Advances in solubilization modification technology and its application of yeast β-glucan

  • ZOU Yan ,
  • YUAN Peng ,
  • ZHOU Fang ,
  • YAN Mengqing ,
  • LI Haizhi ,
  • ZENG Xueying ,
  • WANG Haotian ,
  • ZHANG Tianyu ,
  • LIU Guoyu ,
  • DUAN Shenglin
Expand
  • (China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, Beijing 100015, China)

Received date: 2024-07-02

  Revised date: 2024-08-21

  Online published: 2025-06-11

Abstract

Yeast β-glucan is a functional polysaccharide that exhibits abundant bioactivity within the yeast cell wall.However, the dense triple-helical structure of yeast β-glucan leads to poor solubility, which greatly restricts the application potential of yeast β-glucan.Therefore, the strategies for modifying yeast β-glucan to enhance its solubility have been a popular research topic in recent years.This article comprehensively reviews the source and structure of yeast β-glucan and explores the advancements in solubility-enhancing modification methods both domestically and internationally.Furthermore, it outlines the current application status of yeast β-glucan in the food, pharmaceutical, and cosmetic industries.With a primary emphasis on the physical, chemical, and biological modification approaches for yeast β-glucan, the paper analyzes the characteristics of various solubility enhancement techniques and discusses their future development trends.

Cite this article

ZOU Yan , YUAN Peng , ZHOU Fang , YAN Mengqing , LI Haizhi , ZENG Xueying , WANG Haotian , ZHANG Tianyu , LIU Guoyu , DUAN Shenglin . Advances in solubilization modification technology and its application of yeast β-glucan[J]. Food and Fermentation Industries, 2025 , 51(10) : 376 -385 . DOI: 10.13995/j.cnki.11-1802/ts.040364

