The probiotic Massa Medicata Fermentata was prepared through intensive fermentation using Lactiplantibacillus plantarum (CGMCC 29919), Lacticaseibacillus rhamnosus (LH-1), and Lactobacillus acidophilus (LA-1).The effectiveness components were determined and the effects on functional dyspepsia mice were investigated.Results showed that the contents of ferulic acid, rutin, quercitrin, quercetin, and luteolin in the prepared probiotic Massa Medicata Fermentata were increased by 35.98%, 191.91%, 241.93%, 65.25%, and 50.08% respectively.The evaluation of the improvement effect on functional dyspepsia model mice showed that compared with the model group, the gastric emptying rate was increased by 46.26% (P<0.01), and the small intestinal propulsive rate was increased by 93.50% (P<0.01), which effectively improved gastrointestinal dynamics and significantly reduced serum IL-4 (69.31 pg/mL) and IL-1β (86.81 pg/mL) levels.Furthermore, it could also promote digestion by regulating hormone levels, inhibiting the production of cholecystokinin and promoting the release of gastric motility.Meanwhile, it was found that the probiotic Massa Medicata Fermentata improved the structure of the intestinal flora mice, and made the abundance of Lachnospiraceae, Akkermansia, and Lactobacillus increased significantly, while the abundance of harmful bacteria Ruminococcus and Helicobacter decreased significantly.Results showed that probiotics-enhanced fermentation of Massa Medicata Fermentata could significantly improve the functional components of Massa Medicata Fermentata, and improve the treatment effect of clinical functional dyspepsia by improving the structure of intestinal flora, regulating the level of gastrointestinal hormones, enhancing gastrointestinal motility, promoting digestion, and reducing inflammatory reaction.
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