Food and Fermentation Industries >

2025 , Vol. 51 >Issue 19: 238 - 247

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042967

Construction, structural characterization and digestion characteristics of oyster peptides delivery system based on nano-calcium carbonate

  • WANG Jing ,
  • ZHANG Mengmeng ,
  • ZHANG Zhuoqian ,
  • CAO Wenhong ,
  • TAN Mingtang ,
  • ZHU Guoping ,
  • GAO Jialong ,
  • LIN Haisheng ,
  • ZHENG Huina ,
  • CHEN Zhongqin
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China)
    2(Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China)

Received date: 2025-04-09

  Revised date: 2025-05-30

  Online published: 2025-11-03

Fold

Abstract

Oyster bioactive peptides (OBP) exhibit multiple biological activities including antioxidant, hypoglycemic, and hypolipidemic effects.However, the poor stability of OBP in gastrointestinal environments significantly limits their efficacy.To enhance the stability of OBP and reduce the waste of oyster shell resources, this study first synthesized nano-calcium carbonate (CaCO3 NPs) from oyster shell.The CaCO3 NPs were then modified with sodium alginate (ALG), and the modification process was optimized.Subsequently, OBP was loaded onto ALG-CaCO3 NPs, followed by polyethylene glycol modification, to construct an OBP-CaCO3 NPs nano-system.Finally, the study investigated the structural stability, in vitro release, and digestive characteristics of the nano-system.The orthogonal experimental results indicated that the optimal modification process parameters for CaCO3 NPs were:ALG concentration of 5 mg/mL, temperature of 35 ℃, and reaction time of 2 h.The nano-system showed an average particle size of (288.7±7.6) nm and zeta potential of (-30.5±0.8) mV, achieving encapsulation efficiency of (35.69±2.22)% and loading capacity of (19.71±1.60)%.Fourier transform infrared spectroscopy analysis revealed that ALG modified CaCO3 NPs by forming an ‘egg box’ structure through the cross-linking of carboxyl group and Ca2+, while OBP were loaded inside the carrier through electrostatic effect.The results of in vitro release experiments showed that the cumulative release rate of the nano-system in the simulated gastric environment (pH 2.0) for 36 hours was only (40.31±0.63)%, while the release rate in the intestinal environment (pH 6.8) significantly increased to (93.12±3.76)%, indicating the nano-system had the characteristics of targeted intestinal slow release.Digestion stability experiments demonstrated that the nano-system could significantly improve the pancreatic lipase inhibitory activity of OBP, the inhibitory rate increased from (23.66±0.93)% to (54.73±1.37)% after gastric digestion, and from (4.75±0.38)% to (24.67±1.31)% after gastrointestinal digestion.This study provides theoretical support for the design of stable delivery systems for functional peptides, while offering a novel approach for the high-value utilization of oyster shell waste.

Key words： oyster peptides; nano-calcium carbonate; sodium alginate modification; nano delivery system; digestive stability

Cite this article

WANG Jing , ZHANG Mengmeng , ZHANG Zhuoqian , CAO Wenhong , TAN Mingtang , ZHU Guoping , GAO Jialong , LIN Haisheng , ZHENG Huina , CHEN Zhongqin . Construction, structural characterization and digestion characteristics of oyster peptides delivery system based on nano-calcium carbonate[J]. Food and Fermentation Industries, 2025 , 51(19) : 238 -247 . DOI: 10.13995/j.cnki.11-1802/ts.042967