References

[1] YUAN H J, LAN P, HE Y, et al.Effect of the modifications on the physicochemical and biological properties of β-glucan-a critical review[J].Molecules, 2019, 25(1):57.
[2] ROSS P, FARRELL M P.The road to structurally defined β-glucans[J].Chemical Record, 2021, 21(11):3178-3193.
[3] WOUK J, DEKKER R F H, QUEIROZ E A I F, et al.β-Glucans as a Panacea for a healthy heart? Their roles in preventing and treating cardiovascular diseases[J].International Journal of Biological Macromolecules, 2021, 177:176-203.
[4] BAI J Y, REN Y K, LI Y, et al.Physiological functionalities and mechanisms of β-glucans[J].Trends in Food Science & Technology, 2019, 88:57-66.
[5] CARUSO M A, PIERMARIA J A, ABRAHAM A G, et al.β-glucans obtained from beer spent yeasts as functional food grade additive:Focus on biological activity[J].Food Hydrocolloids, 2022, 133:107963.
[6] ZHENG Z M, HUANG Q L.New insight into the structure-dependent two-way immunomodulatory effects of water-soluble yeast β-glucan in macrophages[J].Carbohydrate Polymers, 2022, 291:119569.
[7] ZHENG Z M, HUANG Q L, KANG Y, et al.Different molecular sizes and chain conformations of water-soluble yeast β-glucan fractions and their interactions with receptor Dectin-1[J].Carbohydrate Polymers, 2021, 273:118568.
[8] XIN Y J, JI H, CHO E, et al.Immune-enhancing effect of water-soluble beta-glucan derived from enzymatic hydrolysis of yeast glucan[J].Biochemistry and Biophysics Reports, 2022, 30:101256.
[9] LIU J J, HOU Y K, WANG X, et al.Recent advances in the biosynthesis of fungal glucan structural diversity[J].Carbohydrate Polymers, 2024, 329:121782.
[10] MAGNELLI P, CIPOLLO J F, ABEIJON C.A refined method for the determination of Saccharomyces cerevisiae cell wall composition and β-1, 6-glucan fine structure[J].Analytical Biochemistry, 2002, 301(1):136-150.
[11] HORNECK JOHNSTON C J H, LEDWITH A E, LUNDAHL M L E, et al.Recognition of yeast β-glucan particles triggers immunometabolic signaling required for trained immunity[J].iScience, 2024, 27(3):109030.
[12] 骆莹. 酵母细胞去除猕猴桃果汁中展青霉素的机理研究及磁性吸附剂的制备[D].杨凌:西北农林科技大学, 2016.
LUO Y.Patulin absorption mechanism study on yeast cells from kiwi fruit juice and magnetic adsorbents preparation[D].Yangling:Northwest A & F University, 2016.
[13] 韩瑨, 吴正钧, 高彩霞, 等.功能性多糖饮料的研究进展[J].食品研究与开发, 2015, 36(8):115-119.
HAN J, WU Z J, GAO C X, et al.Progress in the research and development of functional polysaccharide beverages[J].Food Research and Development, 2015, 36(8):115-119.
[14] WANG D M, KIM D H, YOON J J, et al.Production of high-value β-1, 3-glucooligosaccharides by microwave-assisted hydrothermal hydrolysis of curdlan[J].Process Biochemistry, 2017, 52:233-237.
[15] HUANG X Y, LI C Y, XI J.Dynamic high pressure microfluidization-assisted extraction of plant active ingredients:A novel approach[J].Critical reviews in food science and nutrition, 2022, 63(33):12413-12421.
[16] WANG Y J, XIONG X, HUANG G L.Ultrasound-assisted extraction and analysis of maidenhairtree polysaccharides[J].Ultrasonics Sonochemistry, 2023, 95:106395.
[17] LEE H N, LIM H J, PARK J Y, et al.Effect of modification methods on the physical properties and immunomodulatory activity of particulate β-glucan[J].Food Science and Biotechnology, 2024, 33(7):1615-1621.
[18] ZHOU Z B, XIAO J W, HUANG S, et al.A wet-adhesive carboxymethylated yeast β-glucan sponge with radical scavenging, bacteriostasis and anti-inflammatory functions for rapid hemostasis[J].International Journal of Biological Macromolecules, 2023, 230:123158.
[19] ZHANG Q Y, XIE J, XUE B, et al.Effect of sulfated modification on rheological and physiological properties of oat β-glucan oligosaccharides prepared by acid or oxidative degradation[J].Journal of Cereal Science, 2021, 99:103209.
[20] 郭欢. 青稞β-葡聚糖的提取分离、结构表征、化学修饰及其生物活性研究[D].雅安:四川农业大学, 2020.
