Food and Fermentation Industries >

2025 , Vol. 51 >Issue 13: 17 - 28

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040937

Whole gene analysis and enzyme production conditions optimization of Serratia marcescens EC-011

  • SHENG Xinhe ,
  • ZHANG Zhi ,
  • JI Shuai ,
  • WANG Mengai ,
  • ZHANG Xiaotong ,
  • SUN Jiajia ,
  • ZHENG Ying ,
  • LIU Jiansheng ,
  • ZHANG Shenglong
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  • 1(College of Life Sciences, Northeast Forestry University, Harbin 150040, China)
    2(College of Food Engineering, East University of Heilongjiang, Harbin 150076, China)
    3(Heilongjiang Guohong Energy Conservation and Environmental Protection Co.Ltd., Harbin 150040, China)

Received date: 2024-09-02

  Revised date: 2024-10-16

  Online published: 2025-08-04

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Abstract

To better solve the problem of waste of straw lignocellulose resources and provide a biological information basis and theoretical basis for lignocellulose degrading bacterial resources.This study screened and isolated a bacterium of Serratia marcescens EC-011 with strong ability to decompose lignocellulose using Congo red staining and enzyme activity as the evaluation index.And perform whole genome sequencing analysis and optimize enzyme production conditions on it.Its genome is a circular closed DNA with a size of 4 158 647 bp, and 3 398, 2 573, 2 279, and 147 functional genes were annotated in COG, GO, KEGG, and CAZY databases, respectively.AntiSMASH and secretion system analysis revealed diverse secretion mechanisms that may be related to lignocellulose degradation.The optimal enzyme production conditions after single factor and response surface optimization were fermentation temperature of 35 ℃, inoculation amount of 2%, initial pH value of 6.5, and cultivation time of 70 h.After optimization, the enzyme activity of filter paper reached 2.19 U/mL, which was about 1.61 times higher than before optimization.The results indicate that the viscous Sandrella EC-011 has a genetic basis for efficient degradation of lignocellulose, and has potential application value in the biotransformation and energy utilization of lignocellulose.

Key words： Serratia marcescens; lignocellulose; cellulase; whole genome analysis; enzyme production optimization

Cite this article

SHENG Xinhe , ZHANG Zhi , JI Shuai , WANG Mengai , ZHANG Xiaotong , SUN Jiajia , ZHENG Ying , LIU Jiansheng , ZHANG Shenglong . Whole gene analysis and enzyme production conditions optimization of Serratia marcescens EC-011[J]. Food and Fermentation Industries, 2025 , 51(13) : 17 -28 . DOI: 10.13995/j.cnki.11-1802/ts.040937

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