Food and Fermentation Industries >

2026 , Vol. 52 >Issue 4: 28 - 39

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042778

Effect of different extraction methods on physicochemical properties and biological activity of polysaccharides obtained from Berberis dasystachya Maxim

  • XIN Xiaoming ,
  • YUE Qingming ,
  • HAN Lijuan ,
  • LUAN Wenli ,
  • DENG Yongrong ,
  • MA Nana
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  • (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

Received date: 2025-03-24

  Revised date: 2025-07-03

  Online published: 2026-03-17

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Abstract

Taking Qinghai’s characteristic resources of Berberis dasystachya Maxim as raw material, hot water extraction, ultrasonic auxiliary hot water extraction, microwave auxiliary hot water extraction, ultrasonic synergy enzymatic, microwave synergy enzymatic, and hot water coenzyme method six methods were used to prepare Berberis dasystachya polysaccharides (BDPs).The structural characteristics of the extracted BDPs were analyzed by Fourier transform infrared spectroscopy (FT-IR), ultraviolet spectroscopy (UV), scanning electron microscope (SEM), and Congo red test.The bioactivities were evaluated through antioxidant capacity assays and in vitro hypoglycemic activity tests.Results showed that the six BDPs prepared by different extraction methods all contained the key functional groups of polysaccharides, and the polysaccharides were in the pyranose configuration linked by α and β glycosidic bonds.SEM showed that the apparent structures of the polysaccharides obtained by different extraction methods were different.Compared with other extraction methods, BDPs extracted by ultrasonic synergy enzymatic (BDPs-UEE) had the highest yield, and the polysaccharide content was as high as 75.96% (P<0.05).The determination results of the scavenging abilities of DPPH free radicals and ABTS cationic radicals, as well as the inhibitory rates of the activities of α-glucosidase and α-amylase, indicated that all six types of BDPs exhibited antioxidant and hypoglycemic activities to varying degrees.Among them, BDPs-UEE showed relatively better antioxidant and hypoglycemic activities.Therefore, the ultrasonic synergy enzymatic method could be regarded as the optimal method for extracting BDPs.This study could lay a certain foundation for the development and utilization of BDPs.

Key words： Berberis dasystachya polysaccharides; extraction method; physicochemical properties; antioxidant activity; hypoglycemic activity

Cite this article

XIN Xiaoming , YUE Qingming , HAN Lijuan , LUAN Wenli , DENG Yongrong , MA Nana . Effect of different extraction methods on physicochemical properties and biological activity of polysaccharides obtained from Berberis dasystachya Maxim[J]. Food and Fermentation Industries, 2026 , 52(4) : 28 -39 . DOI: 10.13995/j.cnki.11-1802/ts.042778

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