Acute kidney injury (AKI) is a common clinical renal disease, with inflammation identified a key factor contributing to kidney damage.This study established a cisplatin-induced mouse model of AKI and evaluated the effects of live bacteria, fermented supernatant, and a bacterial lysate of Limosilactobacillus reuteri DYNDL56M17 (a strain with anti-inflammatory properties) on alleviating AKI in mice.Evaluation included examining renal histopathological sections and measuring the levels of -serum creatinine, blood urea nitrogen (BUN), antioxidants, and inflammatory cytokines.Compared to the model group, the fermented supernatant and bacterial lysate of L.reuteri DYNDL56M17 significantly reduced creatinine and BUN levels.Specifically, the fermented supernatant reduced creatinine and BUN levels by 27.9% and 16.7%, respectively, whereas the bacterial lysate reduced these levels by 21.1% and 10.1%, respectively.Additionally, the fermented supernatant effectively reduced oxidative damage in the kidneys, lowering the renal malondialdehyde content by 13.49% and decreasing the levels of inflammatory cytokines.These findings suggest that the fermented supernatant and bacterial lysate of L.reuteri DYNDL56M17 may alleviate AKI by upregulating the G-protein-coupled receptor GPR43 expression, thereby inhibiting inflammation and oxidative stress.
WANG Ziqi
,
ZHANG Qiuxiang
,
ZHAO Jianxin
. Effect and mechanism analysis of Limosilactobacillus reuteri DYNDL56M17 in assisting alleviation of acute kidney injury[J]. Food and Fermentation Industries, 2025
, 51(15)
: 51
-58
.
DOI: 10.13995/j.cnki.11-1802/ts.041170
[1] 闫薛, 缪扣荣.急性白血病患者NF-κB1基因单核苷酸多态性与异基因造血干细胞移植后急性肾损伤的相关性分析[J].药物生物技术, 2023, 30(1):7-11.
YAN X, MIAO K R.The association of single nucleotide polymorphisms in NF-κB1 gene with the risk of acute kidney injury in acute leukemia patients receiving allogeneic hematopoietic stem cell transplantation[J].Pharmaceutical Biotechnology, 2023, 30(1):7-11.
[2] KHWAJA A.KDIGO clinical practice guidelines for acute kidney injury[J].Nephron.Clinical Practice, 2012, 120(4):c179-c184.
[3] PEERAPORNRATANA S, MANRIQUE-CABALLERO C L, GÓMEZ H, et al.Acute kidney injury from sepsis:Current concepts, epidemiology, pathophysiology, prevention and treatment[J].Kidney International, 2019, 96(5):1083-1099.
[4] ANDRADE-OLIVEIRA V, AMANO M T, CORREA-COSTA M, et al.Gut bacteria products prevent AKI induced by ischemia-reperfusion[J].Journal of the American Society of Nephrology, 2015, 26(8):1877-1888.
[5] HU Y M, PAI M H, YEH C L, et al.Glutamine administration ameliorates sepsis-induced kidney injury by downregulating the high-mobility group box protein-1-mediated pathway in mice[J].American Journal of Physiology.Renal Physiology, 2012, 302(1):F150-F158.
[6] LIU Y H.Cellular and molecular mechanisms of renal fibrosis[J].Nature Reviews.Nephrology, 2011, 7(12):684-696.
[7] LIU Y Z, LI Y J, LOH Y W, et al.Fiber derived microbial metabolites prevent acute kidney injury through G-protein coupled receptors and HDAC inhibition[J].Frontiers in Cell and Developmental Biology, 2021, 9:648639.
[8] RAMESH G, RANGANATHAN P.Mouse models and methods for studying human disease, acute kidney injury (AKI)[J].Methods in Molecular Biology, 2014, 1194:421-436.
[9] OZKOK A, EDELSTEIN C L.Pathophysiology of cisplatin-induced acute kidney injury[J].BioMed Research International, 2014, 2014(1):967826.
[10] YANG J, JI G E, PARK M S, et al.Probiotics partially attenuate the severity of acute kidney injury through an immunomodulatory effect[J].Kidney Research and Clinical Practice, 2021, 40(4):620-633.
[11] MENG Y, ZHAO M Z, MA Q Y, et al.Bifidobacterium bifidum alleviates adenine-induced acute kidney injury in mice by improving intestinal barrier function[J].Food & Function, 2024, 15(15):8030-8042.
[12] THOMPSON L E, MCGINNIS C D, EDELSTEIN C L, et al.Cisplatin-induced AKI cancer mouse model refinement[J].Journal of the American Society of Nephrology, 2021, 32(10S):578.
[13] 邱赛红, 李飞艳, 尹健康, 等.9味苦寒药对小鼠胃肠运动与肝肾功能影响的实验研究[J].湖南中医学院学报, 2004, 24(5):1-3;6.
QIU S H, LI F Y, YIN J K, et al.An experimental study on the effects of nine medicinal herbs with the properties of bitterness and cold on gastrointestinal movement and hepatorenal function of mice[J].Journal of Hunan Traditional Chinese Medicine University of Hunan, 2004, 24(5):1-3;6.
[14] MOMBEINI M A, KALANTAR H, SADEGHI E, et al.Protective effects of berberine as a natural antioxidant and anti-inflammatory agent against nephrotoxicity induced by cyclophosphamide in mice[J].Naunyn-Schmiedeberg's Archives of Pharmacology, 2022, 395(2):187-194.
[15] VILLEGAS C V, GORMAN E, LIU F M, et al.Acute kidney injury in the acute care surgery patient:What you need to know[J].The Journal of Trauma and Acute Care Surgery, 2024.
[16] REWA O, BAGSHAW S M.Acute kidney injury-epidemiology, outcomes and economics[J].Nature Reviews.Nephrology, 2014, 10(4):193-207.
[17] HUO A J, WANG F M.Berberine alleviates ischemia reperfusion injury induced AKI by regulation of intestinal microbiota and reducing intestinal inflammation[J].BMC Complementary Medicine and Therapies, 2024, 24(1):66.
[18] FOELSCH K, PELCZAR P, ZIERZ E, et al.Intestinal epithelia and myeloid immune cells shape colitis severity and colorectal carcinogenesis via high-mobility group box protein 1[J].Journal of Crohn's & Colitis, 2024, 18(7):1122-1133.
[19] 孙键. 短链脂肪酸乙酸在脓毒症诱导急性肾损伤中的保护作用及机制研究[D].长春:吉林大学, 2022.
SUN J.Study on the protective effect and mechanism of short-chain fatty acid acetic acid in sepsis-induced acute kidney injury[D].Changchun:Jilin University, 2022.
[20] 虞亘明, 王鑫玮, 骆金光, 等.SPP1在肾缺血再灌注诱导的急性肾损伤中的作用及机制[J].南方医科大学学报, 2023, 43(11):1947-1954.
YU G M, WANG X W, LUO J G, et al.Role of SPP1 in acute kidney injury induced by renal ischemia-reperfusion in rats[J].Journal of Southern Medical University, 2023, 43(11):1947-1954.
