Food and Fermentation Industries >

2025 , Vol. 51 >Issue 9: 152 - 159

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039673

Preparation and in vitro antioxidant and hypoglycemic activities of water-soluble yeast β-glucan

  • LI Saifen ,
  • CAO Huaqiang ,
  • LI Han ,
  • ZHAO Chenchen ,
  • ZHANG Pengpai
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  • 1(School of Life Sciences, Henan University, Kaifeng 475004, China)
    2(Engineering Research Center for Applied Microbiology of Henan Province, Kaifeng 475004, China)

Received date: 2024-04-24

  Revised date: 2024-06-06

  Online published: 2025-05-28

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Abstract

In this study, an effective protocol for the preparation of water-soluble yeast β-D-glucan (WSYG) was established.By means of dimethyl sulfoxide(DMSO) extraction and ethanol precipitation, WSYG was prepared from brewer's yeast cell wall.The optimal process conditions were determined by response surface analysis.Besides, the physicochemical properties of WSYG were investigated using HPLC, GC, FT-IR, and Congo red test.And its antioxidant and hypoglycemic activities were evaluated through in vitro experiments.The results indicated that the maximum yield of WSYG was (16.38±0.17)% and the polysaccharide content was (96.02±1.37)%, using the DMSO-dissolved ethanol precipitation method with the conditions of 90% (v/v) DMSO concentration, 91% (v/v) ethanol concentration, and a 4.4∶1 volume ratio of ethanol to DMSO solution.The relative molecular mass of the polysaccharide was 602.56 kDa, and monosaccharide composition analysis showed that it consisted solely of D-glucose, and the infrared spectrogram displayed characteristic absorption peaks of polysaccharides with a β-configuration.Furthermore, the atomic force microscopy and the Congo red test demonstrated that it existed in a chain form and did not possess a triple helix structure in aqueous solution, which might contribute to the increased water-solubility of yeast β-glucan.The in vitro activity assays revealed that WSYG had excellent superoxide anion scavenging ability and inhibitory effects on α-amylase and α-glucosidase, exhibiting certain hypoglycemic activity.This approach is facile and practical, and the resulting WSYG demonstrates high purity and good activity.The findings of this study can provide a theoretical basis for the further development and utilization of yeast β-glucan.

Key words： yeast cell wall; water-soluble β-glucan; antioxidant activity; hypoglycemic activity

Cite this article

LI Saifen , CAO Huaqiang , LI Han , ZHAO Chenchen , ZHANG Pengpai . Preparation and in vitro antioxidant and hypoglycemic activities of water-soluble yeast β-glucan[J]. Food and Fermentation Industries, 2025 , 51(9) : 152 -159 . DOI: 10.13995/j.cnki.11-1802/ts.039673

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