Advances in nanozyme-based electrochemical sensing technology for food safety detection

  • ZHANG Jiahao ,
  • HUANG Chuolin ,
  • LIU Yingju
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  • (College of Materials & Energy, South China Agricultural University, Guangzhou 510642, China)

Received date: 2024-11-14

  Revised date: 2024-12-20

  Online published: 2025-08-04

Abstract

The frequent occurrence of food safety incidents can adversely affect the public’s dietary health, which has led to the increasing demand for food safety detection methods.Nanozymes exhibit excellent catalytic activity due to their ability to catalyze substrates of natural enzyme based on specific nanostructures, which makes them play an important role in electrochemical sensing detection.Therefore, the four catalytic activities of nanozymes (oxidase-like, peroxidase-like, catalase-like, and superoxide dismutase-like) were introduced, the mechanism of nanozymes-catalyzed reactions were elaborated, and the researches on nanozymes-based electrochemical biosensors for food safety detection, including food additives, pesticides, heavy metal ions, foodborne pathogens, and mycotoxins, were summarized.In addition, the existing problems in this field are pointed out, as well as the future development prospects of this field are looked forward.

Cite this article

ZHANG Jiahao , HUANG Chuolin , LIU Yingju . Advances in nanozyme-based electrochemical sensing technology for food safety detection[J]. Food and Fermentation Industries, 2025 , 51(13) : 408 -419 . DOI: 10.13995/j.cnki.11-1802/ts.041594

References

[1] 邓澄. 提高食品安全检验检测质量的意义与路径[J].中国食品, 2024(2):62-64.
DENG C.The significance and path to improve the quality of food safety inspection and testing[J].China Food, 2024(2):62-64.
[2] 王海燕. 食品添加剂应用现状和监管措施研究[J].食品安全导刊, 2024(28):20-22.
WANG H Y.Research on application status of food additives and regulatory measures[J].China Food Safety Magazine, 2024(28):20-22.
[3] 朱蕾, 刘静, 徐欢, 等.植物基食品安全风险及应对策略[J].食品科学, 2024, 45(20):376-386.
ZHU L, LIU J, XU H, et al.Plant-based foods:Safety risks and countermeasures[J].Food Science, 2024, 45(20):376-386.
[4] SI L X, WU Q, JIN Y L, et al.Research progress in the detection of trace heavy metal ions in food samples[J].Frontiers in Chemistry, 2024, 12:1423666.
[5] 刘婷宇, 董庆利, 李嘉铭, 等.食品中食源性致病菌活菌检测方法研究进展[J/OL].食品工业科技, 2024.DOI:10.13386/j.issn1002-0306.2024070120.
LIU T Y, DONG Q L, LI J M, et al.Research progress of detection technology of viable foodborne pathogens[J/OL].Science and Technology of Food Industry, 2024.DOI:10.13386/j.issn1002-0306.2024070120.
[6] 纪文华, 李雪梅, 尹小燕, 等.黄曲霉毒素检测方法研究进展[J].河南工业大学学报(自然科学版), 2022, 43(5):14-21.
JI W H, LI X M, YIN X Y, et al.Research progress of aflatoxin detection methods[J].Journal of Henan University of Technology (Natural Science Edition), 2022, 43(5):14-21.
[7] 国家卫健委印发“十四五”食品安全标准与监测评估规划[J].中国食品工业, 2022(16):12.
National Health Commission issues ‘14th Five-Year Plan’ for food safety standards and monitoring and evaluation[J].China Food Industry, 2022(16):12.
[8] 胡秀智. 原子吸收光谱法在食品金属检测中的研究进展[J].现代食品, 2021, 27(9):20-22.
HU X Z.Research progress of atomic absorption spectrometry in the detection of metals in food[J].Modern Food, 2021, 27(9):20-22.
[9] 黄红滇. 高效液相色谱技术在食品检测中的应用[J].中国食品工业, 2024(21):90-92.
