41(16):314-319.
MA N, WANG J, PEI F, et al.Determination and risk assessment of veterinary drugs in pork meat and viscera during slaughter, pre-cooling and sale[J].Food Science, 2020, 41(16):314-319.
[7] LIU X W, LV K, DENG C X, et al.Persistence and migration of tetracycline, sulfonamide, fluoroquinolone, and macrolide antibiotics in streams using a simulated hydrodynamic system[J].Environmental Pollution, 2019, 252:1532-1538.
[8] 黄华, 谢文东, 谷雨, 等.UPLC-MS-MS法同时测定鸡肉食品中37种兽药残留[J].食品与发酵工业, 2022, 48(13):290-296.
HUANG H, XIE W D, GU Y, et al.Determination of 37 veterinary residues in chicken by QuEChERS-UPLC-MS-MS[J].Food and Fermentation Industries, 2022, 48(13):290-296.
[9] ZHAO X L, WANG J Y, WANG J P, et al.Development of water-compatible molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry for the detection of six sulfonamides in animal-derived foods[J].Journal of Chromatography A, 2018, 1574:9-17.
[10] SU S F, ZHANG M, LI B L, et al.HPLC determination of sulfamethazine in milk using surface-imprinted silica synthesized with iniferter technique[J].Talanta, 2008, 76(5):1141-1146.
[11] EBRAHIMPOUR B, YAMINI Y, REZAZADEH M.A sensitive emulsification liquid phase microextraction coupled with on-line phase separation followed by HPLC for trace determination of sulfonamides in water samples[J].Environmental Monitoring and Assessment, 2015, 187(1):4162.
[12] 曾慧君,付诗慧,晏涛, 等.核酸适配体生物传感器在食源性致病菌检测中的应用[J].食品与发酵工业, 2020, 46(17):277-284.
ZENG H J, FU S H, YAN T, et al.Research advances of oligonucleotide aptamer-based biosensor for foodborne pathogen detection[J].Food and Fermentation Industries, 2020, 46(17):277-284.
[13] 张灵丽, 吴巧灵, 刘丰, 等.纳米材料增敏的电化学检测技术在食品双酚类物质检测中的应用进展[J].食品与发酵工业, 2021, 47(19):314-322.
ZHANG L L, WU Q L, LIU F, et al.Application progress of nano-materials sensitized electrochemical sensors in the detection of food bisphenols[J].Food and Fermentation Industries, 2021, 47(19):314-322.
[14] ZHANG Y, DUAN B, BAO Q, et al.Aptamer-modified sensitive nanobiosensors for the specific detection of antibiotics[J].Journal of Materials Chemistry. B, 2020, 8(37):8607-8613.
[15] CHEN M, GAN N, ZHOU Y, et al.A novel aptamer-metal ions-nanoscale MOF based electrochemical biocodes for multiple antibiotics detection and signal amplification[J].Sensors and Actuators B: Chemical, 2017, 242:1201-1209.
[16] MALECKA-BATURO K, ZAGANIARIS A, GRABOWSKA I, et al.Electrochemical biosensor designed to distinguish tetracyclines derivatives by ssDNA aptamer labelled with ferrocene[J].International Journal of Molecular Sciences, 2022, 23(22):13785.
[17] SUI C J, ZHOU Y L, WANG M Y, et al.Aptamer-based photoelectrochemical biosensor for antibiotic detection using ferrocene modified DNA as both aptamer and electron donor[J].Sensors and Actuators B:Chemical, 2018, 266:514-521.
[18] GAO X L, SUN Z C, WANG X Y, et al.Construction of a ratiometric electrochemical aptasensor based on graphdiyne-methylene blue and Fc-labeled hairpin for cyclic signal amplification detection of kanamycin[J].Sensors and Actuators B:Chemical, 2022, 373:132706.
[19] MARCELO G A, PIRES S M G, FAUSTINO M A F, et al.New dual colorimetric/fluorimetric probes for Hg2+ detection & extraction based on mesoporous SBA-16 nanoparticles containing porphyrin or rhodamine chromophores[J].Dyes and Pigments, 2019, 161:427-437.
[20] 王莹菲,孟潇,鞠熀先,等.自阵列液滴适配体电化学传感器检测细胞因子干扰素-γ[J].分析化学,2022,50(11):1743-1749.
WANG Y F, MENG X, JU H X, et al.Self-patterned droplet aptasensor for electrochemical detection of human interferon-gamma[J].Chinese Journal of Analytical Chemistry, 2022, 50(11):1743-1749.
[21] 徐群博, 谭红霞, 马良.基于特异性适配体封盖介孔纳米材料的T-2毒素快速检测技术[J].食品科学, 2020, 41(22):324-329.
XU Q B, TAN H X, MA L.A rapid technique for the detection of T-2 toxin based on specific aptamer-capped mesoporous nanomaterials[J].Food Science, 2020, 41(22):324-329.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.