Sarcopenia is a senile syndrome characterized by the degeneration of muscle mass, strength and function, the occurrence and development of which are closely related to inflammation and oxidative stress.At present, the research on special dietary supplements to prevent or alleviate sarcopenia has just begin in China.In this study, three strains by antioxidant activity were firstly screened as indicator, including Lactiplantibacillus plantarum XC-2, L.plantarum NDF100S-2, L.plantarum HZZC-3.Based on the in vitro model of sarcopenia, the combined improvement effect of three strains of L.plantarum and β-hydroxy-β-methylbutyrate (HMB) with anti-inflammation activity on sarcopenia was investigated using the myotube diameter as indicator.Results showed that the both single strain and combination had the effect of improving insomnia, and the combination of L.plantarum NDF100S-2, L.plantarum HZZC-3, and HMB had the best effect, which significantly improved the surface roughness and stiffness of muscle tubes, and up-regulated the expression of fast muscle fibrin MYH1.This study provided a theoretical basis for improving sarcopenia by combining probiotics and HMB.
JIANG Lu
,
SUN Rongxin
,
XUE Liying
,
LIU Yisuo
,
CAO Jiayuan
,
YI Huaxi
. Study on improvement of sarcopenia by combination of Lactiplantibacillus plantarum and β-hydroxy-β-methylbutyrate[J]. Food and Fermentation Industries, 2025
, 51(18)
: 275
-285
.
DOI: 10.13995/j.cnki.11-1802/ts.041291
[1] GIELEN E, BECKWÉE D, DELAERE A, et al.Nutritional interventions to improve muscle mass, muscle strength, and physical performance in older people:An umbrella review of systematic reviews and meta-analyses[J].Nutrition Reviews, 2021, 79(2):121-147.
[2] WU X, LI X, XU M H, et al.Sarcopenia prevalence and associated factors among older Chinese population:Findings from the China Health and Retirement Longitudinal Study[J].PLoS One, 2021, 16(3):e0247617.
[3] 肌少症共识[J].中华骨质疏松和骨矿盐疾病杂志, 2016, 9(3):215-227.
Sarcopenia consensus[J].Chinese Journal of Osteoporosis and Bone Mineral Salt Diseases, 2016, 9(3):215-227.
[4] PICCA A, FANELLI F, CALVANI R, et al.Gut dysbiosis and muscle aging:Searching for novel targets against sarcopenia[J].Mediators of Inflammation, 2018, 2018(1):7026198.
[5] CHEN L K, ARAI H, ASSANTACHAI P, et al.Roles of nutrition in muscle health of community-dwelling older adults:Evidence-based expert consensus from Asian Working Group for Sarcopenia[J].Journal of Cachexia, Sarcopenia and Muscle, 2022, 13(3):1653-1672.
[6] YANG C, SONG Y, LI T, et al.Effects of beta-hydroxy-beta-methylbutyrate supplementation on older adults with sarcopenia:A randomized, double-blind, placebo-controlled study[J].The Journal of Nutrition, Health & Aging, 2023, 27(5):329-339.
[7] SANDERS M E.Probiotics and microbiota composition[J].BMC Medicine, 2016, 14(1):82.
[8] CHEN Y M, WEI L, CHIU Y S, et al.Lactobacillus plantarum TWK10 supplementation improves exercise performance and increases muscle mass in mice[J].Nutrients, 2016, 8(4):205.
[9] SHI Y J, CUI X Y, GU S S, et al.Antioxidative and probiotic activities of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China[J].Probiotics and Antimicrobial Proteins, 2019, 11(4):1086-1099.
[10] 杨婷玉. 青藏高原抗氧化特性乳酸菌筛选及其对肠道微生态的调节作用[D].兰州:兰州大学, 2023.
YANG T Y.Screening of lactic acid bacteria with antioxidant properties in Tibetan Plateau and its regulatory effect on intestinal microecology[D].Lanzhou:Lanzhou University, 2023.
[11] LIN M Y, CHANG F J.Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356[J].Digestive Diseases & Sciences, 2000, 45(8):1617-1622.
[12] GAO D W, GAO Z R, ZHU G H.Antioxidant effects of Lactobacillus plantarum via activation of transcription factor Nrf2[J].Food & Function, 2013, 4(6):982-989.
[13] KUT K, BARTOSZ G, SOSZYŃSKI M, et al. Antioxidant properties of hispidulin. Natural Product Research, 2022, 36(24):6401-6404.
[14] AHIRE J J, MOKASHE N U, PATIL H J, et al.Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6[J].Journal of Food Science & Technology, 2013, 50(1):26-34.
[15] WU H C, CHEN H M, SHIAU C Y.Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus)[J].Food Research International, 2003, 36(9-10):949-957.
[16] 王佳敏. 新疆传统奶酪中抗氧化乳酸菌优选及微胶囊化[D].石河子:石河子大学, 2022.
WANG J M.Optimization and microencapsulation of antioxidant lactic acid bacteria in Xinjiang traditional cheese[D].Shihezi:Shihezi University, 2022.
[17] MILARDOVIĆ S, IVEKOVIĆ D, GRABARIĆ B S.A novel amperometric method for antioxidant activity determination using DPPH free radical[J].Bioelectrochemistry, 2006, 68(2):175-180.
[18] 王惋, 侯俊财, 于彤, 等.具有抗氧化和抑菌能力的益生性乳酸菌筛选及鉴定[J].食品与发酵工业, 2020, 46(3):43-49.
WANG W, HOU J C, YU T, et al.Screening and identification of probiotic lactic acid bacteria strains with antioxidative and antimicrobial abilities[J].Food and Fermentation Industries, 2020, 46(3):43-49.
[19] 张开屏, 赵艳红, 李权威, 等.乳酸菌抗氧化特性及其katA基因分析[J].中国食品学报, 2021, 21(7):37-43.
ZHANG K P, ZHAO Y H, LI Q W, et al.Antioxidant properties of Lactobacillus and its katA gene analysis[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(7):37-43.
[20] 刘少敏. 不同乳酸菌抗氧化能力比较及其机制的研究[D].哈尔滨:东北农业大学, 2015.
LIU S M.Comparison of antioxidant capacity of different lactic acid bacteria and its mechanism[D].Harbin:Northeast Agricultural University, 2015.
[21] MAKRAS L, DE VUYST L.The in vitro inhibition of Gram-negative pathogenic bacteria by bifidobacteria is caused by the production of organic acids[J].International Dairy Journal, 2006, 16(9):1049-1057.
[22] 冯美琴, 栾晓旭, 孙健.3株发酵香肠源乳酸菌体外功能特性的比较[J].食品科学, 2020, 41(24):39-45.
FENG M Q, LUAN X X, SUN J.Comparative study on the in vitro functional characteristics of three strains of lactic acid bacteria isolated from fermented sausages[J].Food Science, 2020, 41(24):39-45.
[23] LIU C W, LIU A L, ZHOU J H, et al.Role and mechanism of theaflavins in regulating skeletal muscle inflammation[J].Journal of Agricultural and Food Chemistry, 2022, 70(41):13233-13250.
[24] WANG Y N, KEDLIAN V R.Systematic analysis of muscle aging using joint single-cell and single-nucleus sequencing[J].Nature Aging, 2024, 4(5):621-622.