HUANG H D.Application of high performance liquid chromatography in food detection[J].China Food Industry, 2024(21):90-92.
[10] 谢希杨, 黄天然, 黄明.基于碳点的荧光适配体传感器在食品安全检测中的应用研究进展[J].食品科学, 2024, 45(22):361-372.
XIE X Y, HUANG T R, HUANG M.Research progress in the application of carbon dot-based fluorescent aptamer sensors in food safety detection[J].Food Science, 2024, 45(22):361-372.
[11] 宋菁景, 刘雪, 林小晖.色谱-质谱联用技术在食品检测中的应用[J].食品安全导刊, 2022(17):180-183.
SONG J J, LIU X, LIN X H.Application of chromatography-mass spectrometry in food detection[J].China Food Safety Magazine, 2022(17):180-183.
[12] WU J, LIU H, CHEN W W, et al.Device integration of electrochemical biosensors[J].Nature Reviews Bioengineering, 2023, 1(5):346-360
[13] 范克龙, 高利增, 魏辉, 等.纳米酶[J].化学进展, 2023, 35(1):1-87.
FAN K L, GAO L Z, WEI H, et al.Nanozymes[J].Progress in Chemistry, 2023, 35(1):1-87.
[14] 李芙蓉, 向发椿, 曹丽萍, 等.纳米酶在食品检测中的应用研究进展[J].食品科学, 2022, 43(1):285-297.
LI F R, XIANG F C, CAO L P, et al.Recent advances in applications of nanozymes in food detection[J].Food Science, 2022, 43(1):285-297.
[15] CHEN J Q, LIU X Y, ZHENG G C, et al.Detection of glucose based on noble metal nanozymes:Mechanism, activity regulation, and enantioselective recognition[J].Small, 2023, 19(8):2205924.
[16] ZHANG X J, LIN S J, LIU S W, et al.Advances in organometallic/organic nanozymes and their applications[J].Coordination Chemistry Reviews, 2021, 429:213652.
[17] DAI X H, LIU H, DU W X, et al.Biocompatible carbon nitride quantum dots nanozymes with high nitrogen vacancies enhance peroxidase-like activity for broad-spectrum antibacterial[J].Nano Research, 2023, 16(5):7237-7247.
[18] ZHANG X L, LI G L, WU D, et al.Recent progress in the design fabrication of metal-organic frameworks-based nanozymes and their applications to sensing and cancer therapy[J].Biosensors and Bioelectronics, 2019, 137:178-198.
[19] CHEN H Y, YANG T, LIU F Q, et al.Electrodeposition of gold nanoparticles on Cu-based metal-organic framework for the electrochemical detection of nitrite[J].Sensors and Actuators B:Chemical, 2019, 286:401-407.
[20] LIU L, DU J, LIU W E, et al.Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite[J].Analytical and Bioanalytical Chemistry, 2019, 411(10):2189-2200.
[21] CHEN Z C, LAI G S, LIU S, et al.Ultrasensitive electrochemical aptasensing of kanamycin antibiotic by enzymatic signal amplification with a horseradish peroxidase-functionalized gold nanoprobe[J].Sensors and Actuators B:Chemical, 2018, 273:1762-1767.
[22] WU J, WANG X Y, WANG Q, et al.Nanomaterials with enzyme-like characteristics (nanozymes):Next-generation artificial enzymes (II)[J].Chemical Society Reviews, 2019, 48(4):1004-1076.
[23] LI S Q, LIU X D, CHAI H X, et al.Recent advances in the construction and analytical applications of metal-organic frameworks-based nanozymes[J].TrAC Trends in Analytical Chemistry, 2018, 105:391-403.
[24] ZHANG L, WANG H, QU X G.Biosystem-inspired engineering of nanozymes for biomedical applications[J].Advanced Materials, 2024, 36(10):2211147.
[25] 张卫丹, 辛嘉英, 贺姣, 等.纳米酶构效关系及其在食品检测中的应用[J].食品安全质量检测学报, 2023, 14(19):78-88.
