Difference of enzyme metabolites in Xinjiang seabuckthorn during different fermentation periods based on non-targeted metabolomics

  • FAN Rui ,
  • WANG Wenwen ,
  • WANG Tengbin ,
  • LU Bin ,
  • LIU Xiao ,
  • CAO Xueqin
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  • (Xinjiang Academy of Analysis and Testing, Urumqi 830011, China)

Received date: 2024-04-17

  Revised date: 2024-06-07

  Online published: 2025-06-11

Abstract

In this study, LC-MS non-targeted metabolomics technology was utilized to analyze diverse metabolic compounds produced by seabuckthorn enzymes across different fermentation periods.Methods including partial least squares discriminant analysis (PLS-DA), principal component analysis (PCA), HMDB compound classification, KEGG pathway enrichment, Venn mapping, and volcano plotting were employed to evaluate the metabolome data.A total of 1 164 metabolites were identified and classified into 16 distinct classes.The majority were organic acids, organic heterocyclic compounds, and lipids, prominently involved in the citric acid cycle (TCA cycle), butyric acid metabolism, and tryptophan metabolism.Using the criteria of variable importance in projection value ≥ 1, P-value<0.05, FC>2, and FC<1/2 in the PLS-DA model, 7 metabolites with significant differences were identified.This study aimed to elucidate the changes in metabolic substance content during various fermentation periods and explore active substance components, providing a scientific foundation for the functional components and metabolic regulation mechanisms of seabuckthorn enzymes.This research lays the theoretical groundwork for the future development and utilization of seabuckthorn resources, facilitating the advancement of the seabuckthorn industry.

Cite this article

FAN Rui , WANG Wenwen , WANG Tengbin , LU Bin , LIU Xiao , CAO Xueqin . Difference of enzyme metabolites in Xinjiang seabuckthorn during different fermentation periods based on non-targeted metabolomics[J]. Food and Fermentation Industries, 2025 , 51(10) : 167 -176 . DOI: 10.13995/j.cnki.11-1802/ts.039604

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