Food and Fermentation Industries >

2025 , Vol. 51 >Issue 10: 56 - 62

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038782

Mutation breeding and its liquid fermentation kinetics analysis of bacteriocin high-yielding strains

  • LI Mengyao ,
  • XU Jingyu ,
  • XIAO Sisheng ,
  • YANG Daikang ,
  • XU Jingyu ,
  • YAO Mingyin ,
  • HUANG Lin
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  • 1(College of Biological Science and Engineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang 330045, China)
    2(Jiangxi Tecon Technology Limited Company, Nanchang 330029, China)
    3(College of Engineering, Jiangxi Agricultural University, Jiangxi Key Laboratory of Modern Agricultural Equipment, Nanchang 330045, China)

Received date: 2024-01-31

  Revised date: 2024-06-25

  Online published: 2025-06-11

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Abstract

This study aimed to increase the content of the novel bacteriocin Plantarum C010 produced by Lactobacillus plantarum C010.L.plantarum C010 was mutagenized by ultraviolet radiation, nitrite, acridine orange, and laser, and double plate antagonistic inhibition zone method and high-performance liquid chromatography were used to screen out the high-yielding mutant strains.Results showed that all four mutagenesis treatments could improve the production of bacteriocin Plantarum C010.The mutant strain JG-2 that was mutagenized and screened out by laser reached 17.79 mg/mL on bacteriocin Plantarum C010 production, which was 85.31% higher than the original strain C010 (9.60 mg/mL).Furthermore, the kinetic analysis of liquid batch fermentation showed that the model fitting determination coefficients (R2) of cell growth, total sugar consumption, and product synthesis of strains C010 and JG-2 were better than others.Bacteriocin synthesis of the two strains was partially cell growth-coupled, and the bacteriocin synthesis parameters of the mutant strain JG-2 were better than other original strain C010.The results provide data basis and research ideas to improve bacteriocin Plantarum C010 production.

Key words： Lactobacillus plantarum C010; bacteriocin; mutation breeding; high-yielding strain; kinetics analysis

Cite this article

LI Mengyao , XU Jingyu , XIAO Sisheng , YANG Daikang , XU Jingyu , YAO Mingyin , HUANG Lin . Mutation breeding and its liquid fermentation kinetics analysis of bacteriocin high-yielding strains[J]. Food and Fermentation Industries, 2025 , 51(10) : 56 -62 . DOI: 10.13995/j.cnki.11-1802/ts.038782

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