This study aimed to screen the yeast strains with high gas production speed and good freezing resistance and improve the sensory deterioration of raw dough caused by freezing.In this study, five yeast strains were isolated from old dough, and the freezing tolerance of the yeasts was evaluated in terms of growth curve, fermentation power, and survival after freezing.The effects of the isolated yeast and the commercial yeast agent on frozen dough were compared and analyzed by texture characteristics, volatile flavor components, and sensory measurement.Results showed that Y2 of the 5 strains had good frost resistance and gas production, and the dough with Y2 added had better chewability and hardness, which could maintain the texture of the product.Molecular biology identified Y2 as a strain of Saccharomyces cerevisiae, and the differences in sensory and flavor substances between Y2 and three commercially available yeast preparations of dough frozen under the same conditions were compared.The textural and organoleptic results showed that the freeze-tolerant yeast Y2 had an improved effect on the quality of dough after freezing, and was significantly better than the commercially available common yeast in terms of chewiness and organization.On the difference of volatile flavor components, 54 flavor components were detected in group Y2, including 14 alcohols, 13 aldehydes, 14 esters, 7 acids, 1 ketone, and 5 alkanes.Partial least squares discriminant analysis (PLS-DA) was used to analyze the relative contents of 21 kinds of aroma substances in each group of dough.According to the weight coefficient of variables (VIP≥1), 11 major flavor contributing substances were screened.The key flavor components of the Y2 and S3 groups had some similarities, and the key flavor components were more abundant.Using the relative content to plot the heat map, the content of key flavor substances in Y2 was significantly higher than that of the other groups, and the application effect and flavor promotion in the frozen dough were better than that of the common yeast agents.
[1] ZHAO A H, SHI P W, YANG R Q, et al.Isolation of novel wheat bran antifreeze polysaccharides and the cryoprotective effect on frozen dough quality[J].Food Hydrocolloids, 2022, 125:107446.
[2] LUO W H, SUN D W, ZHU Z W, et al.Improving freeze tolerance of yeast and dough properties for enhancing frozen doughquality—A review of effective methods[J].Trends in Food Science & Technology, 2018, 72:25-33.
[3] 李海峰, 吕玉兰, 吴子君, 等.冷冻面团中酵母菌抗冻机制研究进展[J].河南工业大学学报(自然科学版), 2023, 44(4):130-137.
LI H F, LYU Y L, WU Z J, et al.Research progress on antifreeze mechanism of yeast in frozen dough[J].Journal of Henan University of Technology (Natural Science Edition), 2023, 44(4):130-137.
[4] LIU T J, LI Y, SADIQ F A, et al.Predominant yeasts in Chinese traditional sourdough and their influence on aroma formation in Chinese steamed bread[J].Food Chemistry, 2018, 242:404-411.
[5] QIAN X J, GU Y J, SUN B H, et al.Changes of aggregation and structural properties of heat-denatured gluten proteins in fast-frozen steamed bread during frozen storage[J].Food Chemistry, 2021, 365:130492.
[6] 路雪纯, 辛嘉英, 张根生.耐冻藏酵母冷冻生包子生产工艺[J].食品工业, 2021, 42(1):41-46.
LU X C, XIN J Y, ZHANG G S.The production technology of frozen raw steamed bun with freeze resistant storage yeast[J].The Food Industry, 2021, 42(1):41-46.
[7] 沈鑫. 抗冻酵母在酸面团面包加工中的应用[D].北京:北京农学院, 2023.
SHEN X.Application of antifreeze yeast in sour dough bread processing[D].Beijing:Beijing University of Agriculture, 2023.
[8] 郭璐楠. 面团冻藏过程中酵母稳定性变化及其对面团品质的影响[D].无锡:江南大学, 2021.
GUO L N.Variation of yeast stability during dough freezing and its influence on dough quality[D].Wuxi:Jiangnan University, 2021.
[9] XU D, ZHANG Y, TANG K X, et al.Effect of mixed cultures of yeast and lactobacilli on the quality of wheat sourdough bread[J].Frontiers in Microbiology, 2019, 10:2113.
[10] LI H F, DAI F J, ZHANG L L, et al.Characterization of scalded dough and its impact on the growth of mixed yeasts originating from Jiaozi[J].Food Bioscience, 2022, 49:101920.
[11] 李欢欢, 曾雪莹, 谢娟, 等.产香酵母的筛选鉴定及产酯条件优化[J].中国酿造, 2022, 41(6):87-92.