References

[1] 赵强, 魏祥玲, 孙建安, 等.牡蛎资源的综合开发利用研究进展[J].中国食品添加剂, 2021, 32(7):150-159.
ZHAO Q, WEI X L, SUN J A, et al.Research progress on comprehensive utilization of oyster resources[J].China Food Additives, 2021, 32(7):150-159.
[2] 曹文红, 章超桦.生物活性肽的吸收机制[J].药物生物技术, 2006, 13(5):384-388.
CAO W H, ZHANG C H.Absorption mechanism of peptides in the gastrointestinal tract[J].Pharmaceutical Biotechnology, 2006, 13(5):384-388.
[3] 韦承忠. 牡蛎壳在化工生产和环境保护资源化利用中的研究进展[J].食品工业, 2022, 43(10):202-207.
WEI C Z.Research progress of oyster shell resource utilization in chemical production and environmental protection[J].The Food Industry, 2022, 43(10):202-207.
[4] JADHAV V, ROY A, KAUR K, et al.Recent advances in nanomaterial-based drug delivery systems[J].Nano-Structures & Nano-Objects, 2024, 37:101103.
[5] VERGARO V, PAPADIA P, LEPORATTI S, et al.Synthesis of biocompatible polymeric nano-capsules based on calcium carbonate:A potential cisplatin delivery system[J].Journal of Inorganic Biochemistry, 2015, 153:284-292.
[6] RAO C H, LI M, SUN X Q, et al.Preparation and characterization of phosphate-stabilized amorphous calcium carbonate nanoparticles and their application in curcumin delivery[J].Materials Chemistry and Physics, 2020, 255:123552.
[7] 张佩. CMCS/CMC复合水凝胶负载牡蛎降糖肽稳态体系的构建及其吸收转运机制研究[D].湛江:广东海洋大学, 2023.
ZHANG P.Construction of a stable system of oyster hypoglycemic peptides based on CMCS/CMC composite hydrogel and its absorption and transport mechanism[D].Zhanjiang:Guangdong Ocean University, 2023.
[8] 崔巍. 负载BMP-2小分子活性肽的介孔二氧化硅纳米微球结合煅烧骨支架材料诱导骨再生的实验研究[D].武汉:华中科技大学, 2017.
CUI W.Induction of bone regeneration:a novel combination of mesoporous silica nanoparticles loaded with BMP-2-related peptide combined with true bone ceramics scaffold[D].Wuhan:Huazhong University of Science & Technology, 2017.
[9] RAJCHAKIT U, LAMBA S, WANG K, et al.Size-controlled synthesis of gold nanoparticles tethering antimicrobial peptides with potent broad-spectrum antimicrobial and antibiofilm activities[J].Molecular Pharmaceutics, 2024, 21(2):596-608.
[10] GAUTAM M, SANTHIYA D, DEY N.Zein coated calcium carbonate nanoparticles for the targeted controlled release of model antibiotic and nutrient across the intestine[J].Materials Today Communications, 2020, 25:101394.
[11] 苏小洁, 陈忠琴, 曹文红, 等.牡蛎DPP-Ⅳ抑制肽的结构特征及其协同花色苷的活性增效作用研究[J].食品与发酵工业, 2024, 50(15):87-96.
SU X J, CHEN Z Q, CAO W H, et al.Structural characterization and synergistic inhibition of DPP-Ⅳ activity of oyster peptides and anthocyanins[J].Food and Fermentation Industries, 2024, 50(15):87-96.
[12] CHEN H, DONG X T, ZHAO Y Q, et al.Oyster-derived peptides as pancreatic lipase inhibitors:Kinetics, molecular interactions, and gastrointestinal stability[J].Food Bioscience, 2025, 63:105637.
[13] 赵历, 卓民权, 龚福忠, 等.碳化法制备球霰石碳酸钙微球及形成机理[J].无机盐工业, 2021, 53(3):38-43.
ZHAO L, ZHUO M Q, GONG F Z, et al.Synthesis of vaterite CaCO3 microspheres by carbonization method and its formation mechanism[J].Inorganic Chemicals Industry, 2021, 53(3):38-43.
