Food and Fermentation Industries >

2025 , Vol. 51 >Issue 20: 93 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041772

Protective effect of cold stress on activity of freeze-dried Lacticaseibacillus rhamnosus B6

  • YANG Ting ,
  • ZHANG Jia ,
  • ZHANG Juan ,
  • WU Zhengjun
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  • 1(State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co.Ltd., Shanghai 200436, China)
    2(College of Life Science, Shanghai University, Shanghai 200444, China)

Received date: 2024-12-04

  Revised date: 2025-03-10

  Online published: 2025-10-27

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Abstract

To explore the impact of cold stress on the activity of freeze-dried lactic acid bacteria, Lacticaseibacillus rhamnosus B6 was selected as the research subject.Seven protectants and various cold stress treatments were evaluated for their protective effects during freeze-drying.Additionally, this study explored the patterns of changes in survival rate, acidification capacity, lactate dehydrogenase activity, and cell membrane integrity of strain B6 under various cold stress temperature and durations.Results indicated that 100 g/L skim milk was the optimal protectant, achieving a high freeze survival rate of 79.30%.This survival rate was significantly higher than that of other protectants (P<0.05).With 100 g/L skimmed milk as protective agent, the survival rate of freeze-drying could reach (97.94±1.1)% under cold stress at 4 ℃ for 2 h.Compared with the group without cold stress treatment, the survival rate of cold stress at 4 ℃ for 2 h increased by 18.64%.Moreover, the effect of this cold stress condition was significantly better than other cold stress conditions (P<0.05).Furthermore, results showed that cold stress at 4 ℃ for 2 h could significantly improve the acidification ability and lactate dehydrogenase activity of L.rhamnosus B6 after freeze-drying, and maintain the integrity of cell membrane.Therefore, this study demonstrated that cold stress treatment was an effective method for preserving the viability of freeze-dried L.rhamnosus.

Key words： cold stress; freeze-drying; Lacticaseibacillus rhamnosus; survival rate; acidification

Cite this article

YANG Ting , ZHANG Jia , ZHANG Juan , WU Zhengjun . Protective effect of cold stress on activity of freeze-dried Lacticaseibacillus rhamnosus B6[J]. Food and Fermentation Industries, 2025 , 51(20) : 93 -100 . DOI: 10.13995/j.cnki.11-1802/ts.041772

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