Food and Fermentation Industries >

2025 , Vol. 51 >Issue 24: 129 - 136

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042895

Optimization of sea buckthorn seed oil extraction and analysis of its fatty acid and triacylglycerol composition

  • TIAN Yu ,
  • REN Guangling ,
  • WANG Shuya ,
  • CAO Xi
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  • 1(College of Food and Bioengineering, Xihua University, Chengdu 610039, China)
    2(College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China)

Received date: 2025-03-31

  Revised date: 2025-05-01

  Online published: 2026-01-12

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Abstract

To promote the development and utilization of sea buckthorn seed oil, this study was carried out to optimize the extraction conditions using response surface methodology.Furthermore, the fatty acid composition and positional distribution were analyzed using GC, and the triacylglycerol composition and the major triacylglycerol type of the extracted sea buckthorn seed oil were analyzed using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Results showed that the optimal extraction conditions were crushing time of 12 s, solvent petroleum ether, sea buckthorn seed powder quality of 1.8 g (material-liquid ratio of 1∶33.3), and soaking time of 31 min.Under these conditions, the oil content of the sea buckthorn seeds was found to be 9.36%.A total of 14 fatty acids were detected in sea buckthorn seed oil.The most abundant were α-linolenic acid (30.37%), linoleic acid (30.17%), and oleic acid (20.21%).These three fatty acids also had the highest contents in the sn-2 position of the oil.All triacylglycerols in sea buckthorn seed oil were unsaturated fatty acids in at least one position, and 68.52% of the triacylglycerols were unsaturated fatty acids in all three positions.The most abundant triacylglycerols were OLL (34.15%) and OOL (26.33%), where O represented oleic acid and L represents linoleic acid.In this study, the isomeric configurations of 10 triacylglycerols containing more than 1% were also analyzed and determined based on the abundance ratio of MS/MS ions.This study provides a foundation for further research and utilization of sea buckthorn seed oil.

Key words： sea buckthorn seed oil; extraction; fatty acid composition; fatty acid positional distribution; triacylglycerol composition; triacylglycerol type

Cite this article

TIAN Yu , REN Guangling , WANG Shuya , CAO Xi . Optimization of sea buckthorn seed oil extraction and analysis of its fatty acid and triacylglycerol composition[J]. Food and Fermentation Industries, 2025 , 51(24) : 129 -136 . DOI: 10.13995/j.cnki.11-1802/ts.042895

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