ZHANG W D, XIN J Y, HE J, et al.Structure-activity relationship of nanozymes and its applications in food detection[J].Journal of Food Safety & Quality, 2023, 14(19):78-88.
[26] GONG L, CHEN Y, BAI X P, et al.Peroxidase-mimicking Pt nanodots supported on polymerized ionic liquid wrapped multi-walled carbon nanotubes for colorimetric detection of hydrogen peroxide and glucose[J].Microchemical Journal, 2021, 163:105872.
[27] ZHU H S, CAI Y, QILENG A R, et al.Template-assisted Cu2O@Fe(OH)3 yolk-shell nanocages as biomimetic peroxidase:A multi-colorimetry and ratiometric fluorescence separated-type immunosensor for the detection of ochratoxin A[J].Journal of Hazardous Materials, 2021, 411:125090.
[28] LOU Z P, ZHAO S, WANG Q, et al.N-doped carbon as peroxidase-like nanozymes for total antioxidant capacity assay[J].Analytical Chemistry, 2019, 91(23):15267-15274.
[29] WU G J, DILINAER A, NIE P, et al.Dual-modal bimetallic nanozyme-based sensing platform combining colorimetric and photothermal signal cascade catalytic enhancement for detection of hypoxanthine to judge meat freshness[J].Journal of Agricultural and Food Chemistry, 2023, 71(43):16381-16390.
[30] LI Y C, WANG P, HUANG L J, et al.Schiff-base chemistry-coupled catechol oxidase-like nanozyme reaction as a universal sensing mode for ultrasensitive biosensing[J].Analytical Chemistry, 2023, 95(7):3769-3778.
[31] ZHANG J Y, ZHONG Y J, ZHANG C Y, et al.Mesoporous core-shell Pd@Pt nanospheres as oxidase mimics with superhigh catalytic efficiency at room temperature[J].The Journal of Physical Chemistry Letters, 2022, 13(9):2137-2143.
[32] CHENG H, CHEN Y Y, LIU M J, et al.Theory-guided design of S-doped Fe/Co dual-atom nanozymes for highly efficient oxidase mimics[J].Chemical Science, 2024, 15(36):14816-14828.
[33] GEBICKA L, KRYCH-MADEJ J.The role of catalases in the prevention/promotion of oxidative stress[J].Journal of Inorganic Biochemistry, 2019, 197:110699.
[34] CHEN M T, QIU Q Q, QILENG A R, et al.Efficient nanozyme-triggered pressure sensor for point-of-care immunoassay:Visual sensing and time readout device[J].Analytical Chemistry, 2023, 95(30):11383-11390.
[35] QIAO Q Q, WANG J Y, LONG K, et al.A cascaded enzyme system based on the catalase-like activity of Ti3C2Tx MXene nanosheets for the efficient combination cancer therapy[J].Nano Today, 2024,54:102059.
[36] LI H, XIA X Y, ZANG J C, et al.Construction of manganese-based oyster (Crassostrea gigas) ferritin nanozyme with catalase-like enzyme activity[J].Journal of Agricultural and Food Chemistry, 2024, 72(1):810-818.
[37] SAHOO B M, BANIK B K, BORAH P, et al.Reactive oxygen species (ROS):Key components in cancer therapies[J].Anti-Cancer Agents in Medicinal Chemistry, 2022, 22(2):215-222.
[38] WU H Y, BU T, CAO Y Y, et al.Double-enzyme active vanadium nanospheres-mediated ratiometric multicolor immunosensors for sensitive detection of the T-2 toxin[J].Analytical Chemistry, 2023, 95(12):5275-5284.
[39] ZHANG Y J, GAO W H, MA Y N, et al.Integrating Pt nanoparticles with carbon nanodots to achieve robust cascade superoxide dismutase-catalase nanozyme for antioxidant therapy[J].Nano Today, 2023, 49:101768.
