Food and Fermentation Industries >

2025 , Vol. 51 >Issue 20: 122 - 130

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041716

Network pharmacology study on inhibition of pathogenic Escherichia coli by active ingredients of Glycyrrhiza uralensis

  • YANG Bingqian ,
  • YUN Chenke ,
  • LIU Lei ,
  • CHEN Tianyi ,
  • LI Bingfeng ,
  • ZHANG Sen ,
  • CHANG Siyuan
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  • 1(Bio-based Platform Chemicals Catalysis Engineering Technology Research and Development Center of Jiangsu Province, Nanjing Polytechnic Institute, Nanjing 210048, China)
    2(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China)

Received date: 2024-11-27

  Revised date: 2025-03-07

  Online published: 2025-10-27

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Abstract

This study used network pharmacology and molecular docking technology to explore the inhibitory mechanism of active ingredients in Glycyrrhiza uralensis on pathogenic Escherichia coli and verified by in vitro bacteriostatic test.The active ingredients and targets of G.uralensis were screened in TCMSP databases.Genecards and OMIM databases were searched for targets that inhibit E.coli.The jvenn platform was used to obtain intersection targets.The protein-protein interaction (PPI) analysis of the intersection targets was performed using the STRING platform, followed by GO function and KEGG pathway enrichment analyses using the Metascape platform.After obtaining the key targets inhibiting 5 pathogenic E.coli, the effect of primary active ingredients was identified by molecular docking and in vitro bacteriostatic test.After screening, 86 active ingredients and 93 intersection targets were obtained.Through GO and KEGG enrichment analysis, G.uralensis mainly affected biological processes such as response to xenobiotic stimulus and response to molecule of bacterial origin, and pathways like pathways in cancer, lipid and atherosclerosis pathways.Key targets for inhibiting 5 pathogenic E.coli were IL6, TP53, and STAT3, and molecular docking showed that the naringenin, isoformononetin, and 3″-methoxyglabridin exhibited high binding energy to these targets.The minimal inhibit concentration of above 3 active ingredients against 5 pathogenic E.coli varied between 0.16-0.31, 0.62-1.25, and 0.07-0.16 μg/mL, respectively, and minimum bactericidal concentration varied between 0.16-0.62, 1.25-2.50, and 0.16-0.31 μg/mL, respectively.3″-Methoxyglabridin displayed the best antibacterial activity.This study provides the basis for exploring the mechanism of G.uralensis in inhibiting E.coli and key active ingredients in G.uralensis for inhibiting pathogenic E.coli.

Key words： network pharmacology; Glycyrrhiza uralensis; pathogenic Escherichia coli; molecular docking; in vitro bacteriostatic test

Cite this article

YANG Bingqian , YUN Chenke , LIU Lei , CHEN Tianyi , LI Bingfeng , ZHANG Sen , CHANG Siyuan . Network pharmacology study on inhibition of pathogenic Escherichia coli by active ingredients of Glycyrrhiza uralensis[J]. Food and Fermentation Industries, 2025 , 51(20) : 122 -130 . DOI: 10.13995/j.cnki.11-1802/ts.041716

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