Food and Fermentation Industries >

2025 , Vol. 51 >Issue 15: 66 - 73

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040851

Qualitative and quantitative screening and identification of endophytic ginger bacteria with lipase inhibitory activity

  • PENG Han ,
  • XIE Xinran ,
  • CHEN Siwei ,
  • LI Sixuan ,
  • ZHANG Jiahui ,
  • REN Hong
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  • (School of Light Industry, Beijing Technology and Business University, Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing 100048, China)

Received date: 2024-08-25

  Revised date: 2024-11-11

  Online published: 2025-08-22

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Abstract

Ginger has the effect of inhibiting lipid absorption, lowering blood pressure and preventing atherosclerosis.Endophytic bacteria with lipase inhibitory activity were isolated and purified from ginger slices.Totally six active endophytic bacteria were qualitatively detected by TLC-bioautography, and five strains belonged to Pseudomonas, one belonged to Acinetobacter by 16S rRNA gene sequence comparison.The p-nitrophenyl palmitate method was used to quantitatively analyze the inhibition rate of the 20 mL ethyl acetate extract of six strains of active bacteria on lipase.The incubation time was 5 min, the enzymatic reaction time was 15 min, and the reactants were added in the order of extraction, p-nitrophenyl palmitate, and enzyme.It was found that the inhibition rate of four strains was above 20%, among which SJ14 and SJ12 had higher inhibition rate, reaching (67.79±1.75)% and (48.28±0.73)%, respectively.The active compounds in the extract were detected by HPLC fingerprint.The aim of this study was to establish a new idea of qualitative and quantitative screening of microbial lipase inhibitors, and to provide scientific basis for obtaining potential production strains of highly effective lipid-lowering active substances and highly effective and low-toxic lipase inhibitors.

Key words： ginger; endophytic bacteria; lipase inhibitory activity; qualitative screening; quantitative screening

Cite this article

PENG Han , XIE Xinran , CHEN Siwei , LI Sixuan , ZHANG Jiahui , REN Hong . Qualitative and quantitative screening and identification of endophytic ginger bacteria with lipase inhibitory activity[J]. Food and Fermentation Industries, 2025 , 51(15) : 66 -73 . DOI: 10.13995/j.cnki.11-1802/ts.040851

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