1,6-Hexanedial serves as a key precursor for polyamides(such as nylon-6,6), polyesters, and polyurethanes.However, its chemical synthesis suffers from high costs, poor sustainability, and environmental risks.To develop an eco-friendly biosynthetic route, this study constructed an 1,6-hexanedial production pathway using adipic acid as the substrate, and enhanced biosynthesis efficiency through targeted gene knockout and fermentation optimization, combined with in vitro catalysis to alleviate product toxicity, thereby improving 1,6-hexanedial accumulation.Results demonstrate that knockout of specific endogenous aldehyde reductase genes combined with fermentation optimization markedly increased yields.The engineered strain achieved 190 mg/L 1,6-hexanedial via in vivo fermentation, representing a 20.3-fold improvement over the parental strain.Subsequent in vitro catalysis elevated production to 295.8 mg/L, corresponding to a 32.2-fold enhancement.This titer stands as the highest reported value in 1,6-hexanedial biosynthesis to date.This work establishes a foundational strategy for biosynthesizing aliphatic dialdehydes.
CHENG Nan
,
ZHANG Quan
,
LI Guohui
,
MAO Yin
,
DENG Yu
. Research on the biosynthesis of 1,6-hexanedial[J]. Food and Fermentation Industries, 2025
, 51(23)
: 30
-36
.
DOI: 10.13995/j.cnki.11-1802/ts.042399
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