Analysis of free amino acid content and volatile oil aroma components of Amomum tsaoko fruits and seeds before and after drying

  • WU Lianzhang ,
  • ZHANG Yingping ,
  • LIAO Fangping ,
  • ZHOU Jilan ,
  • HUANG Mei ,
  • YANG Yi ,
  • MU Mingxing ,
  • HE Yuqiu ,
  • HE Juncai
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  • (Nujiang Green Spice Industry Research Institute, Lushui 673299, China)

Received date: 2024-05-19

  Revised date: 2024-07-24

  Online published: 2025-06-11

Abstract

This study aimed to explore and analyze the differences in physical and chemical quality and volatile oil aroma components of Amomum tsaoko fruit and kernel before and after drying.In this study, fresh Amomum tsaoko fruits and kernels were used as raw materials and smokeless green drying technology was used to dry Amomum tsaoko.The moisture, color difference, volatile oil content, and free amino acid content of Amomum tsaoko fruits and kernels before and after drying were analyzed and detected, and the GC-MS method was used to analyze and identify the aroma components of volatile oil.Results showed that the moisture content of Amomum tsaoko was significantly reduced after drying.The volatile oil content of Amomum tsaoko was higher under the same condition.The L* values of the whole fruit and kernel of Amomum tsaoko were significantly increased, and the a* and b* values of the whole fruit were also significantly increased.Sixteen free amino acids were detected in all four Amomum tsaoko samples, and the free amino acid components were similar.The total amino acid content was 2.09-6.05 g/100 g, and the flavor amino acid content was significantly different.A total of 27 aroma components were detected in four kinds of Amomum tsaoko samples, including 5 terpenes, 9 alcohols, 9 aldehydes, and 4 esters, mainly alcohols and aldehydes.There were more aroma components detected in the volatile oil of dried fruit.The highest relative content of aroma components in fresh fruit and fresh fruit kernel was nerol, and the highest relative content of aroma components in dried fruit and dried fruit kernel was 1,8-cineole.These results demonstrated that there were some differences in moisture, color, and volatile oil content of Amomum tsaoko fruits and kernels before and after drying.The free amino acid content and volatile oil aroma components had both commonalities and certain differences.The results of this study can provide a theoretical reference for the development and utilization of fresh and dried Amomum tsaoko fruits and kernels.

Cite this article

WU Lianzhang , ZHANG Yingping , LIAO Fangping , ZHOU Jilan , HUANG Mei , YANG Yi , MU Mingxing , HE Yuqiu , HE Juncai . Analysis of free amino acid content and volatile oil aroma components of Amomum tsaoko fruits and seeds before and after drying[J]. Food and Fermentation Industries, 2025 , 51(10) : 328 -335 . DOI: 10.13995/j.cnki.11-1802/ts.039926