[40] APARNA, GARG M, VISHWAKARMA N, et al.Molecularly imprinted conducting polymer based sensor for Salmonella typhimurium detection[J].Bioelectrochemistry, 2022, 147:108211.
[41] ZHANG J L, WANG J J, ZHANG X Q, et al.Rapid detection of Escherichia coli based on 16S rDNA nanogap network electrochemical biosensor[J].Biosensors and Bioelectronics, 2018, 118:9-15.
[42] ANGELOPOULOU M, KOURTI D, MERTIRI M, et al.A 3D-printed electrochemical immunosensor employing Cd/Se ZnS QDs as labels for the rapid and ultrasensitive detection of Salmonella typhimurium in poultry samples[J].Chemosensors, 2023, 11(9):475.
[43] 张玉, 宋志敏, 杜衍.纳米酶传感器在现场即时检测领域的应用进展[J].分析化学, 2023, 51(5):800-810.
ZHANG Y, SONG Z M, DU Y.Recent progress of nanozyme-based sensors in point-of-care testing[J].Chinese Journal of Analytical Chemistry, 2023, 51(5):800-810.
[44] LI C, ZHANG X N, TANG Q Y, et al.Molecularly imprinted electrochemical sensor for ethyl carbamate detection in Baijiu based on “on-off” nanozyme-catalyzing process[J].Food Chemistry, 2024, 453:139626.
[45] GE F G, SUN Y J, WANG K, et al.Colorimetric/electrochemical dual mode detection ascorbic acid based Au@PdNi nanozyme[J].Microchemical Journal, 2024, 201:110745.
[46] SHI X Y, LI J, XIONG Y, et al.Rh single-atom nanozymes for efficient ascorbic acid oxidation and detection[J].Nanoscale, 2023, 15(14):6629-6635.
[47] WANG M Z, ZHAO M H, LIU P, et al.Coupling diazotization with oxidase-mimetic catalysis to realize dual-mode double-ratiometric colorimetric and electrochemical sensing of nitrite[J].Sensors and Actuators B:Chemical, 2022, 355:131308.
[48] GUAN H N, XING K, LIU S P.Green synthesis of Au magnetic nanocomposites using waste chestnut skins and their application as a peroxidase mimic nanozyme electrochemical sensing platform for sodium nitrite[J].Foods, 2023, 12(19):3665.
[49] KONG L Q, HONG F, LUAN P, et al.Novel competitive electrochemical impedance biosensor for the ultrasensitive detection of umami substances based on Pd/Cu-TCPP(Fe)[J].Food Chemistry, 2024, 438:137631.
[50] QILENG A R, LIU W P, LIANG H Z, et al.Tuning the electronic configuration of oxygen atom in engineering non-self-limited nanozyme for portable immunosensor[J].Advanced Functional Materials, 2024, 34(10):2311783.
[51] CHEN M T, QILENG A R, CHEN S Z, et al.Advanced enzyme mimicking engineering:3D biomimetic Pt single-atom nanozymes initiating pressure-driven device[J].Advanced Functional Materials, 2024, 34(38):2402552.
[52] LI Y, ZHANG Y Y, JAVED R, et al.Nonmetal catalyst boosting amplification of both colorimetric and electrochemical signal for multi-mode nitrite sensing[J].Food Chemistry, 2024, 441:138315.
[53] JIANG L Y, HU R, WANG A J, et al.1D/2D SnO2/SnS2 heterojunctions coupling with PtPd/CeO2 heterostructured nanozyme for ultrasensitive PEC apatasensing of lincomycin[J].Sensors and Actuators B:Chemical, 2023, 382:133491.
[54] LI S H, MA X H, PANG C H, et al.Novel chloramphenicol sensor based on aggregation-induced electrochemiluminescence and nanozyme amplification[J].Biosensors and Bioelectronics, 2021, 176:112944.
[55] ZHAO F, GUO D Q, TANG X, et al.Ratiometrically electrochemical and colorimetric dual-mode detection of glyphosate based on 2D Cu-TCPP(Fe) NSs[J].Talanta, 2024, 267:125207.
