Food and Fermentation Industries >

2025 , Vol. 51 >Issue 18: 426 - 432

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042236

Research progress of bacteriophages as antimicrobial agents for food preservation in active packaging

  • DENG Jisi ,
  • SHI Hui
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  • (College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2025-01-23

  Revised date: 2025-03-07

  Online published: 2025-10-16

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Abstract

Bacteriophages are viruses with specific bactericidal properties, exhibiting targeted action against their host bacteria without harming beneficial flora.Commercialized bacteriophages have been approved by the U.S.Food and Drug Administration (FDA) as generally recognized as safe (GRAS) and can be used as antimicrobial agents in food packaging, specifically targeting various foodborne pathogens.Active packaging refers to packaging that contains active substances, which in turn safeguards the quality and safety of food during storage.The application of bacteriophages in active packaging can confer strong bacteriostatic properties to the packaging, while the packaging can improve phage stability and safeguard phage activity from food during the application process.This review introduces the concept of phages and active packaging, comprehensively discusses and summarizes the application of bacteriophages as bacteriostatic agents in food preservation packaging, including the types of composite materials, carriers and the methods of phage encapsulation and fixation.At the same time, the release mechanism of phage in active packaging is analyzed.This review provides theoretical support for effectively reducing the incidence of foodborne diseases, ensuring food safety and innovative applications of phages in food preservation in the future.

Key words： bacteriophage; active packaging; food; preservation; antibacterial agent

Cite this article

DENG Jisi , SHI Hui . Research progress of bacteriophages as antimicrobial agents for food preservation in active packaging[J]. Food and Fermentation Industries, 2025 , 51(18) : 426 -432 . DOI: 10.13995/j.cnki.11-1802/ts.042236

