Assessing the functional characteristics and safety of probiotics is essential for their application.This study systematically investigated the genomic features, safety, and probiotic properties of Bifidobacterium longum subsp.infantis CICC 6069T and established a rapid strain-level identification method.Whole-genome analysis revealed abundant genes related to carbohydrate and amino acid metabolism, as well as those associated with antioxidant activity, human milk oligosaccharides (HMOs) metabolism, and phytic acid degradation, highlighting its potential as a functional probiotic.Safety evaluation showed that this strain is sensitive to common antibiotics, exhibits no hemolytic activity, and has no acute toxicity, confirming its suitability for probiotic use.Probiotic property assessments demonstrated strong adhesion ability, high DPPH free radical scavenging activity, and significant phytase activity.It effectively promoted iron absorption in intestinal cells, with genomic and in vitro analyses supporting its potential for enhancing mineral uptake.A strain-specific identification method was developed using primers targeting the lipoprotein-encoding gene 6069GL001191, providing an advanced tool for intellectual property protection and quality control.This study comprehensively explored the functionality, safety, and industrial applications of the strain, offering a solid theoretical foundation for its use in gut health regulation and innovation in the probiotic industry.
LI Junfei
,
SONG Zhiquan
,
HE Xiaorui
,
CHEN Yawei
,
GUO Lizheng
,
YU Rui
,
LIU Jiatong
,
GE Yuanyuan
,
CAI Shunfeng
,
YAO Su
. Study on probiotic properties of Bifidobacterium longum subsp.infantis CICC 6069T and development of molecular identification methods[J]. Food and Fermentation Industries, 2025
, 51(14)
: 273
-282
.
DOI: 10.13995/j.cnki.11-1802/ts.042360
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