[56] WEN S H, ZHANG H Y, YU S, et al.Nanozyme coating-gated multifunctional COF composite based dual-ratio enhanced dual-mode sensor for highly sensitive and reliable detection of organophosphorus pesticides in real samples[J].Journal of Hazardous Materials, 2024, 480:135791.
[57] LI S H, PANG C H, MA X H, et al.Microfluidic paper-based chip for parathion-methyl detection based on a double catalytic amplification strategy[J].Mikrochimica Acta, 2021, 188(12):438.
[58] QIU L H, LV P, ZHAO C L, et al.Electrochemical detection of organophosphorus pesticides based on amino acids conjugated nanoenzyme modified electrodes[J].Sensors and Actuators B:Chemical, 2019, 286:386-393.
[59] YU L, CHANG J F, ZHUANG X Y, et al.Two-dimensional cobalt-doped Ti3C2 MXene nanozyme-mediated homogeneous electrochemical strategy for pesticides assay based on in situ generation of electroactive substances[J].Analytical Chemistry, 2022, 94(8):3669-3676.
[60] ZHU X Y, LIN L, WU R M, et al.Portable wireless intelligent sensing of ultra-trace phytoregulator α-naphthalene acetic acid using self-assembled phosphorene/Ti3C2-MXene nanohybrid with high ambient stability on laser induced porous graphene as nanozyme flexible electrode[J].Biosensors and Bioelectronics, 2021, 179:113062.
[61] WANG G X, LIU J, DONG H W, et al.A dual-mode biosensor featuring single-atom Fe nanozyme for multi-pesticide detection in vegetables[J].Food Chemistry, 2024, 437:137882.
[62] ZENG H L, CHEN H L, YANG B, et al.Highly-oxidizing Au@MnO2-X nanozymes mediated homogeneous electrochemical detection of organophosphorus independent of dissolved oxygen[J].Journal of Hazardous Materials, 2023, 459:132116.
[63] WANG R Y, LI B B, LI G P, et al.NiCoFeS/rGO nanozyme-mediated multifunctional homogeneous sensing system for ultrasensitive electrochemical assay of pesticides residues in fruits and vegetables[J].Sensors and Actuators B:Chemical, 2025, 422:136664.
[64] DIAO Q Q, BU Z J, FENG R L, et al.Performance-complementary colorimetric/electrochemical bimodal detection of Hg2+ based on analyte-accelerated peroxidase-mimicking activity of GO-AuNPs[J].Sensors and Actuators B:Chemical, 2025, 422:136598.
[65] LIU T T, ZHOU R Y, WU K C, et al.Colorimetric method transforms into highly sensitive homogeneous voltammetric sensing strategy for mercury ion based on mercury-stimulated Ti3C2Tx MXene nanoribbons@gold nanozyme activity[J].Analytica Chimica Acta, 2023, 1250:340975.
[66] NATARAJ N, DASH P, SAKTHIVEL R, et al.Simultaneous electrochemical and colorimetric detection of tri-heavy metal ions in environmental water samples employing 3D-MOF/nickel selenide as a synergistic catalyst[J].Chemical Engineering Journal, 2024, 485:149965.
[67] HUANG X W, HUANG C Y, ZHOU L L, et al.Allosteric switch for electrochemical aptasensor toward heavy metals pollution of Lentinus edodes sensitized with porphyrinic metal-organic frameworks[J].Analytica Chimica Acta, 2023, 1278:341752.
[68] ZHANG C L, YI Y H, ZHOU Y F, et al.Homogeneous electroanalysis coupled with colorimetry dual-mode sensing of silver ion in water based on target-inhibited peroxidase activity of Pt@ZIF-8 nanozyme[J].Microchemical Journal, 2024, 197:109839.
