Food and Fermentation Industries >

2025 , Vol. 51 >Issue 12: 346 - 351

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041410

Determination of 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry

  • LIU Xiaofang ,
  • JIANG Junjie ,
  • SHI Pengtu ,
  • HE Hao ,
  • TIAN Xiong ,
  • GUO Dan ,
  • LI Xin ,
  • CHEN Yuanyuan
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  • (Hunan Provincial Institute of Product and Goods Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha 410007, China)

Received date: 2024-10-24

  Revised date: 2024-12-24

  Online published: 2025-07-11

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Abstract

An analytical method for the determination of 4-hydroxychlorothalonil residues in animal-derived food by solid phase extraction purification combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established in this study.1% acetic acid acetonitrile solution was used to extract the samples by oscillation.The samples were purified by neutral alumina solid phase extraction column, defatted with n-hexane, and separated by Waters BEH C18 column under a gradient elution program using methanol and 1 mmol/L ammonium acetate aqueous solution as mobile phase.Electrospray ionization source negative ion was used to detect which mode was multiple reaction monitoring mode.The external standard method was selected to quantify.Results showed that the linear relationship of 4-hydroxychlorothalonil was good in the range of 5-500 μg/L when the correlation coefficient (r2) was 0.999 4.The limit of quantitation was determined to be 0.005 mg/kg.When pork, beef, mutton, chicken, chicken skin, pork loin, lard, chicken liver, and milk were used as the substrates, the average recoveries were between 76.9% and 104.7% under three different addition levels.At the same time, the relative standard deviations of 4-hydroxychlorothalonil were 0.6%-4.6%.The pretreatment method is simple and easy to operate, the result is not only accurate but also reliable.Therefore, this analytical method is suitable for the quantitative determination of 4-hydroxychlorothalonil in animal-derived food, which is helpful for the market supervision of 4-hydroxychlorothalonil residues in animal-derived food.

Key words： ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); 4-hydroxychlorothalonil; animal-derived food; solid phase extraction

Cite this article

LIU Xiaofang , JIANG Junjie , SHI Pengtu , HE Hao , TIAN Xiong , GUO Dan , LI Xin , CHEN Yuanyuan . Determination of 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2025 , 51(12) : 346 -351 . DOI: 10.13995/j.cnki.11-1802/ts.041410

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