Food and Fermentation Industries >

2025 , Vol. 51 >Issue 17: 26 - 32

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041362

Analysis of carbon source utilization and environmental tolerance of Limosilactobacillus reuteri

  • LI Zheyuan ,
  • YANG Yong ,
  • AI Lianzhong ,
  • CUI Bingke ,
  • WANG Chenxu ,
  • SONG Xin
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  • (School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Received date: 2024-10-21

  Revised date: 2024-12-22

  Online published: 2025-09-29

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Abstract

In the domains of food science, agriculture, and medical research, Limosilactobacillus reuteri has attracted significant attention due to its remarkable probiotic attributes.This study aimed to investigate the carbon source fermentation characteristics and environmental adaptability of L.reuteri AR673, providing theoretical guidance for related industries.By modifying the cultivation milieu and carbon source types for AR673, this study ascertained its growth curves and maximum biomass, assessing its response to various stress conditions and its efficiency in utilizing different carbon sources.The findings regarding carbon source utilization revealed that AR673 displayed the highest rate of glucose utilization at 97.91%, with lactose and sucrose following at 92.21% and 89.76%, respectively.In terms of pH tolerance, AR673 exhibited a broad tolerance spectrum spanning from pH 4.0 to 9.0, with optimal growth occurring under neutral to slightly acidic conditions.Furthermore, temperature stress analysis indicated that the optimal growth temperature for AR673 was 37 ℃.AR673 was found to be capable of growth at lower glucose concentrations (5 g/L and 10 g/L), although its maximum biomass was considerably diminished.An increase in glucose concentration facilitated biomass accumulation, with a peak biomass of 2.30±0.052 achieved at a glucose concentration of 50 g/L.Notably, within a specific range, elevated bile salt concentrations positively influenced biomass accumulation in AR673, suggesting that controlled stress could augment the strain's vitality.This study lays a foundational data set for the research on the environmental tolerance of L.reuteri and the optimization of fermentation processes, while also providing a scientific rationale for the potential applications of this strain across various sectors.

Key words： Limosilactobacillus reuteri AR673; growth curve; maximum biomass; carbon source utilization; environmental stress

Cite this article

LI Zheyuan , YANG Yong , AI Lianzhong , CUI Bingke , WANG Chenxu , SONG Xin . Analysis of carbon source utilization and environmental tolerance of Limosilactobacillus reuteri[J]. Food and Fermentation Industries, 2025 , 51(17) : 26 -32 . DOI: 10.13995/j.cnki.11-1802/ts.041362

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