GUO H.Extraction, isolation, structural characterization chemical modification, and biological activities of Qingke (Hordeum vulgare L.) β-glucans[D].Ya′an:Sichuan Agricultural University, 2020.
[21] 王亚静. 酵母β-葡聚糖改性及其定量检测方法研究[D].北京:北京化工大学, 2020.
WANG Y J.Study on modification of yeast β-glucan and its quantitative detection method[D].Beijing:Beijing University of Chemical Technology, 2020.
[22] ISHIMOTO Y, ISHIBASHI K I, YAMANAKA D, et al.Modulation of an innate immune response by soluble yeast β-glucan prepared by a heat degradation method[J].International Journal of Biological Macromolecules, 2017, 104:367-376.
[23] 陈亮, 武小芬, 齐慧, 等.电子束辐照对不同含水量芦苇木质纤维素结构及酶解性能的影响[J].辐射研究与辐射工艺学报, 2023, 41(2):76-85.
CHEN L, WU X F, QI H, et al.Effects of electron beam irradiation pretreatment on the structure and enzymatic efficiency of Phragmites australis lignocelluloses with different moisture contents[J].Journal of Radiation Research and Radiation Processing, 2023, 41(2):76-85.
[24] 刘媛媛. 酵母细胞壁多糖制备及流变学性质研究[D].北京:中国农业科学院, 2010.
LIU Y Y.Study on preparation of cell wall polysaccharide from Saccharomyces cerevisiae and its rheological properties[D].Beijing:Chinese Academy of Agricultural Sciences, 2010.
[25] LONG N T, ANH N T N, GIANG B L, et al.Radiation degradation of β-glucan with a potential for reduction of lipids and glucose in the blood of mice[J].Polymers, 2019, 11(6):955.
[26] 乜世成. 辐照降解裸藻β-葡聚糖及其产物的抗氧化活性研究[D].西宁:青海师范大学, 2022.
MEI S C.Degradation of euglena β-glucan by electron beam irradiation and antioxidant activity[D].Xining:Qinghai Normal University, 2022.
[27] LI H T, ZHANG W Y, CHEN Y Y, et al.Physical modification of high amylose starch using electron beam irradiation and heat moisture treatment:The effect on multi-scale structure and in vitro digestibility[J].Food Chemistry, 2023, 424:136344.
[28] LIU H Z, BAI W Q, HE L, et al.Degradation mechanism of Saccharomyces cerevisiae β-D-glucan by ionic liquid and dynamic high pressure microfluidization[J].Carbohydrate Polymers, 2020, 241:116123.
[29] 白文强. 基于离子液体与微射流协同作用的酵母β-葡聚糖增溶机制研究[D].北京:中国农业科学院, 2019.
BAI W Q.Dissolution mechanisms of yeast β-glucan modified by the ionic liquid combined with high pressure microfluidization[D].Beijing:Chinese Academy of Agricultural Sciences, 2019.
[30] HUANG C, MIAO M, JIANG B, et al.Polysaccharides modification through green technology:Role of ultrasonication towards improving physicochemical properties of (1-3)(1-6)-α-D-glucans[J].Food Hydrocolloids, 2015, 50:166-173.
[31] CHEUNG Y C, YIN J Y, WU J Y.Effect of polysaccharide chain conformation on ultrasonic degradation of curdlan in alkaline solution[J].Carbohydrate Polymers, 2018, 195:298-302.
[32] HUANG S Y, CHEN F, CHENG H, et al.Modification and application of polysaccharide from traditional Chinese medicine such as Dendrobium officinale[J].International Journal of Biological Macromolecules, 2020, 157:385-393.
[33] MEILING C, TIANCHEN J, JIANQUAN H, et al.Functionalization of sodium carboxymethylated yeast β-glucan by epigallocatechin gallate:Antioxidant activity and color stability[J].Journal of the Chinese Chemical Society, 2021,68(8):1413-1422.
[34] ZHANG H, ZHANG J, FAN Z L, et al.Chemical synthesis of sulfated yeast (Saccharomyces cerevisiae) glucans and their in vivo antioxidant activity[J].Molecules, 2017, 22(8):1266.
[35] DU L P, ZHANG X K, WANG C, et al.Preparation of water soluble yeast glucan by four kinds of solubilizing processes[J].Engineering, 2012, 4(10):184-188.
[36] CARVALHO V S D, LAURA G D, áNGELES C M, et al.Analysis and application of a suite of recombinant endo-β (1,3)-D-glucanases for studying fungal cell walls[J].Microbial Cell Factories, 2021, 20(1):126.
[37] ZHEN Q, DONG Y, XIN Y, et al.The recognition mechanism of triple-helical β-1,3-glucan by a β-1,3-glucanase[J].Chemical Communications (Cambridge, England), 2017,53(67):9368-9371.