[14] 徐梦晴. 纳米碳酸钙载药体系构建及其用于肿瘤精准治疗研究[D].武汉:华中农业大学, 2021.
XU M Q.Construction of nano calcium carbonate drug delivery system for tumor precision therapy[D].Wuhan:Huazhong Agricultural University, 2021.
[15] POLITI F A S, CARVALHO S G, RODERO C F, et al.Piperine-loaded nanoparticles incorporated into hyaluronic acid/sodium alginate-based membranes for the treatment of inflammatory skin diseases[J].International Journal of Biological Macromolecules, 2023, 227:736-748.
[16] 徐薇, 李慧雪, 刘霄莹, 等.海藻酸盐纳米载体递送亲脂性活性物质及其应用研究进展[J].食品科学, 2024, 45(17):242-252.
XU W, LI H X, LIU X Y, et al.Research progress on alginate nanocarrier delivery of lipophilic active substances and its application[J].Food Science, 2024, 45(17):242-252.
[17] 李准, 李旋坤, 孙雯, 等.海藻酸钠修饰对纳米SiO2在水中团聚行为的影响及机制[J].山东化工, 2020, 49(12):39-41.
LI Z, LI X K, SUN W, et al.Effect and mechanismof sodium alginate modification on the aggregation of nano-SiO2 in water[J].Shandong Chemical Industry, 2020, 49(12):39-41.
[18] LIU Y J, YU B R, DAI X G, et al.Biomineralized calcium carbonate nanohybrids for mild photothermal heating-enhanced gene therapy[J].Biomaterials, 2021, 274:120885.
[19] 谷峙樾. 协同转运复合纳米球的制备与性能[D].唐山:华北理工大学, 2019.
GU Z Y.Preparation and properties of cooperative transport composite nanospheres[D].Tangshan:North China University of Science and Technology, 2019.
[20] LEI W Z, QI M D, SONG J L, et al.Adsorption of polyphenolic compounds by sodium alginate-modified starch nanoparticles in a multiphase system:Kinetic, thermodynamic and release characteristics studies[J].Food Hydrocolloids, 2025, 162:110900.
[21] 赵应许, 纵瑞强, 高欣玉, 等.XDLVO理论解析不同离子条件下海藻酸钠微滤膜污染[J].环境科学, 2014, 35(4):1343-1350.
ZHAO Y X, ZONG R Q, GAO X Y, et al.Fouling behavior of sodium alginate during microfiltration at various ionic compositions:XDLVO approach[J].Environmental Science, 2014, 35(4):1343-1350.
[22] 庞子健, 徐源, 姜硕, 等.温度响应型淀粉醚/海藻酸钠复合凝胶的制备及其性能[J].大连海洋大学学报, 2022, 37(3):505-512.
PANG Z J, XU Y, JIANG S, et al.Preparation and characterization of thermoresponsive starch ether/sodium alginate composite hydrogels[J].Journal of Dalian Ocean University, 2022, 37(3):505-512.
[23] ZHANG X J, WANG K, HU J Y, et al.Role of a high calcium ion content in extending the properties of alginate dual-crosslinked hydrogels[J].Journal of Materials Chemistry A, 2020, 8(47):25390-25401.
[24] CAO N P, ZHAO Y H, CHEN H B, et al.Poly(ethylene glycol) becomes a supra-polyelectrolyte by capturing hydronium ions in water[J].Macromolecules, 2022, 55(11):4656-4664.
[25] XU M Z, QI Y M, LIU G S, et al.Size-dependent in vivo transport of nanoparticles:Implications for delivery, targeting, and clearance[J].ACS Nano, 2023, 17(21):20825-20849.
[26] 赵健辉. 甘草次酸修饰碳酸钙空腔纳米球的肝靶向研究[D].唐山:华北理工大学, 2020.
ZHAO J H.Liver targeting of glycyrrhetinic acid modified calcium carbonate hollow nanospheres[D].Tangshan:North China University of Science and Technology, 2020.