References

[1] VILELA C, KUREK M, HAYOUKA Z, et al.A concise guide to active agents for active food packaging[J].Trends in Food Science & Technology, 2018, 80:212-222.
[2] WANG J H, ZHENG Y Q, HUANG H K, et al.An overview of signal amplification strategies and construction methods on phage-based biosensors[J].Food Research International, 2024, 191:114727.
[3] JAHANDAR-LASHAKI S, FARAJNIA S, FARAJI-BARHAGH A, et al.Phage display as a medium for target therapy based drug discovery, review and update[J].Molecular Biotechnology, 2025,67(6):2161-2184.
[4] KIRCHHELLE C, LIPTON M F, NOLAN T M, et al.Reuse, repurpose, and recycle:Bacteriophages and microbial surveillance (1921-2023)[J].Phage, 2024, 5(1):14-21.
[5] OLUWARINDE B O, AJOSE D J, ABOLARINWA T O, et al.Safety properties of Escherichia coli O157:H7 specific bacteriophages:Recent advances for food safety[J].Foods, 2023, 12(21):3989.
[6] HUANG Z H, ZHANG Z H, TONG J R, et al.Phages and their lysins:Toolkits in the battle against foodborne pathogens in the postantibiotic era[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(4):3319-3343.
[7] HOU Y T, WU Z H, REN L, et al.Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila[J].Frontiers in Microbiology, 2023, 14:1178876.
[8] COSTA M J, PASTRANA L M, TEIXEIRA J A, et al.Bacteriophage delivery systems for food applications:Opportunities and perspectives[J].Viruses, 2023, 15(6):1271.
[9] VALENCIA-TOXQUI G, RAMSEY J.How to introduce a new bacteriophage on the block:A short guide to phage classification[J].Journal of Virology, 2024, 98(10):e0182123.
[10] ZHANG Y T, MIAO J J, ZHANG N, et al.The analysis of the function, diversity, and evolution of the Bacillus phage genome[J].BMC Microbiology, 2023, 23(1):170.
[11] NING Y, TENG T S, WU X H, et al.Development of an enzyme-linked phage receptor-binding protein assay (ELPRA) based on a novel biorecognition molecule- receptor-binding protein Gp130 of Pseudomonas aeruginosa bacteriophage Henu5[J].Enzyme and Microbial Technology, 2024, 177:110442.
[12] CHOO K W, MAO L, MUSTAPHA A.CAM-21, a novel lytic phage with high specificity towards Escherichia coli O157:H7 in food products[J].International Journal of Food Microbiology, 2023, 386:110026.
[13] LEE H J, KIM H J, LEE S J.Control of λ lysogenic Escherichia coli cells by synthetic λ phage carrying cIantisense[J].ACS Synthetic Biology, 2022, 11(11):3829-3835.
[14] ENGELBERG-KULKA H, KUMAR S.Yet another way that phage λ manipulates its Escherichia coli host:λ is involved in the lysogenic—lytic switch[J].Molecular Microbiology, 2015, 96(4):689-693.
[15] 陈学梅, 魏云林, 季秀玲.前噬菌体研究进展[J].遗传, 2021, 43(3):240-248.
CHEN X M, WEI Y L, JI X L.Research progress of prophages[J].Hereditas, 2021, 43(3):240-248.
[16] ZHANG B, WANG Y X, WANG F F, et al.Microencapsulated phage composites with increased gastrointestinal stability for the oral treatment of Salmonella colonization in chicken[J].Frontiers in Veterinary Science, 2023, 9:1101872.
[17] CUI H Y, YUAN L, LIN L.Novel chitosan film embedded with liposome-encapsulated phage for biocontrol of Escherichia coli O157:H7 in beef[J].Carbohydrate Polymers, 2017, 177:156-164.
[18] TYAGI P, SALEM K S, HUBBE M A, et al.Advances in barrier coatings and film technologies for achieving sustainable packaging of food products-a review[J].Trends in Food Science & Technology, 2021, 115:461-485.
[19] 孙彦梅, 吴世芳, 尚天翠, 等.壳聚糖-明胶-恰玛古提取物活性包装膜的制备及其对新疆褐牛肉保鲜效果的研究[J/OL].食品与发酵工业, 2025.https://doi.org/10.13995/j.cnki.11-1802/ts.041159.
SUN Y M, WU S F, SHANG T C, et al.Preparation of active packaging film of chitosan-gelatin-chamagu extract and its effect on fresh-keeping of Xinjiang brown beef[J/OL].Food and Fermentation Industries, 2025.https://doi.org/10.13995/j.cnki.11-1802/ts.041159.
[20] MOEINI A, GERMANN N, MALINCONICO M, et al.Formulation of secondary compounds as additives of biopolymer-based food packaging:A review[J].Trends in Food Science & Technology, 2021, 114:342-354.
[21] UPADHYAY A, AGBESI P, ARAFAT K M Y, et al.Bio-based smart packaging:Fundamentals and functions in sustainable food systems[J].Trends in Food Science & Technology, 2024, 145:104369.
[22] PERERA K Y, SHARMA S, PRADHAN D, et al.Seaweed polysaccharide in food contact materials (active packaging, intelligent packaging, edible films, and coatings)[J].Foods, 2021, 10(9):2088.
[23] WANG H X, HU L D, PENG L, et al.Dual encapsulation of β-carotene by β-cyclodextrin and chitosan for 3D printing application[J].Food Chemistry, 2022, 378:132088.
[24] MARIĆ M, GRASSINO A N, ZHU Z Z, et al.An overview of the traditional and innovative approaches for pectin extraction from plant food wastes and by-products:Ultrasound-, microwaves-, and enzyme-assisted extraction[J].Trends in Food Science & Technology, 2018, 76:28-37.
[25] RICHARDS K, MALIK D J.Microencapsulation of bacteriophages using membrane emulsification in different pH-triggered controlled release formulations for oral administration[J].Pharmaceuticals, 2021, 14(5):424.
[26] WENG S H, LÓPEZ A, SÁEZ-ORVIZ S, et al.Effectiveness of bacteriophages incorporated in gelatine films against Staphylococcus aureus[J].Food Control, 2021, 121:107666.
[27] VONASEK E L, CHOI A H, SANCHEZ J Jr, et al.Incorporating phage therapy into WPI dip coatings for applications on fresh whole and cut fruit and vegetable surfaces[J].Journal of Food Science, 2018, 83(7):1871-1879.
