Food and Fermentation Industries >

2026 , Vol. 52 >Issue 1: 113 - 121

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042263

Screening for uric acid-degrading microorganisms and initial exploration of their uric acid metabolic mechanisms

  • YANG Rui ,
  • YU Yang ,
  • SONG Chengying ,
  • Mandlaa ,
  • SUN Ziyu ,
  • CHEN Zhongjun
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  • (College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010000, China)

Received date: 2025-01-25

  Revised date: 2025-05-01

  Online published: 2026-01-22

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Abstract

Hyperuricemia (HUA), as a syndrome of purine metabolism disorder, has been increasing in number year by year.At present, allopurinol, a drug used to treat HUA, is nephrotoxic and can even cause death.It is necessary to find a safe method to reduce uric acid.In this study, the strain Weizmannia coagulans B8 with uric acid degradation rate of 29.12% was identified through plate screening combined with HPLC.The strain exhibits good acid resistance (pH 2.0) and bile salt tolerance (0.3% bile salt) in vitro, meanwhile has a broad-spectrum antagonistic effect on pathogenic bacteria.These characteristics provide a basis for its potential as a probiotic.The mechanism of uric acid metabolism in strain W.coagulans B8 was explored at the genetic level.Whole genome sequencing analysis revealed that the genome of W.coagulans B8 is 3 669 133 bp long, with 62 genes related to uric acid metabolism (allB, rpoC, purK, etc.) annotated and integrated into the KEGG purine metabolism pathway.The uricase activity was also measured to be (1.34±0.16) U/mL, thus elucidating the complete metabolic pathway of uric acid.The results have revealed the probiotic characteristics and uric acid metabolism related genes and pathways of W.coagulans B8, providing a theoretical basis for further application of the strain in controlling uric acid levels in food.

Key words： degradation of uric acid; Weizmannia coagulans; HPLC; whole genome; metabolic mechanism

Cite this article

YANG Rui , YU Yang , SONG Chengying , Mandlaa , SUN Ziyu , CHEN Zhongjun . Screening for uric acid-degrading microorganisms and initial exploration of their uric acid metabolic mechanisms[J]. Food and Fermentation Industries, 2026 , 52(1) : 113 -121 . DOI: 10.13995/j.cnki.11-1802/ts.042263

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