[27] 宋晓慧, 潘明宇, 徐坚峰, 等.球霰石在药物装载及控释中的应用[J].生物化学与生物物理进展, 2025, 52(1):162-181.
SONG X H, PAN M Y, XU J F, et al.Applications of vaterite in drug loading and controlled release[J].Progress in Biochemistry and Biophysics, 2025, 52(1):162-181.
[28] 窦佳红. 瞬时纳米沉淀法制备球霰石型碳酸钙及其负载抗癌药物缓控释的研究[D].上海:华东理工大学, 2020.
DOU J H.Preparation of vaterite CaCO3 particles by flash nano-precipitation technique for targeted and extended anticancer drug delivery[D].Shanghai:East China University of Science and Technology, 2020.
[29] 池如镜, 王志超, 张梦博, 等.海藻酸钠修饰磁性纳米粒子驱动液及其正渗透分离性能[J].膜科学与技术, 2020, 40(4):72-79.
CHI R J, WANG Z C, ZHANG M B, et al.Sodium alginate modified magnetic nanoparticle draw solution and its forward osmosis performance[J].Membrane Science and Technology, 2020, 40(4):72-79.
[30] DONG Y J, HE Y H, FAN D D, et al.Preparation of pH-sensitive chitosan-deoxycholic acid-sodium alginate nanoparticles loaded with ginsenoside Rb1 and its controlled release mechanism[J].International Journal of Biological Macromolecules, 2023, 234:123736.
[31] 回瑞华, 关崇新, 侯冬岩.羧酸及其盐红外光谱特性的研究[J].鞍山师范学院学报, 2001, 3(1):95-98.
HUI R H, GUAN C X, HOU D Y.Study on IR characteristics of carboxylic acid and their salts[J].Journal of Anshan Teachers College, 2001, 3(1):95-98.
[32] 牛冠超. pH响应型海藻酸钠基水凝胶对染料吸附性能研究[D].哈尔滨:黑龙江大学, 2024.
NIU G C.Study on the adsorption properties of pH responsive sodium alginate based hydrogels for dyes[D].Harbin:Heilongjiang University, 2024.
[33] MICHELY L, CHESNEAU C, DIKA E, et al.Easy way for fabricating calcium carbonate hybrid microparticles-supported carrier:Focus on the loading of several hydrosoluble cargos all at once[J].Journal of Drug Delivery Science and Technology, 2022, 74:103485.
[34] 刘园园. 以磷酸钙为基材构建负载阿贝西利和维生素E琥珀酸酯双药共递体系的研究[D].成都:四川大学, 2021.
LIU Y Y, Study on the construction of a dual-drug co-delivery system loaded with abemaciclib and vitamin E succinate based on calcium phosphate[D].Chengdu:` Sichuan University, 2021.
[35] 高就, 吴俊杰, 焦文雅, 等.6-姜烯酚玉米醇溶蛋白纳米颗粒的构建及其生物利用度分析[J].食品科学, 2024, 45(9):44-50.
GAO J, WU J J, JIAO W Y, et al.Construction and bioavailability analysis of 6-shogaol-loaded zein nanoparticles[J].Food Science, 2024, 45(9):44-50.
[36] 王维聪, 张馨睿, 滕敏, 等.pH敏感型海藻酸钠/木炭缓释凝胶球的制备及性能[J].林业工程学报, 2022, 7(2):128-134.
WANG W C, ZHANG X R, TENG M, et al.Preparation and performance of pH sensitive sodium alginate/charcoal slow-release gel spheres[J].Journal of Forestry Engineering, 2022, 7(2):128-134.
[37] 王华, 苏小洁, 陈忠琴, 等.不同处理方式对牡蛎降糖肽结构序列及消化特性的影响[J].食品工业科技, 2025,46(13):55-64.
WANG H, SU X J, CHEN Z Q, et al.Effect of different treatments on the structure sequence and digestive characteristics of oyster hypoglycemic peptides[J].Science and Technology of Food Industry, 2025,46(13):55-64.
[38] ZHU D Y, YUAN Z, WU D, et al.The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates[J].Food Science and Human Wellness, 2023, 12(5):1609-1617.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.