This study focused on the mechanical and artificial high-temperature Daqu and used high-throughput sequencing technology to compare and analyze the fungal communities contained in it.According to the analysis of α diversity, it could be concluded that compared with artificial high-temperature Daqu, the richness and diversity of fungal communities contained in the mechanical high-temperature Daqu were significantly lower (P<0.01).Through permutation multivariate analysis of variance, it could be concluded that there were significant differences in the composition of fungal communities between the two types of high-temperature Daqu samples (P<0.01).By analyzing the fungal community, it was found that the contents of Symbiotaphrina, Xeromyces, and Byssochlamys in the mechanical high-temperature Daqu were significantly higher (P<0.05), with relative contents of 9.51%, 3.52%, and 0.90% in the mechanical high-temperature Daqu, and 0.02%, 0.02%, and 0.00% in the artificial high-temperature Daqu, respectively.However, the content of Rasamsonia and Thermoascus genera in the artificial high-temperature Daqu was significantly higher (P<0.05), with relative contents of 0.01% and 5.67% in the mechanical high-temperature Daqu, and 1.47% and 27.82% in the artificial high-temperature Daqu, respectively.Through the analysis of operational taxonomic units (OTUs), it was found that 1 569 and 3 727 OTUs were only present in the fungal communities of the mechanical and artificial high-temperature Daqu, respectively, accounting for only 0.86% and 1.58% of the total sequence number, while 8 305 OTUs were simultaneously present in several samples of the two types of high-temperature Daqu, accounting for 97.56% of the total sequence number.Through co-occurrence network analysis, it could be concluded that the average degrees of the co-occurrence networks for mechanical and artificial high-temperature Daqu were 4.034 and 2.718, respectively.From this, it could be seen that there were differences in the fungal community structure and the interaction relationship between fungal genera in the mechanical and artificial high-temperature Daqu, but the differences in fungal communities were mainly reflected in species with lower content.At the same time, compared with the artificial high-temperature Daqu, the stability between fungal genera in the mechanism was higher.
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