[38] KATSUKI H, NOBUHIRO K, SACHIO H.Characterization and enzymatic hydrolysis of hydrothermally treated β-1,3-1,6-glucan from Aureobasidium pullulans[J].World journal of Microbiology & Biotechnology, 2016,32(12):206.
[39] 段会轲, 熊善柏, 刘海梅.酵母β-1,3-葡聚糖的酶法增溶及产物分析[J].食品科学, 2008, 29(1):185-189.
DUAN H K, XIONG S B, LIU H M.Study on solubility and properties enzymolysates of yeast β-1, 3-glucan[J].Food Science, 2008, 29(1):185-189.
[40] 范红梅. 酵母β-葡聚糖酶解改性及其应用[D].天津:天津科技大学, 2018.
FAN H M.Enzyme modification of yeast β-glucan and its application[D].Tianjin:Tianjin University of Science and Technology, 2018.
[41] 张学况, 杜丽平, 王超, 等.酶解法增溶酵母(1→3)-β-D-葡聚糖的研究[J].食品与发酵工业, 2012, 38(7):48-52.
ZHANG X K, DU L P, WANG C, et al.An enzymolysis method for the solubilization of yeast(1→3)-β-D-glucan[J].Food and Fermentation Industries, 2012, 38(7):48-52.
[42] 杜昱光, 白雪芳, 曲天明, 等.利用蜗牛酶降解天然产物及多糖的方法:中国, CN01136891.8[P].2003-05-14.
DU Y G, BAI X F, QU T M, et al.Method of using snail enzyme to degrade natural products and polysaccharides:China, CN01136891.8[P].2003-05-14.
[43] 张漫莉, 王强, 刘丽, 等.酵母β-葡聚糖增溶改性与构效关系[J].中国食品学报, 2022, 22(5):147-156.
ZHANG M L, WANG Q, LIU L, et al.The solubilization modification and structure-activity relationship of yeast β-glucan[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(5):147-156.
[44] 王国军.一种水溶酵母β-葡聚糖及其制备方法:中国, CN202210982808.9[P].2022-12-16.
WANG G J.A water-soluble yeast β-glucan and its preparation method:China, CN202210982808.9[P].2022-12-16.
[45] YUAN H J, HE Y, ZHANG H, et al.Ultrasound-assisted enzymatic hydrolysis of yeast β-glucan catalyzed by β-glucanase:Chemical and microstructural analysis[J].Ultrasonics Sonochemistry, 2022, 86:106012.
[46] HU H G, ZHAO Q L, XIE J H, et al.Polysaccharides from pineapple pomace:New insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities[J].International Journal of Biological Macromolecules, 2019, 121:1213-1226.
[47] WANG Q, WANG Y, HUANG M G, et al.Ultrasound-assisted alkaline proteinase extraction enhances the yield of pecan protein and modifies its functional properties[J].Ultrasonics Sonochemistry, 2021, 80:105789.
[48] YAN S Z, WANG Q, YU J Y, et al.Ultrasound-assisted preparation of protein-polyphenol conjugates and their structural and functional characteristics[J].Ultrasonics Sonochemistry, 2023, 100:106645.
[49] 袁洪洁. 产朊假丝酵母β-葡聚糖的增溶改性及其活性评价研究[D].上海:上海应用技术大学, 2021.
YUAN H J.Study on solubilization of Candida utilis β-glucan and evaluation of of its activity [D].Shanghai:Shanghai Institute of Technology, 2021.
[50] 郑赵敏. 酵母葡聚糖改性增溶及链结构与免疫调节活性间构效关系的研究[D].武汉:华中农业大学, 2019.
ZHENG Z M.Modification from yeast glucan solubility and the structure-function relationship between chain structure and immunomodulatory activity[D].Wuhan:Huazhong Agricultural University, 2019.
[51] XIU W Y, WANG X, NA Z G, et al.Ultrasound-assisted hydrogen peroxide-ascorbic acid method to degrade sweet corncob polysaccharides can help treat type 2 diabetes via multiple pathways in vivo[J].Ultrasonics Sonochemistry, 2023, 101:106683.
[52] 董琳. 超声波辅助过氧化氢法增溶酵母β-葡聚糖及其对马铃薯淀粉特性影响的研究[D].上海:上海应用技术大学, 2023.
DONG L.Solubiliztion of yeast β-glucan by ultrasonic-assisted H2O2 and its effect on potato starch properties[D].Shanghai:Shanghai Institute of Technology, 2023.
[53] ZHENG Z M, HUANG Q L, LUO X G, et al.Effects and mechanisms of ultrasound- and alkali-assisted enzymolysis on production of water-soluble yeast β-glucan[J].Bioresource Technology, 2019, 273:394-403.
[54] HORAGUCHI Y, TAKAHASHI M, TAKAMATSU K, et al.Heterologous expression of α-1, 3-glucanase Agn1p from Schizosaccharomyces pombe, and efficient production of nigero-oligosaccharides by enzymatic hydrolysis from solubilized α-1, 3;1, 6-glucan[J].Bioscience, Biotechnology, and Biochemistry, 2023, 87(10):1219-1228.
