Food and Fermentation Industries >

2025 , Vol. 51 >Issue 15: 241 - 247

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041202

Comparative metabolome analysis and antioxidant activity of triterpenoids from Morchella fruiting bodies

  • ZHAO Wantong ,
  • HE Zhe ,
  • LIU Jing ,
  • YANG Jian ,
  • WANG Hongling ,
  • YANG Yuhong ,
  • CHEN Hongman
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  • 1(College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China)
    2(Shanghai CHANDO Group Co.Ltd., Shanghai 200000, China)

Received date: 2024-10-03

  Revised date: 2024-12-02

  Online published: 2025-08-22

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Abstract

Morchella is a homologous fungus with high nutritional and medicinal value, but there are few studies on the different metabolites of different varieties of Morchella.To compare the differences of triterpenoid metabolites in the fruiting bodies of different varieties of Morchella, UPLC-MS/MS was used to conduct targeted metabolomics analysis of triterpenoids of Morchella sextelata and Morchella importuna and to compare their antioxidant activities.Metabolomic analysis showed that a total of 16 triterpenoid metabolites were detected, among which 8 different triterpenoid metabolites were screened.The contents of hawthoronic acid, corosolic acid, betulinol, and oleanolic acid-3-O-glucoside were relatively higher in M. sextelata, and the contents of 2α,19α-dihydroxyursolic acid, betulinic acid, 11-ketoursolic acid, and 27, 28-dicarboxyursolic acid were relatively higher in M. importuna.Antioxidant activity results showed that the antioxidant activity of M. sextelata was better than that of M. importuna, and the EC50 value of DPPH free radicals were 0.259 mg/mL and 0.396 mg/mL, respectively.The EC50 of superoxide anion free radical was 0.495 mg/mL and 0.895 mg/mL.The EC50 of hydroxyl radical was 0.896 mg/mL and 1.263 mg/mL The total reducing power was 0.86 and 0.81, respectively.The results of this study provide important theoretical reference for the breeding of Morchella and the application of active ingredients in the research and development of new drugs.

Key words： Morchella; fruiting body; triterpenoids; targeted metabolomics; antioxidant activity

Cite this article

ZHAO Wantong , HE Zhe , LIU Jing , YANG Jian , WANG Hongling , YANG Yuhong , CHEN Hongman . Comparative metabolome analysis and antioxidant activity of triterpenoids from Morchella fruiting bodies[J]. Food and Fermentation Industries, 2025 , 51(15) : 241 -247 . DOI: 10.13995/j.cnki.11-1802/ts.041202

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