To deeply understand the microbial diversity of high-temperature Daqu (HTD) from the sauce-amora Baijiu production region in the northwestern Hubei Province, this study used MiSeq high-throughput sequencing technology to analyze it.The results showed that the dominant bacterial taxa in the HTD from this production area were mainly Kroppenstedtia (22.04%), Lentibacillus (17.82%), and Bacillus (15.81%), and the dominant fungal taxa were mainly Thermomyces (39.91%), Thermoascus (28.23%), and Aspergillus (10.10%).Operational taxonomic unit (OTU)-level analysis identified seven core bacterial OTUs, which were assigned to Kroppenstedtia (18.11%), Lentibacillus (11.72%), and Saccharopolyspora (2.93%), with a cumulative relative abundance of 32.77%.Two core fungal OTUs were assigned to Thermomyces (26.00%) and Aspergillus (5.17%), with a cumulative average relative abundance of 31.17%.Principal coordinates analysis indicated that the microbial community structures of samples from different counties still exhibited differences.In the sample from Nanzhang County, the predominant unique bacterial taxon was Bacillus (23.75%), while in the Shennongjia Forest District, the unique bacterial and fungal taxa were Thermoactinomyces (4.92%) and Rhizopus (20.70%), respectively.In Baokang County, the unique bacterial taxon was Staphylococcus (17.63%).Notably, the unique bacterial and fungal taxa and their cumulative relative abundance in the Shennongjia Forest District were higher than those in the other two counties, indicating a significant difference between the Shennongjia samples and those from the other two counties.
[1] ZHOU W, CHEN Z Y, ZHANG G H, et al.A system-level investigation into the pharmacological mechanisms of flavor compounds in liquor[J].Journal of Food Biochemistry, 2020, 44(10):e13417.
[2] 唐维川, 孔祥凯, 王婷, 等.基于主成分分析法评价酱香型白酒的不同轮次基酒[J].现代食品科技, 2021, 37(7):269-277;268.
TANG W C, KONG X K, WANG T, et al.Evaluation of different rounds of Moutai-flavor Baijiu based on principal component analysis[J].Modern Food Science and Technology, 2021, 37(7):269-277;268.
[3] GE J F, QI Y L, YAO W R, et al.Identification of trace components in sauce-flavor Baijiu by high-resolution mass spectrometry[J].Molecules, 2023, 28(3):1273.
[4] 姜政飞. 赤水河产区酱香型白酒酿造微生物来源及环境微生物分布[D].无锡:江南大学, 2023.
JIANG Z F.Microbial sources and environmental microbial distribution of the sauce-flavor Baijiu in the Chishui River producing region[D].Wuxi:Jiangnan University, 2023.
[5] 侯强川, 王玉荣, 王文平, 等.茅台和尧治河高温大曲细菌群落结构差异及功能预测[J].食品与发酵工业, 2022, 48(1):36-44.
HOU Q C, WANG Y R, WANG W P, et al.Difference of bacterial community structure and functional prediction in high-temperature Daqu of Maotai and Yaozhihe[J].Food and Fermentation Industries, 2022, 48(1):36-44.
[6] HOU Q C, WANG Y R, QU D W, et al.Microbial communities, functional, and flavor differences among three different-colored high-temperature Daqu:A comprehensive metagenomic, physicochemical, and electronic sensory analysis[J].Food Research International, 2024, 184:114257.
[7] GUO Z, XIANG F S, HOU Q C, et al.Analysis of bacterial community structure and taste quality of different colored high-temperature Daqu in the Xiangyang region, China[J].LWT, 2023, 188:115411.
[8] CAI W C, WANG Y R, NI H, et al.Diversity of microbiota, microbial functions, and flavor in different types of low-temperature Daqu[J].Food Research International, 2021, 150:110734.
[9] LI W W, FAN G S, FU Z L, et al.Effects of fortification of Daqu with various yeasts on microbial community structure and flavor metabolism[J].Food Research International, 2020, 129:108837.