[28] AMARILLAS L, LIGHTBOURN-ROJAS L, ANGULO-GAXIOLA A K, et al.The antibacterial effect of chitosan-based edible coating incorporated with a lytic bacteriophage against Escherichia coli O157:H7 on the surface of tomatoes[J].Journal of Food Safety, 2018, 38(6):e12571.
[29] PARK J, HASSAN M A, NABAWY A, et al.Engineered bacteriophage-polymer nanoassemblies for treatment of wound biofilm infections[J].ACS Nano, 2024, 18(39):26928-26936.
[30] PACIOS-MICHELENA S, RODRÍGUEZ-HERRERA R, RINCÓN-ENRÍQUEZ G, et al.Effect of encapsulation and natural polyphenolic compounds on bacteriophage stability and activity on Escherichia coli in Lactuca sativa L.var.longifolia[J].Journal of Food Safety, 2023, 43(2):e13000.
[31] CHOI I, LEE J S, HAN J.Maltodextrin-trehalose miscible system-based bacteriophage encapsulation:Studies of plasticizing effect on encapsulated phage activity and food application as an antimicrobial agent[J].Food Control, 2023, 146:109550.
[32] CUI H Y, YANG X J, LI C Z, et al.Enhancing anti-E.coli O157:H7 activity of composite phage nanofiber film by D-phenylalanine for food packaging[J].International Journal of Food Microbiology, 2022, 376:109762.
[33] LORENZO-REBENAQUE L, MALIK D J, CATALÁ-GREGORI P, et al.Microencapsulated bacteriophages incorporated in feed for Salmonella control in broilers[J].Veterinary Microbiology, 2022, 274:109579.
[34] PETSONG K, BENJAKUL S, VONGKAMJAN K.Optimization of wall material for phage encapsulation via freeze-drying and antimicrobial efficacy of microencapsulated phage against Salmonella[J].Journal of Food Science and Technology, 2021, 58(5):1937-1946.
[35] KOO C K W, SENECAL K, SENECAL A, et al.Dehydration of bacteriophages in electrospun nanofibers:Effect of excipients in polymeric solutions[J].Nanotechnology, 2016, 27(48):485102.
[36] JU T, LI J X, WESTON A, et al.Anti-Pseudomonas aeruginosa bacteriophage loaded electrospun fibers for antibacterial wound dressings[J].Macromolecular Rapid Communications, 2025,46(13).
[37] RIOS A C, VILA M M D C, LIMA R, et al.Structural and functional stabilization of bacteriophage particles within the aqueous core of a W/O/W multiple emulsion:A potential biotherapeutic system for the inhalational treatment of bacterial pneumonia[J].Process Biochemistry, 2018, 64:177-192.
[38] ALVES D, CERQUEIRA M A, PASTRANA L M, et al.Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella enteritidis[J].Food Research International, 2020, 128:108791.
[39] ELSAYED A, SAFWAT A, ABDELSATTAR A S, et al.The antibacterial and biofilm inhibition activity of encapsulated silver nanoparticles in emulsions and its synergistic effect with E.coli bacteriophage[J].Inorganic and Nano-Metal Chemistry, 2023, 53(6):549-559.
[40] LIN L, ZHU Y L, CUI H Y.Inactivation of Escherichia coli O157:H7 treated by poly-L-lysine-coated bacteriophages liposomes in pork[J].Journal of Food Safety, 2018, 38(6):e12535.
[41] SINGLA S, HARJAI K, RAZA K, et al.Phospholipid vesicles encapsulated bacteriophage:A novel approach to enhance phage biodistribution[J].Journal of Virological Methods, 2016, 236:68-76.
[42] KIM S, KIM B S, BAI J, et al.Antibacterial κ-carrageenan/konjac glucomannan-based edible hydrogel film containing Salmonella phage PBSE191 and its application in chicken meat[J].LWT, 2023, 180:114707.
[43] GONG C X, GUAN W, LIU X M, et al.Biomimetic bacteriophage-like particles formed from probiotic extracts and NO donors for eradicating multidrug-resistant staphylococcus aureus[J].Advanced Materials, 2022, 34(45):e2206134.
[44] KHANAL D, CHANG R Y K, HICK C, et al.Enteric-coated bacteriophage tablets for oral administration against gastrointestinal infections[J].International Journal of Pharmaceutics, 2021, 609:121206.
[45] VINNER G K, REZAIE-YAZDI Z, LEPPANEN M, et al.Microencapsulation of Salmonella-specific bacteriophage felix O1 using spray-drying in a pH-responsive formulation and direct compression tableting of powders into a solid oral dosage form[J].Pharmaceuticals, 2019, 12(1):43.
[46] YANG Y F, DU H, ZOU G, et al.Encapsulation and delivery of phage as a novel method for gut flora manipulation in situ:A review[J].Journal of Controlled Release, 2023, 353:634-649.
[47] MEYER A, GREENE M, KIMMELSHUE C, et al.Stabilization of T4 bacteriophage at acidic and basic pH by adsorption on paper[J].Colloids and Surfaces, B:Biointerfaces, 2017, 160:169-176.
[48] FRANCIUS G, CERVULLE M, CLMENT E, et al.Impacts of mechanical stiffness of bacteriophage-loaded hydrogels on their antibacterial activity[J].ACS Applied Bio Materials, 2021, 4(3):2614-2627.
[49] PEARSON H A, SAHUKHAL G S, ELASRI M O, et al.Phage-bacterium war on polymeric surfaces:Can surface-anchored bacteriophages eliminate microbial infections?[J].Biomacromolecules, 2013, 14(5):1257-1261.
[50] ZHAO Y, LI B, ZHANG W P, et al.Recent advances in sustainable antimicrobial food packaging:Insights into release mechanisms, design strategies, and applications in the food industry[J].Journal of Agricultural and Food Chemistry, 2023, 71(31):11806-11833.
[51] MALEKJANI N, KARIMI R, ASSADPOUR E, et al.Control of release in active packaging/coating for food products;approaches, mechanisms, profiles, and modeling[J].Critical Reviews in Food Science and Nutrition, 2024, 64(29):10789-10811.
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