This study presents a low-cost and environmentally friendly method for the green synthesis of silver nanoparticles (AgNPs) using Forsythia suspensa leaf extract as a reducing agent.The optimal green synthesis process was determined through single-factor and orthogonal experiments;The structural characterization of AgNPs was conducted using UV-Vis spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction;The catalytic degradation activity of AgNPs on organic pollutants such as p-nitrophenol (4-NP), methyl orange, methylene blue, and rhodamine B was evaluated;The antibacterial activity against Escherichia coli (E.coli) was preliminarily assessed;The antioxidant activity was evaluated by determining the DPPH radical scavenging ability, with vitamin C used as a positive control.The results showed that the optimal conditions for green synthesis were 40% ethanol concentration, 70 minutes reaction time, and 4 mL of Forsythia suspensa leaf extract. Under these conditions, the synthesized AgNPs had particle sizes mainly ranging from 40 to 70 nm with good crystallinity.The degradation rates of 4-NP, methylene blue, methyl orange, and rhodamine B by AgNPs were 91.08%, 90.03%, 94.64%, and 93.00%, respectively.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against E.coli were determined to be 0.140 6 mg/mL and 0.281 3 mg/mL, respectively;The DPPH radical scavenging rate was 93.5% at an AgNP concentration of 0.55 mg/mL.In summary, AgNPs synthesized using Forsythia suspensa leaf extract demonstrated high catalytic degradation, antibacterial, and antioxidant activities, suggesting their potential applications in wastewater treatment, medical bioactive materials, and other fields.
PAN Tingting
,
WANG Rui
,
ZHOU Wuqiang
,
ZHANG Wenli
,
ZHAO Yanyan
. Optimization and activity evaluation of green synthesis of silver nanoparticles using Forsythia suspensa leaf extract[J]. Food and Fermentation Industries, 2025
, 51(11)
: 244
-252
.
DOI: 10.13995/j.cnki.11-1802/ts.040363
[1] YE M Q, YANG W W, ZHANG M X, et al.Biosynthesis, characterization, and antifungal activity of plant-mediated silver nanoparticles using Cnidium monnieri fruit extract[J].Frontiers in Microbiology, 2023, 14:1291030.
[2] TAKCI D K, GENC S, TAKCI H A M.Cinnamon-based rapid biosynthesis of silver nanoparticles;its characterization and antibacterial properties[J].Journal of Crystal Growth, 2023, 623:127416.
[3] DEVASVARAN K, ALALLAM B, YUNUS M A, et al.Microwave-assisted green synthesis of silver nanoparticles using alkaline extracted crude polysaccharide of C.Nutans:Optimisation, characterisation, toxicity, anticancer potential and antibacterial studies[J].Journal of Drug Delivery Science and Technology, 2023, 86:104688.
[4] 苟琴, 张振.臭常山内生真菌绿色合成纳米银的优化、表征及催化活性研究[J].食品与发酵工业, 2023, 49(23):149-155.
GOU Q, ZHANG Z.Optimization, characterization, and catalytic activity of green synthesis of silver nanoparticles by endophytic fungus from Orixa japonica[J].Food and Fermentation Industries, 2023, 49(23):149-155.
[5] 姚平. 植物多酚纳米银的绿色制备及其对合成染料的催化降解[D].苏州:苏州大学, 2019.
YAO P.Green synthesis of silver nanoparticles by plant-intermediate polyphenols and their catalytic degradation on synthetic dyes[D].Suzhou:Soochow University, 2019.
[6] ANIS S N S, LIEW W C, MOHD MARSIN A, et al.Microwave-assisted green synthesis of silver nanoparticles using pineapple leaves waste[J].Cleaner Engineering and Technology, 2023, 15:100660.
[7] 王学方. 连翘叶化学成分的提取分离及抗氧化和抗肿瘤作用研究[D].郑州:河南农业大学, 2022.
WANG X F.Studies on extraction and isolation of chemical constituents from Forsythia Suspensa leaves and their antioxidant and anti-tumor effects[D].Zhengzhou:Zhengzhou University, 2022.
