A long-term high-fat diet causes fat accumulation, abnormal blood lipids, and gut microbiota dysbiosis.It also increases the incidence of chronic diseases such as hyperlipidemia.Aimed to investigate the effects of inulin combined with Auricularia auricula polysaccharides(AAP) on serum indices, hepatic lipid deposition, and gut microbiota in high-fat diet-fed mice.The study first evaluated the hypolipidemic activity of inulin and AAP mixtures at different ratios (5∶0, 4∶1, 3∶2, 2∶3, 1∶4, 0∶5) using in vitro assays.Mice were divided into a normal control group fed a standard diet, a high-fat model group fed a high-fat diet, and intervention groups fed the high-fat diet supplemented with the different polysaccharide mixtures.After 8 weeks of intervention, the study examined the effects of the high-fat diet on serum parameters, liver tissue, and gut microbiota in the mice.Results showed that the inulin-AAP mixture at a 2∶3 ratio achieved the highest binding rates for both sodium glycocholate and sodium taurocholate.All polysaccharide mixtures significantly reduced body weight and liver index in the experimental mice.Serum levels of TC, TG, and LDL-C decreased markedly, while HDL-C levels increased significantly.Furthermore, the number and size of lipid droplets in the liver tissue were substantially reduced.The combined intervention demonstrated more pronounced effects than using either inulin or AAP alone.Intervention with the inulin-AAP mixture increased the relative abundance of short-chain fatty acid-producing bacteria.These included genera such as Lactobacillus, Odoribacter, Parabacteroides, and Bacteroides.The mixture also decreased the relative abundance of Desulfovibrio, Helicobacter, Ruminococcus torques, and Colidextribacter.This study indicated that inulin combined with AAP regulates blood lipids and improves gut microbiota in high-fat diet-fed mice.The findings offer a new perspective for developing strategies to prevent hyperlipidemia and create related functional products.
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