Food and Fermentation Industries >

2025 , Vol. 51 >Issue 21: 176 - 181

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042253

Biogenic amine formation in mixed lactic acid bacteria fermentation and the effect of adding American ginseng extract

  • LUO Yuqiao ,
  • WU Zhengyun ,
  • HUANG Huajian ,
  • ZHANG Wenxue
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  • (College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China)

Received date: 2025-01-24

  Revised date: 2025-03-26

  Online published: 2025-11-21

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Abstract

To explore biogenic amine (BA) formation in mixed lactic acid bacteria (LAB) fermentation, Lactobacillus brevis A1-8 (BA-producing LAB I) and L.brevis B2 (BA-producing LAB II) were mixed with Lactiplantibacillus plantarum-6 (non-BA-producing LAB) to form mixed LAB system I and II, respectively, and the possibility of adding American ginseng extract to control BA level of mixed LAB system was discussed.The results showed that, for mixed LAB system I, there was no significant difference in BA level among the groups with different proportions of BA-producing and non-BA-producing LAB;For mixed LAB system II, BA content decreased with the increase of inoculation ratio of non-BA-producing LAB, mainly due to the slow growth rate and weak competitiveness of BA-producing LAB II.With the addition of American ginseng extract, the pH value and the total acid contents of the two mixed LAB fermentation systems, the total number of LAB and the number of BA-producing LAB in the mixed LAB system I were not affected significantly;While the total number of LAB and the number of BA-producing LAB in mixed LAB system II decreased slightly;The BA level in mixed LBA systems I and II were reduced by 10%-20% and 20%-30%, respectively.Enzyme reaction tests revealed that the activity of tyrosine decarboxylase and ornithine decarboxylase decreased by 27% and 58%, respectively, by adding American ginseng extract.This study could provide a reference for the control of BA in fermentation processes dominated by LAB.

Key words： lactic acid bacteria; mixed bacteria fermentation; biogenic amine; American ginseng; amino acid decarboxylase

Cite this article

LUO Yuqiao , WU Zhengyun , HUANG Huajian , ZHANG Wenxue . Biogenic amine formation in mixed lactic acid bacteria fermentation and the effect of adding American ginseng extract[J]. Food and Fermentation Industries, 2025 , 51(21) : 176 -181 . DOI: 10.13995/j.cnki.11-1802/ts.042253

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