The health effects of probiotics on hosts are closely related to the number of viable bacteria that effectively reach the intestine.Crystal balls with acid-resistant outer layer and three-layer structure have a good digestive protection effect on probiotics, but there are technical difficulties such as poor molding.This study intended to solve the problems of molding and shelf-life stability of probiotic crystal balls and to evaluate the survival rates of probiotics in different dosage forms using biomimetic gastrointestinal digestion model.Comparing crystal balls with powders, the changes in gastric/intestinal pH, crystal balls morphology, and concentration of probiotics during continuous digestion for 3 hours in the dynamic biomimetic digestion model were observed.Results showed that the crystal balls remained intact in an empty stomach environment (pH about 1.6) for 60 minutes, and the survival rates of probiotics remained above 90%.The crystal balls passed through the small intestine (pH about 8.0) at the fastest within 30-60 minutes and gradually dissolved until it completely disappeared after 120 minutes, releasing probiotics encapsulated inside.Under the same digestion conditions, live bacteria of probiotic powders were only detected in the stomach and rapidly decreased, while no live bacteria were detected in the small intestine collector, indicating that the digestive fluid had a significant impact on probiotic activity under an empty stomach.In summary, the crystal balls dosage form has a good protective effect on probiotics, helping to achieve targeted release in the intestine and exerting health benefits.
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