[8] 田淑芳, 韩旭, 谭奥飞, 等.一测多评法同时测定连翘和连翘叶中多种成分[J].河南大学学报(医学版), 2024, 43(2):96-102.
TIAN S F, HAN X, TAN A F, et al.Simultaneous quantitative determination of 7 components in Forsythiae Fructus and Forsythia suspense leaves by QAMS method[J].Journal of Henan University (Medical Science), 2024, 43(2):96-102.
[9] WANG D H, WANG M Y, SHEN W H, et al.Analysis of chemical compounds and toxicological evaluation of Forsythia suspensa leaves tea[J].Food Science and Biotechnology, 2021, 30(2):305-314.
[10] LI J, QIN Q, ZHA S H, et al.Green extraction of forsythoside A, phillyrin and phillygenol from Forsythia suspensa leaves using a β-cyclodextrin-assisted method[J].Molecules, 2022, 27(20):7055.
[11] LIU Y R, YANG L, WANG J Z, et al.New lignans and phenylethanoid with antioxidant activity from aerial parts of Forsythia suspensa (Thunb.) Vahl[J].Natural Product Research, 2023, 37(5):725-733.
[12] GUO J, TANG J K, WANG B F, et al.Phillygenin from Forsythia suspensa leaves exhibits analgesic potential and anti-inflammatory activity in carrageenan-induced paw edema in mice[J].Journal of Food Biochemistry, 2022, 46(12):e14460.
[13] 程体艳, 张振.菊苣叶提取物绿色合成纳米银的表征及其生物活性研究[J].食品与发酵工业, 2024, 50(15):119-125.
CHENG T Y, ZHANG Z.Characterization and bioactivity of green synthesis nano-silver from chicory leaf extract[J].Food and Fermentation Industries, 2024, 50(15):119-125.
[14] KONDURI V V, KALAGATUR N K, GUNTI L, et al.Green synthesis of silver nanoparticles from Hibiscus tiliaceus L. leaves and their applications in dye degradation, antioxidant, antimicrobial, and anticancer activities[J].South African Journal of Botany, 2024, 168:476-487.
[15] 孟繁茵, 杨振.探讨高效促进种子萌发的新策略[J].天津科技, 2014, 41(5):55-58.
MENG F Y, YANG Z.On new strategy of promoting seed germination with high efficiency[J].Tianjin Science & Technology, 2014, 41(5):55-58.
[16] CHAUHAN P, KUMARI M, CHAUDHARY S, et al.Seed germination studies on Chickpeas, Barley, Mung beans and Wheat with natural biomass and plastic waste derived C-dots[J].Science of The Total Environment, 2022, 837:155593.
[17] SUN Y H, WANG W J, ZHENG F Y, et al.Phytotoxicity of iron-based materials in mung bean:Seed germination tests[J].Chemosphere, 2020, 251:126432.
[18] 邱泽奎. 白芨提取物生物合成纳米银及其生物活性研究[D].福州:福建农林大学, 2022.
QIU Z K.Biosynthesis of silver nanoparticles using bletilla striata extract and its biological activities[D].Fuzhou:Fujian Agriculture and Forestry University, 2022
[19] KHATUN M, KHATUN Z, KARIM M R, et al.Green synthesis of silver nanoparticles using extracts of Mikania cordata leaves and evaluation of their antioxidant, antimicrobial and cytotoxic properties[J].Food Chemistry Advances, 2023, 3:100386.
[20] SMIRNOV O, DZHAGAN V, YESHCHENKO O, et al.Effect of pH of Ganoderma lucidum aqueous extract on green synthesis of silver nanoparticles[J].Advances in Natural Sciences:Nanoscience and Nanotechnology, 2023, 14(3):035009.
[21] HAJIZADEH M, SADEGHIAN SARAYAN M, TALEGHANI A, et al.Evaluation of antimicrobial and antioxidant effects of silver nanoparticles synthesized with leaves of Lepidium draba L[J].Journal of Radiation Research and Applied Sciences, 2024, 17(3):101004.
