Food and Fermentation Industries >

2025 , Vol. 51 >Issue 17: 195 - 204

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041569

Analysis on existing forms of fluorine and its correlation with microbial population during the pile-fermentation of dark tea

  • GUO Mengyi ,
  • PAN Xueli ,
  • LUO Yan ,
  • LI Chengzhuo ,
  • HU Xianchun ,
  • ZHAO Zhenjun
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  • (College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China)

Received date: 2024-11-12

  Revised date: 2024-12-25

  Online published: 2025-09-29

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Abstract

The study aims to analyze the content of different forms of fluoride and the changes in microbial population during pile-fermentation of tea, and to explore the correlation between the forms of fluoride and the changes in microbial populations during the pile-fermentation of dark tea.Tea samples from 9 different pile-fermentation stages of dark tea were collected to analyze the fluoride content in different existing forms during the pile-fermentation process of dark tea.High-throughput sequencing technology was used to analyze microbial diversities in pile-fermentation, and spearman correlation analysis was used to reveal the correlation between fluoride in different forms and microbial populations.The analysis results of fluorine content in different existing forms of tea samples showed that the general distribution of fluorine content was water-soluble fluorine>organic bound fluorine>exchangeable fluorine>Fe-Mn binding fluorine>residual fluorine.The bacterial diversity analysis showed that the bacterial abundance reached the peak in the W-4 stage of pile-fermentation, and the dominant bacteria was Burkholderia-Caballeronia-Paraburkholderia at the genus level.The analysis of fungal diversity showed that Aspergillus was the dominant fungus at genus level during dark tea pile-fermentation, and its relative abundance ranged from 1.90% to 66.26% throughout the whole process of pile-fermentation.The correlation analysis between fluoride in different existing forms and microbial populations showed that the dominant genera Thermomyces and Blastobotrys had a strong positive correlation with the fluorine content of all forms at the middle stage of pile-fermentation, the dominant genera Wallemia and Rhizomucor showed a strong negative correlation with the fluorine content of all forms in the later stage of pile-fermentation, the bacterial community was related to the contents of Fe-Mn binding fluorine and exchangeable fluorine in the early stage of pile-fermentation, and the contents of total fluorine, organic bound fluorine, water-soluble fluorine and residual fluorine in the middle and late stage of pile-fermentation.In conclusion, it could be concluded that there was a significant correlation between the microbial community and the fluoride contents in different existing forms during the pile-fermentation process of dark tea.

Key words： dark tea; pile-fermentation; existing forms of fluorine; bacteria diversity; fungal diversity

Cite this article

GUO Mengyi , PAN Xueli , LUO Yan , LI Chengzhuo , HU Xianchun , ZHAO Zhenjun . Analysis on existing forms of fluorine and its correlation with microbial population during the pile-fermentation of dark tea[J]. Food and Fermentation Industries, 2025 , 51(17) : 195 -204 . DOI: 10.13995/j.cnki.11-1802/ts.041569

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