Impact of dietary sialoglycopeptides glycosylation on pathogenic microorganisms and gut microbiota in elderly individuals

  • ZHU Jiao ,
  • WU Jianrong ,
  • NIU Wenxuan ,
  • FAN Qunyan ,
  • LIU Xuncai
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  • 1(School of Biotechnology, Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China)
    2(Bird’s Nest Institute, Xiamen Yan Palace Seelong Food Co.Ltd., Xiamen 361000, China)

Received date: 2025-01-08

  Revised date: 2025-04-04

  Online published: 2025-12-15

Abstract

Protein glycosylation modulates the structural properties and biological functions of glycoproteins.Edible bird’s nest (EBN) and casein glycomacropeptide (CGMP) are both highly sialylated glycoprotein and glycopeptide.EBN is characterized by the presence of both N-glycan and O-glycan modifications, whereas CGMP contains exclusively O-glycans.This study compared the biological functions of EBN glycopeptides and CGMP, including their inhibitory effects on pathogens such as Candida albicans, Helicobacter pylori, and avian influenza virus (AIV), as well as their modulatory effects on the structure of the intestinal flora in elderly individuals.The controls used in this study include desialylated EBN glycopeptides, N-acetylneuraminic acid, and 3′-sialyllactose.DNA sequencer-based fluorescent glycan electrophoretic analysis revealed that EBN contained multiantennary N-glycans with sialylation and bisecting N-acetylglucosamine glycan chains.Results indicated that EBN glycopeptides could significantly inhibit C.albicans and H.pylori compared to CGMP.This inhibitory activity was substantially diminished when the terminal sialic acid residues of the EBN glycopeptides were removed (P<0.05).It was also found that EBN glycopeptides could effectively inhibit the adhesion of AIV to MDCK cells.In vitro fecal fermentation using fecal samples from elderly individuals demonstrated that EBN glycopeptides exhibited remarkable probiotic effects on the gut microbiota.In conclusion, the sialylation structure of glycoproteins played a crucial role in determining their biological activities.EBN glycopeptides, which were rich in both sialylated N-glycans and O-glycans, demonstrated a robust ability to inhibit pathogenic microorganisms and exhibited superior probiotic properties compared to CGMP that contained only O-glycosylation.

Cite this article

ZHU Jiao , WU Jianrong , NIU Wenxuan , FAN Qunyan , LIU Xuncai . Impact of dietary sialoglycopeptides glycosylation on pathogenic microorganisms and gut microbiota in elderly individuals[J]. Food and Fermentation Industries, 2025 , 51(22) : 89 -100 . DOI: 10.13995/j.cnki.11-1802/ts.042076

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