References

[1] 杨庆宽, 周铁生, 孔繁浩, 等.鲜、干草果精油化学成分及香气味的研究[J].香料香精化妆品, 1994(1):1-5.
YANG Q K, ZHOU T S, KONG F H, et al.Study on chemical composition and aroma of essential oil from fresh and dried fruits[J].Flavour Fragrance Cosmetics, 1994(1):1-5.
[2] 韩林, 汪开拓, 王兆丹, 等.草果精油的化学组成、抗氧化及抑菌活性研究[J].食品工业科技, 2013, 34(14):152-155.
HAN L, WANG K T, WANG Z D, et al.Chemical composition, antioxidant and antibacterial activities of the essential oil from Amomum tsaoko[J].Science and Technology of Food Industry, 2013, 34(14):152-155.
[3] 练强, 杨毅, 吴莲张.怒江草果产业发展存在的问题及对策[J].现代农业科技, 2020(20):234-235.
LIAN Q, YANG Y, WU L Z.Problems and countermeasures of Nujiang Amomum tsao-ko industry development[J].Modern Agricultural Science and Technology, 2020(20):234-235.
[4] 梁淼, 张祉敏, 吴雅健, 等.辛香料草果的研究及精油应用进展[J].食品工业科技, 2023, 44(2):427-435.
LIANG M, ZHANG Z M, WU Y J, et al.Progress on research of spice Amomum tsaoko and application of its essential oil[J].Science and Technology of Food Industry, 2023, 44(2):427-435.
[5] HE X F, ZHANG X K, GENG C G, et al.Tsaokopyranols A-M, 2, 6-epoxydiarylheptanoids from Amomum tsaoko and their α-glucosidase inhibitory activity[J].Bioorganic Chemistry, 2020, 96:103638.
[6] 张雪研, 舒娟, 范江平, 等.草果挥发油的化学成分、生物活性及应用研究进展[J].食品与发酵科技, 2023, 59(5):81-86.
ZHANG X Y, SHU J, FAN J P, et al.Chemical composition, biological activities and application progress of volatile oil from Amomum tsaoko[J].Food and Fermentation Science & Technology, 2023, 59(5):81-86.
[7] 和俊才, 林毅, 吴桂苹, 等.草果酱制备工艺的响应面法优化及挥发性风味成分分析[J].热带农业科学, 2022, 42(6):88-96.
HE J C, LIN Y, WU G P, et al.Optimization of preparation technology by response surface methodology and analysis of volatile flavor components of Amomum tsaoko sauce[J].Chinese Journal of Tropical Agriculture, 2022, 42(6):88-96.
[8] 薛亚杰, 胡荣锁, 姚美芹, 等.草果理化特性分析及其速溶茶饮调配[J].食品工业, 2023, 44(4):5-10.
XUE Y J, HU R S, YAO M Q, et al.Physicochemical properties and instant tea preparation of Amomum tsaoko[J].The Food Industry, 2023, 44(4):5-10.
[9] CHEN G, LI J, SUN Z W, et al.Rapid and sensitive ultrasonic-assisted derivatisation microextraction (UDME) technique for bitter taste-free amino acids (FAA) study by HPLC-FLD[J].Food Chemistry, 2014, 143:97-105.
[10] 吕春茂, 刘畅, 孟宪军, 等.苹果渣发酵过程中游离氨基酸和挥发性香气成分分析[J].食品科学, 2014, 35(18):146-150.
LYU C M, LIU C, MENG X J, et al.Analysis of free amino acids and aroma components in fermented apple pomace[J].Food Science, 2014, 35(18):146-150.
[11] 谷风林, 张林辉, 房一明, 等.云南不同地区草果物理性状、精油含量及组成分析[J].热带作物学报, 2018, 39(7):1440-1446.
GU F L, ZHANG L H, FANG Y M, et al.Analysis of physical properties, essential oil content and composition of Amomum tsao-ko from different origins of Yunnan[J].Chinese Journal of Tropical Crops, 2018, 39(7):1440-1446.
[12] PRAMONO E K, TAUFAN A, NOVRINALDI, et al.Performance tests of loadcell as real-time moisture content sensor:Case study Moringa oleifera leaves drying[J].IOP Conference Series, : Earth and Environmental Science, 2022, 1024(1):012018.
[13] 吴雅璐. 不同干燥方法对花椒叶色泽、挥发性物质及抗氧化性的影响[D].临汾:山西师范大学, 2019.
WU Y L.The effects of different drying methods on the color, volatile matter and antioxidant activity of Zanthoxylum bungeanum leaves[D].Linfen:Shanxi Normal University, 2019.
[14] 井然, 冯雷, 陈丽梅.茶叶中游离氨基酸分析方法的研究进展[J].安徽农业科学, 2010, 38(17):9186-9187;9210.
JING R, FENG L, CHEN L M.Research progress in methods for analyzing free amino acid content in tea[J].Journal of Anhui Agricultural Sciences, 2010, 38(17):9186-9187;9210.
[15] 普岳红, 杨永红, 吴德喜, 等.云南盈江县不同产地草果氨基酸和矿质营养元素分析[J].亚热带植物科学, 2015, 44(4):293-296.
PU Y H, YANG Y H, WU D X, et al.Determination of amino acid and mineral elements in Amomum tsaoko from different provenances in Yingjiang, Yunnan[J].Subtropical Plant Science, 2015, 44(4):293-296.
[16] 陈玉芹, 史文斌, 禇勇, 等.马关县不同产地草果氨基酸组成与分析[J].食品安全质量检测学报, 2022, 13(18):6090-6096.
CHEN Y Q, SHI W B, ZHE Y, et al.Amino acid composition and analysis of Amomum tsaoko from different producing areas in Maguan County[J].Journal of Food Safety & Quality, 2022, 13(18):6090-6096.
[17] 杨洪锦, 吴长喜, 田应菊, 等.新鲜草果营养成分及风味物质分析[J].食品工业科技, 2024,45(20):271-278.
YANG H J,WU C X,TIAN Y J, et al.Analysis of nutrient composition and flavor substances of fresh Amomum tsaoko[J].Science and Technology of Food Industry, 2024,45(20):271-278.
[18] PELLETT P L, YONG V R.Nutritional evaluation of protein foods[M].Tokyo:The United National University Publishing Company, 1980.
[19] 孙灵霞. 八角茴香对卤鸡肉挥发性风味的影响及其作用机制[D].西安:陕西师范大学, 2014.
SUN L X.The effect of star anise on the volatile flavor of stewed chicken and its mechanism[D].Xi′an:Shaanxi Normal University, 2014.
[20] 许倬卉, 杨绍兵, 杨天梅, 等.不同干燥程度草果果实形态及种仁挥发油的变化规律[J].中国调味品, 2021, 46(10):21-26.
XU Z H, YANG S B, YANG T M, et al.The changes of fruit morphology of Amomum tsaoko with different drying degrees and volatile oil of seed kernel[J].China Condiment, 2021, 46(10):21-26.
[21] 胡智慧, 白佳伟, 杨文熙, 等.新鲜草果中关键香气成分的分析[J].食品科学, 2020, 41(16):173-178.
HU Z H, BAI J W, YANG W X, et al.Identification of the key odorants in fresh Amomum tsaoko fruit[J].Food Science, 2020, 41(16):173-178.
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