Postharvest fruit ascorbic acid (AsA) exists in both oxidized and reduced forms and the decline of AsA content during postharvest storage is a critical factor affecting fruit quality.To investigate the regulatory mechanism of 2,4-epibrassinolide (EBR) on AsA metabolism in postharvest strawberries, this study applied 5 μmol/L of EBR to strawberry fruit and conducted a 5-day storage experiment at (20±1) ℃ with 90%-95% relative humidity.Results showed that EBR treatment significantly inhibited the decline in reduced AsA, dehydroascorbic acid (DHA) and total AsA contents, while maintaining the AsA/DHA ratio.Transcriptome analysis revealed that EBR treatment regulated 3 309 differentially expressed genes, including 1 491 upregulated and 1 818 downregulated genes.Further analysis using quantitative real-time PCR (qRT-PCR) clarified the transcriptional changes in AsA metabolic pathway-related genes in EBR-treated strawberry fruits.Notably, the upregulated expression of FaMIOX and FaAPX genes positively contributed to AsA/DHA homeostasis, potentially regulated by WRKY family transcription factors.This study elucidates the molecular mechanism by which EBR delays AsA degradation in postharvest strawberries through the regulation of key gene expression, providing a theoretical basis and practical reference for the application of EBR in postharvest fruit preservation.
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