Mitigating effect of active peptides from shrimp heads on fatigue induced by oxidative stress in exhausted swimming mice

PENG Guangfei; ZHANG Shiqi; WAN Yonglun; LYU Xiaohong; XIE Baoling; ZHOU Zhenxian; XIONG Huan; DENG Tongxing

doi:10.13995/j.cnki.11-1802/ts.042820

2025 , Vol. 51 >Issue 16: 134 - 141

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042820

Mitigating effect of active peptides from shrimp heads on fatigue induced by oxidative stress in exhausted swimming mice

PENG Guangfei ,
ZHANG Shiqi ,
WAN Yonglun ,
LYU Xiaohong ,
XIE Baoling ,
ZHOU Zhenxian ,
XIONG Huan ,
DENG Tongxing

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1(Luohe Medical College, Luohe 462000, China)
2(College of Laboratory of Food Science and Engineering, Lingnan Normal University, Zhanjiang 524048, China)
3(School of Biological&Chemical Engineering, Chongqing University of education, Chongqing 400067, China)

Received date: 2025-03-25

Revised date: 2025-04-08

Online published: 2025-08-29

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Abstract

To investigate the antioxidant stress and anti-fatigue effects of shrimp head active polypeptide (SP) on mice with exhaustive swimming, the DPPH free radical scavenging ability and hydroxyl radical (·OH) scavenging ability of SP were first measured to evaluate its antioxidant performance.Then, mice in each group were gavaged with low, medium, and high doses of SP for 28 consecutive days.Mice exhaustive swimming time, antioxidant related biochemical indices, as well as the mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and cleaved caspase 3 (C-caspase 3) were determined. Results showed that when the mass concentration was 6.0-8.0 mg/mL, the DPPH free radical scavenging rate (83.04%) and ·OH scavenging rate (94.50%) of SP showed optimal scavenging activity.In the animal experiment, the exhaustive swimming time of mice in each dose group of SP was significantly longer than that of the normal blank group (P<0.05).The activities of catalase, total superoxide dismutase, calcium-magnesium adenosine triphosphatase, and sodium-potassium adenosine triphosphatase in the medium and high dose groups of SP were significantly enhanced (P<0.05), and the mRNA expression levels of Nrf2 and HO-1 were significantly upregulated, while the mRNA expression level of C-caspase 3 was significantly downregulated (P<0.05).In conclusion, the results from both in vitro free radical scavenging experiments and animal experiments demonstrated that SP exhibited significant antioxidant effects.It effectively alleviated oxidative stress symptoms in mice while markedly reducing fatigue manifestations.These findings suggested its potential as a novel functional food or anti-fatigue therapeutic agent.

Key words： shrimp head autolytic active peptides; antioxidant effect; Nrf2/HO-1 signaling pathway; exhausted swimming mice; Litopenaeus vannamei

Cite this article

PENG Guangfei , ZHANG Shiqi , WAN Yonglun , LYU Xiaohong , XIE Baoling , ZHOU Zhenxian , XIONG Huan , DENG Tongxing . Mitigating effect of active peptides from shrimp heads on fatigue induced by oxidative stress in exhausted swimming mice[J]. Food and Fermentation Industries, 2025 , 51(16) : 134 -141 . DOI: 10.13995/j.cnki.11-1802/ts.042820

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