Effects and mechanisms of inhibition on Helicobacter pylori by polyphenolic catechin from sea buckthorn in vitro

XIE Kunhao; DAI Shuang; XI Kangbo; WANG Shulin

doi:10.13995/j.cnki.11-1802/ts.041641

Food and Fermentation Industries >

2025 , Vol. 51 >Issue 8: 112 - 118

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041641

Effects and mechanisms of inhibition on Helicobacter pylori by polyphenolic catechin from sea buckthorn in vitro

XIE Kunhao ,
DAI Shuang ,
XI Kangbo ,
WANG Shulin

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(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

Received date: 2024-11-20

Revised date: 2024-12-28

Online published: 2025-05-28

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Abstract

To investigate the effect of inhibitory and mechanisms against Helicobacter pylori by polyphenolic catechin compounds from sea buckthorn berries, the minimum inhibitory concentration was determined by the paper disk and broth microdilution.The antibacterial mechanism of polyphenolic catechins from sea buckthorn against H.pylori was explored by examining their impact on cell membrane permeability, leakage of cellular contents, membrane proteins, and urease activity.Results indicated that epigallocatechin gallate and epigallocatechin exhibited good antibacterial ability, with inhibition zones reaching 17.06 and 22.59 mm.Inhibition rate of two compounds of epigallocatechin gallate and epigallocatechin to H.pylori both could reach more than 50% at the minimal inhibit concentration (MIC).The epigallocatechin gallate showed the best antibacterial ability with an MIC of 156.3 μg/mL.After treating H.pylori by two compounds of epigallocatechin gallate and epigallocatechin with four times concentration of MIC, the cell membrane permeability increased by 0.68 and 0.82 times, the leakage of cellular contents increased by 0.47 and 0.48 times, and the urease activity decreased by 39% and 38% respectively.The above results demonstrated that sea buckthorn polyphenolic compounds, particularly epigallocatechin, and epigallocatechin gallate, having an inhibitory effect on the growth of Helicobacter pylori and exhibitting significant antibacterial ability.

Key words： sea buckthorn polyphenols; Helicobacter pylori; catechins; inhibition activity; mechanism

Cite this article

XIE Kunhao , DAI Shuang , XI Kangbo , WANG Shulin . Effects and mechanisms of inhibition on Helicobacter pylori by polyphenolic catechin from sea buckthorn in vitro[J]. Food and Fermentation Industries, 2025 , 51(8) : 112 -118 . DOI: 10.13995/j.cnki.11-1802/ts.041641

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