Food and Fermentation Industries >

2025 , Vol. 51 >Issue 8: 186 - 194

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039485

Identification and analysis of a high-yield protease salt-tolerant Bacillus altitudinis

  • LI Yulin ,
  • WANG Xia ,
  • WANG Yaming ,
  • LI Qiyue ,
  • SHA Sha ,
  • GUO Zixian ,
  • XING Xiang
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  • (Marine College, Shandong University, Weihai 264209, China)

Received date: 2024-04-08

  Revised date: 2024-05-20

  Online published: 2025-05-28

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Abstract

Marine sediments were sampled from the coastal of Mazigang, Weihai city, Shandong province.This study aimed to screen proteinase-producing bacterial strains and explore their characteristics, growth conditions, and enzyme activities.The strains were identified through morphological observation, physiological and biochemical tests, 16S rDNA sequence analysis, and genomic analysis.The optimal growth conditions of the strain were optimized by using single factor combined with response surface experiment, and the protease activity of the strain was measured under these conditions.The isolated strain was identified as Bacillus altitudinis, with a salinity growth range of 0-160 g/L.The optimal growth conditions for this strain were as follows:temperature 40 ℃, initial pH 9, shaker speed 229 r/min, salinity 30 g/L, optimal carbon source for glucose, and peptone as the preferred nitrogen source.Under these conditions, the protease activity reached 99.747 U.The screened Bacillus altitudinis A1 had good protease production ability, salt tolerance, and heat resistance, which could provide biological basis for food waste degradation, industrial wastewater treatment, microbial fertilizer production, and production process adjustment.

Key words： Bacillus altitudinis; separation and identification; response surface methodology; proteases

Cite this article

LI Yulin , WANG Xia , WANG Yaming , LI Qiyue , SHA Sha , GUO Zixian , XING Xiang . Identification and analysis of a high-yield protease salt-tolerant Bacillus altitudinis[J]. Food and Fermentation Industries, 2025 , 51(8) : 186 -194 . DOI: 10.13995/j.cnki.11-1802/ts.039485

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