Rubus chingii Hu is a medicinal and edible plant of the genus Rubus in the family Rosaceae and exhibits good antioxidant and hypoglycaemic activities.This study investigated the effect of plasma-assisted extraction on the phenolics and flavonoids contents, antioxidant and hypoglycaemic activities of R.chingii Hu.The phytochemicals profiling of R.chingii Hu extracts were further identified by mass spectrometry.Results showed that the total phenolics and flavonoids contents of R.chingii Hu respectively increased by 8.26% to 16.08% and 3.03% to 42.59%, the antioxidant and hypoglycaemic activities of it were also significantly enhanced after plasma treatment.The highest total phenolics content (362.5 μg GAE/mg E), DPPH free radical scavenging (IC50=78.38 μg/mL) and α-glucosidase inhibitory (IC50=8.26 μg/mL) abilities were found in the extract of R.chingii Hu pretreated with plasma at 200 W for 5 min.While the extract of R.chingii Hu pre-treated with plasma at 300 W for 5 min exhibited the strongest total flavonoid content (17.41 mg QμE/g E), ABTS cationic radical scavenging (IC50=33.97 μg/mL), and DPP-IV enzyme inhibition (IC50=507.60 μg/mL) acticvities.Fourteen compounds were identified in R.chingii Hu, including gallic acid, (epi)catechin, dihydrokaempferol-hexoside, and ellagic acid.Plasma treatment could increase phenolic acid content, which was the major antioxidant and hypoglycaemic component in R.chingii Hu.Therefore, plasma-assisted extraction could significantly improve the extraction efficiency of polyphenols from R.chingii Hu and enhance its antioxidant and hypoglycaemic activities.The results may provide a theoretical basis for the application of plasma in extracting polyphenols from R.chingii Hu.
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