Food and Fermentation Industries >

2026 , Vol. 52 >Issue 3: 148 - 157

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043119

Enzymatic properties of xylanase immobilized by yeast spores

  • ZOU Yan ,
  • YANG Shuheng ,
  • ZHANG Jingtao ,
  • ZENG Xueying ,
  • PAN Cong ,
  • ZHOU Fang ,
  • YANG Zongling ,
  • DUAN Shenglin ,
  • LIU Guoyu ,
  • YUAN Peng
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  • (China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, Beijing 100015, China)

Received date: 2025-04-25

  Revised date: 2025-06-04

  Online published: 2026-03-13

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Abstract

Xylanase, a key enzyme involved in hemicellulose degradation, plays a pivotal role in biomass conversion, food processing, and papermaking industries.However, the industrial application of free xylanase is often restricted due to its poor thermal stability, narrow pH adaptability, and limited reusability.To address these limitations, a novel immobilization strategy was developed by anchoring Aspergillus niger-derived xylanase XynB onto the spore surface of Saccharomyces cerevisiae, leveraging the unique structural characteristics of yeast spores.The immobilized enzyme exhibited enhanced stability through spatial hindrance and electrostatic interactions with the spore carrier.The surface expression and localization of XynB on spores were verified by SDS-PAGE and fluorescence microscopy.Enzymatic properties were systematically investigated using wild-type AN120 and spore wall-deficient mutants osw2Δ and dit1Δ.Among them, the osw2Δ-spore-displayed XynB exhibited superior catalytic performance, with optimal activity at 40 ℃ and pH 5.0.Compared with the free enzyme, the immobilized form retained about 30% of its initial activity after incubation at 80 ℃ for 120 min, and >50% activity was maintained after 120 min within the pH range of 4.0-8.0, indicating significantly improved thermostability and pH tolerance.Moreover, the immobilized enzyme showed excellent resistance to metal ions, EDTA, detergents (Triton X-100, SDS), and high salinity.After five reuse cycles, 56% of the initial activity was retained.Notably, the immobilized XynB demonstrated high substrate specificity, particularly toward birch xylan.These findings provide a promising strategy for xylanase immobilization and offer valuable insights for its application in xylan hydrolysis and lignocellulosic biomass conversion.

Key words： Saccharomyces cerevisiae; spore immobilization; xylanase; enzymatic property

Cite this article

ZOU Yan , YANG Shuheng , ZHANG Jingtao , ZENG Xueying , PAN Cong , ZHOU Fang , YANG Zongling , DUAN Shenglin , LIU Guoyu , YUAN Peng . Enzymatic properties of xylanase immobilized by yeast spores[J]. Food and Fermentation Industries, 2026 , 52(3) : 148 -157 . DOI: 10.13995/j.cnki.11-1802/ts.043119

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