[55] VUSCAN P, KISCHKEL B, HATZIOANNOU A, et al.Potent induction of trained immunity by Saccharomyces cerevisiae β-glucans[J].Frontiers in Immunology, 2024, 15:1323333.
[56] HAN B, BARUAH K, COX E, et al.Structure-functional activity relationship of β-glucans from the perspective of immunomodulation:A mini-review[J].Frontiers in Immunology, 2020, 11:658.
[57] YOON T J, KIM T J, LEE H, et al.Anti-tumor metastatic activity of β-glucan purified from mutated Saccharomyces cerevisiae[J].International Immunopharmacology, 2008, 8(1):36-42.
[58] UPADHYAY T K, TRIVEDI R, KHAN F, et al.In vitro elucidation of antioxidant, antiproliferative, and apoptotic potential of yeast-derived β-1, 3-glucan particles against cervical cancer cells[J].Frontiers in Oncology, 2022, 12:942075.
[59] NO H, KIM J, SEO C R, et al.Anti-inflammatory effects of β-1, 3-1, 6-glucan derived from black yeast Aureobasidium pullulans in RAW264.7 cells[J].International Journal of Biological Macromolecules, 2021, 193:592-600.
[60] QIAO Y, YE X F, ZHONG L L, et al.Yeast β-1, 3-glucan production by an outer membrane β-1, 6-glucanase:Process optimization, structural characterization and immunomodulatory activity[J].Food & Function, 2022, 13(7):3917-3930.
[61] XU M D, MO X X, HUANG H, et al.Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice[J].International Journal of Biological Macromolecules, 2020, 161:258-270.
[62] GOLISCH B, LEI Z H, TAMURA K, et al.Configured for the human gut microbiota:Molecular mechanisms of dietary β-glucan utilization[J].ACS Chemical Biology, 2021, 16(11):2087-2102.
[63] WANG H L, CHEN G J, LI X, et al.Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin[J].Food & Function, 2020, 11(12):10386-10396.
[64] MO X X, SUN Y H, LIANG X L, et al.Insoluble yeast β-glucan attenuates high-fat diet-induced obesity by regulating gut microbiota and its metabolites[J].Carbohydrate Polymers, 2022, 281:119046.
[65] VIOLA C, CHRISTOS E, GIORGOS M, et al.Biotechnological addition of β-glucans from cereals, mushrooms and yeasts in foods and animal feed[J].Processes, 2021,9(11):1889.
[66] ZBIKOWSKA A, KOWALSKA M, ZBIKOWSKA K, et al.Study on the incorporation of oat and yeast β-glucan into shortbread biscuits as a basis for designing healthier and high quality food products[J].Molecules, 2022, 27(4):1393.
[67] BACHA U, NASIR M, IQBAL S, et al.Influence of yeast β-glucan on cookies sensory characteristics and bioactivities[J].Journal of Chemistry, 2018, 2018:1295184.
[68] AL-SAHLANY S T G, AL-KAABI W J, AL-MANHEL A J A, et al.Effects of β-glucan extracted from Saccharomyces cerevisiae on the quality of bio-yoghurts:in vitro and in vivo evaluation[J].Journal of Food Measurement and Characterization, 2022, 16(5):3607-3617.
[69] RAIKOS V, GRANT S B, HAYES H, et al.Use of β-glucan from spent brewer’s yeast as a thickener in skimmed yogurt:Physicochemical, textural, and structural properties related to sensory perception[J].Journal of Dairy Science, 2018, 101(7):5821-5831.
[70] APOSTU P M, MIHOCIU T E, NICOLAU A I.Technological and sensorial role of yeast β-glucan in meat batter reformulations[J].Journal of Food Science and Technology, 2017, 54(9):2653-2660.
[71] HERGESELL K, VALENTOVÁ K, VELEBNY V, et al.Common cosmetic compounds can reduce air pollution-induced oxidative stress and pro-inflammatory response in the skin[J].Skin Pharmacology and Physiology, 2022, 35(3):156-165.
[72] SOUSA P, TAVARES-VALENTE D, AMORIM M, et al.β-Glucan extracts as high-value multifunctional ingredients for skin health:A review[J].Carbohydrate Polymers, 2023, 322:121329.
[73] ZHU J, WANG F Y, SONG S S, et al.Alleviating skin barrier disruption, skin inflammation and pruritus:a moisturizing spray containing β-glucan and panthenol[J].International Journal of Dermatology and Venereology, 2022, 6(1):1-8.
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