LI H H, ZENG X Y, XIE J, et al.Screening and identification of aroma-producing yeasts and optimization of ester production conditions[J].China Brewing, 2022, 41(6):87-92.
[12] 孟园, 马艳蕊, 姚旖旎, 等.酵母菌的益生功能及其在食品中的应用研究进展[J].中国果菜, 2023, 43(6):28-34.
MENG Y, MA Y R, YAO Y N, et al.Research progress on the beneficial effects and applications of yeasts in foods[J].China Fruit & Vegetable, 2023, 43(6):28-34.
[13] LU L, ZHU K X, YANG Z, et al.Metabolomics analysis of freeze-thaw tolerance enhancement mechanism of ε-poly-l-lysine on industrial yeast[J].Food Chemistry, 2022, 382:132315.
[14] 刘海冬, 刘伟, 刘永乐, 等.鱼胶原肽对面包酵母及冷冻面团的抗冻保护作用[J].食品与发酵工业, 2021, 47(12):70-74.
LIU H D, LIU W, LIU Y L, et al.Cryoprotective effect of fish collagen peptides on baker’s yeast and frozen dough[J].Food and Fermentation Industries, 2021, 47(12):70-74.
[15] LUO W H, SUN D W, ZHU Z W, et al.Improving freeze tolerance of yeast and dough properties for enhancing frozen dough quality—A review of effective methods[J].Trends in Food Science & Technology, 2018, 72:25-33.
[16] DONG J, CHEN D D, WANG G L, et al.Improving freeze-tolerance of baker’s yeast through seamless gene deletion of NTH1 and PUT1[J].Journal of Industrial Microbiology & Biotechnology, 2016, 43(6):817-828.
[17] 杨锦胜, 沙文轩, 吴林珊, 等.不同食用胶体对馒头食用品质及消化特性的影响[J].食品研究与开发, 2024, 45(5):131-138.
YANG J S, SHA W X, WU L S, et al.Effects of different edible colloids on edible quality and digestive characteristics of steamed bread[J].Food Research and Development, 2024, 45(5):131-138.
[18] 苏安祥, 杨琴, 李文, 等.阿魏酸对全麦面团热机械特性及全麦馒头质构品质的改善作用[J].食品科学, 2024, 45(5):24-30.
SU A X, YANG Q, LI W, et al.Effect of ferulic acid on thermo-mechanical properties of whole wheat dough and texture quality of whole wheat steamed bread[J].Food Science, 2024, 45(5):24-30.
[19] LIU Q, GUO X N, ZHU K X.Effects of frozen storage on the quality characteristics of frozen cooked noodles[J].Food Chemistry, 2019, 283:522-529.
[20] YANG S, JEONG S, LEE S Y.Elucidation of rheological properties and baking performance of frozen doughs under different thawing conditions[J].Journal of Food Engineering, 2020, 284:110084.
[21] 席金忠. 馒头制作过程中风味物质的演变与调控[D].无锡:江南大学, 2022.
XI J Z.Evolution and regulation of flavor substances in the process of making steamed bread[D].Wuxi:Jiangnan University, 2022.
[22] 赵畅雯, 潘晓倩, 成晓瑜, 等.产香酵母发酵面团挥发性风味物质分析[J].中国酿造, 2023, 42(1):147-154.
ZHAO C W, PAN X Q, CHENG X Y, et al.Analysis of volatile flavor compounds in dough fermented by aroma-producing yeast[J].China Brewing, 2023, 42(1):147-154.
[23] 高雪, 孙靖, 王俊平.不同品种小麦发酵面团及馒头挥发性物质的差异分析[J].食品安全质量检测学报, 2023, 14(14):10-18.
GAO X, SUN J, WANG J P.Analysis on the differences of volatile substances in different wheat fermented dough and steamed bread[J].Journal of Food Safety & Quality, 2023, 14(14):10-18.
[24] WU C, LIU R S, HUANG W N, et al.Effect of sourdough fermentation on the quality of Chinese Northern-style steamed breads[J].Journal of Cereal Science, 2012, 56(2):127-133.
[25] 彭斌, 胡文婷, 胡祥飞, 等.基于固相微萃取-气相色谱-质谱法分析热风干燥对草鱼肉挥发性风味物质的影响[J].食品安全质量检测学报, 2022, 13(18):5786-5794.
PENG B, HU W T, HU X F, et al.Effects of hot air drying on volatile flavor compounds of Ctenopharyngodon idella meat based on solid-phase microextraction-gas chromatography-mass spectrometry[J].Journal of Food Safety & Quality, 2022, 13(18):5786-5794.