[10] CHEN Y R, LI K M, LIU T, et al.Analysis of difference in microbial community and physicochemical indices between surface and central parts of Chinese special-flavor Baijiu Daqu[J].Frontiers in Microbiology, 2020, 11:592421.
[11] GUO Z, WANG Y R, XIANG F S, et al.Bacterial diversity in pickled cowpea (Vigna unguiculata[Linn.] walp) as determined by illumina MiSeq sequencing and culture-dependent methods[J].Current Microbiology, 2021, 78(4):1286-1297.
[12] 向凡舒, 蔡文超, 田龙新, 等.襄阳地区高温大曲真菌群落结构及其风味品质解析[J].食品科学, 2023, 44(24):211-219.
XIANG F S, CAI W C, TIAN L X, et al.Analysis of fungal community structure and flavor quality of high-temperature Daqu from Xiangyang, China[J].Food Science, 2023, 44(24):211-219.
[13] WANG Y R, CAI W C, WANG W P, et al.Analysis of microbial diversity and functional differences in different types of high-temperature Daqu[J].Food Science & Nutrition, 2021, 9(2):1003-1016.
[14] CAI W C, XUE Y A, WANG Y R, et al.The fungal communities and flavor profiles in different types of high-temperature Daqu as revealed by high-throughput sequencing and electronic senses[J].Frontiers in Microbiology, 2021, 12:784651.
[15] MAGOČ T, SALZBERG S L.FLASH:Fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics, 2011, 27(21):2957-2963.
[16] CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods, 2010, 7(5):335-336.
[17] WEI Z G, ZHANG X D, CAO M, et al.Comparison of methods for picking the operational taxonomic units from amplicon sequences[J].Frontiers in Microbiology, 2021, 12:644012.
[18] POLLOCK J, GLENDINNING L, WISEDCHANWET T, et al.The madness of microbiome:Attempting to find consensus “best practice” for 16S microbiome studies[J].Applied and Environmental Microbiology, 2018, 84(7):e02 627-17.
[19] BRANDT M I, TROUCHE B, QUINTRIC L, et al.Bioinformatic pipelines combining denoising and clustering tools allow for more comprehensive prokaryotic and eukaryotic metabarcoding[J].Molecular Ecology Resources, 2021, 21(6):1904-1921.
[20] PANG Z M, LI W W, HAO J, et al.Correlational analysis of the physicochemical indexes, volatile flavor components, and microbial communities of high-temperature Daqu in the northern region of China[J].Foods, 2023, 12(2):326.
[21] ZHAN E X, XU S X, CHEN X X, et al.Effects of high temperature pretreatment and inoculation of Bacillus coagulans on promoting aerobic composting of chicken manure[J].BioResources, 2023, 18(2):3089-3100.
[22] ZHU M, ZHENG J, XIE J, et al.Effects of environmental factors on the microbial community changes during medium-high temperature Daqu manufacturing[J].Food Research International, 2022, 153:110955.
[23] ZHANG Y D, DING F, SHEN Y, et al.Characteristics of the microbiota and metabolic profile of high-temperature Daqu with different grades[J].World Journal of Microbiology and Biotechnology, 2022, 38(8):137.
[24] SHI W, CHAI L J, ZHAO H, et al.Deciphering the effects of different types of high-temperature Daqu on the fermentation process and flavor profiles of sauce-flavor Baijiu[J].Food Bioscience, 2024, 61:104917.
[25] YANG L, XIAN C, LI P, et al.The spatio-temporal diversity and succession of microbial community and its environment driving factors during stacking fermentation of Maotai-flavor Baijiu[J].Food Research International, 2023, 169:112892.
[26] DENG L, MAO X, LIU D, et al.Comparative analysis of physicochemical properties and microbial composition in high-temperature Daqu with different colors[J].Frontiers in Microbiology, 2020, 11:588117.
[27] ZHANG H X, WANG L, WANG H Y, et al.Effects of initial temperature on microbial community succession rate and volatile flavors during Baijiu fermentation process[J].Food Research International, 2021, 141:109887.