[69] YANG Q Y, WAN C Q, WANG Y X, et al.Bismuth-based metal-organic framework peroxidase-mimic nanozyme:Preparation and mechanism for colorimetric-converted ultra-trace electrochemical sensing of chromium ion[J].Journal of Hazardous Materials, 2023, 451:131148.
[70] WANG W H, XIAO S, ZENG M L, et al.Dual-mode colorimetric-electrochemical biosensor for Vibrio parahaemolyticus detection based on CuO2 nanodot-encapsulated metal-organic framework nanozymes[J].Sensors and Actuators B:Chemical, 2023, 387:133835.
[71] JIANG X H, LV Z W, RAO C Q, et al.Simple and highly sensitive electrochemical detection of Listeria monocytogenes based on aptamer-regulated Pt nanoparticles/hollow carbon spheres nanozyme activity[J].Sensors and Actuators B:Chemical, 2023, 392:133991.
[72] HU W C, PANG J, BISWAS S, et al.Ultrasensitive detection of bacteria using a 2D MOF nanozyme-amplified electrochemical detector[J].Analytical Chemistry, 2021, 93(24):8544-8552.
[73] MA Y X, LIN X H, XUE B, et al.Ultrasensitive and highly selective detection of Staphylococcus aureus at the single-cell level using bacteria-imprinted polymer and vancomycin-conjugated MnO2 nanozyme[J].Analytical Chemistry, 2024, 96(21):8641-8647.
[74] TIAN L, ZHANG Y, WANG L B, et al.Ratiometric dual signal-enhancing-based electrochemical biosensor for ultrasensitive kanamycin detection[J].ACS Applied Materials & Interfaces, 2020, 12(47):52713-52720.
[75] WU C Y, YUE Y Y, HUANG B C, et al.CRISPR-powered microfluidic biosensor for preamplification-free detection of ochratoxin A[J].Talanta, 2024, 269:125414.
[76] WU C Y, WU X C, HOU F, et al.An ultrasensitive electrochemical aptasensor based on Pd@PCN-222 as a signal probe coupled with exonuclease III-assisted cycling amplification for the detection of ochratoxin A[J].Food Control, 2022, 139:109066.
[77] LI Y L, CHEN Y Y, XIE F T, et al.Smartphone-based dual-mode aptasensor with bifunctional metal-organic frameworks as signal probes for ochratoxin A detection[J].Food Chemistry, 2025, 464:141540.
[78] CHEN D N, WANG G Q, MEI L P, et al.Dual II-scheme nanosheet-like Bi2S3/Bi2O3/Ag2S heterostructures for ultrasensitive PEC aptasensing of aflatoxin B1 coupled with catalytic signal amplification by dendritic nanorod-like Au@Pd@Pt nanozyme[J].Biosensors and Bioelectronics, 2023, 223:115038.
[79] WEI J J, WANG G Q, ZHENG J Y, et al.Z-scheme Cu2MoS4/CdS/In2S3 nanocages heterojunctions-based PEC aptasensor for ultrasensitive assay of fumonisin B1 via signal amplification with hollow PtPd-CoSnO3 nanozyme[J].Biosensors and Bioelectronics, 2023, 230:115293.
[80] LI Z Z, WANG Y F, JI X X, et al.Ultrasensitive “signal-inversion” photoelectrochemical aptasensor based on semiconductive MOF integrated with the manganese ferrite nanozyme-regulation for the selective detection of fumonisin B1[J].Sensors and Actuators B:Chemical, 2024, 420:136473.
[81] WEI J, QILENG A R, YAN Y, et al.A novel visible-light driven photoelectrochemical immunosensor based on multi-amplification strategy for ultrasensitive detection of microcystin-LR[J].Analytica Chimica Acta, 2017, 994:82-91.
[82] MEI L P, XU J J, TANJUNG A P, et al.Integration of high-entropy nanozyme and hollow In2S3 nanotube heterostructures decorated with WO3 for ultrasensitive PEC aptasensing of highly toxic mycotoxin[J].Sensors and Actuators B:Chemical, 2024, 414:135952.
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