Food and Fermentation Industries >

2025 , Vol. 51 >Issue 11: 65 - 74

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039540

Inhibitory effect of apple polyphenols on Pseudomonas aeruginosa based on network pharmacology and molecular docking

  • GUO Shiji ,
  • HE Chenghong ,
  • LIU Zheng ,
  • GUO Kexin ,
  • WANG Hui
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  • 1(College of Food and Health, Jinzhou Medical University, Jinzhou 121001, China)
    2(Department of Paediatrics, Enliang Hospital of Taian County, Anshan 114100, China)

Received date: 2024-04-12

  Revised date: 2024-07-05

  Online published: 2025-06-19

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Abstract

This study used network pharmacology and molecular docking technology to explore the inhibitory mechanism of apple polyphenols on Pseudomonas aeruginosa and its diseases.The active components of apple polyphenols were collected through references search, and the component targets were obtained through TCMSP platform, PubChem database, SwissTargetPrediction database, and Swiss ADME website.Genecards, OMIM, and TTD databases were searched for Pseudomonas aeruginosa related targets.The “bioinformatics” website was used to obtain intersection targets.The protein-protein interaction analysis of the intersection targets was performed using the STRING platform.Moreover, the GO functional enrichment analysis and KEGG pathway enrichment analysis were performed using the DAVID platform.Finally, molecular docking was performed using the AutoDockTools software to validate the experiment.After screening, 8 plant active components and 184 component targets were obtained, including 1 103 targets for inhibiting Pseudomonas aeruginosa and 52 intersection targets.Through protein-protein interaction analysis, a total of 51 key targets including EGFR, AKT1, BCL2, and HIF1A were predicted.Through GO and KEGG enrichment analysis, 387 entries and 93 pathways were found to be related to the inhibition of Pseudomonas aeruginosa, mainly affecting pathways such as EGFR tyrosine kinase inhibitor resistance, PI3K/AKT, and VEGF signaling pathways.Molecular docking showed that the four active components with the highest degree values could all bind to the targets.Overall experimental results showed that apple polyphenols can inhibit Pseudomonas aeruginosa and its diseases through multiple components, multiple targets, and multiple pathways.This study can provide a theoretical basis for the study of apple polyphenols in inhibiting Pseudomonas aeruginosa and its diseases.

Key words： apple polyphenols; Pseudomonas aeruginosa; network pharmacology; molecular docking; antibacterial activity

Cite this article

GUO Shiji , HE Chenghong , LIU Zheng , GUO Kexin , WANG Hui . Inhibitory effect of apple polyphenols on Pseudomonas aeruginosa based on network pharmacology and molecular docking[J]. Food and Fermentation Industries, 2025 , 51(11) : 65 -74 . DOI: 10.13995/j.cnki.11-1802/ts.039540

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